Molecular detection of disease-causing agent infecting Malvastrum coromandelianum L. in Bahawalpur District Punjab Pakistan


  • Ali HASNAIN Government College University, Department of Botany, Lahore 54000 (PK)
  • Hsi-Hsien YANG Chaoyang University of Technology, Department of Environmental Engineering and Management (TW)
  • Muhammad Zia-UR-REHMAN Government Graduate College of Science, Wahdat road Lahore (PK)
  • Sohaib MOHAMMAD Government College University, Department of Botany, Lahore 54000 (PK)
  • Zahid MEHMOOD Government College University, Department of Botany, Lahore 54000 (PK)
  • Ayesha IQBAL Government College University, Department of Botany, Lahore 54000 (PK)
  • Arooba JOHN Government College University, Department of Botany, Lahore 54000 (PK)
  • Binyameen Government College University, Department of Botany, Lahore 54000 (PK)
  • Maryam IQBAL Government College University, Department of Botany, Lahore 54000 (PK)
  • Naveed ANJUM Government College University, Department of Botany, Lahore 54000 (PK)
  • Iftikhar HUSSAIN University of Vigo, Department of Plant Biology and Soil Science, 36310-Vigo (ES)
  • Zafar I. KHAN University of Sargodha, Department of Botany, Sargodha (PK)
  • Muhammad U.F. AWAN Government College University, Department of Botany, Lahore 54000 (PK)



Alpha and beta satellite, Begomoviruse, Bemisia tabaci, Geminiviridae, Malvastrum coromandelianum


Weeds are common ancestors of modern crops. They are critical source of genes for resistance to diseases, pests and stresses such as drought and extreme environmental conditions. The wild relatives led to improve resistance to wheat curl mite, late blight in potato and to grassy stunt disease in rice. Agricultural pathogens are infecting economically important cash crops and weeds as well. Malvastrum coromandelianum is highly invasive weed specie of family Malvaceae. It is distributed all over the world in all climatic condition of tropical, sub-tropical and temperate region. The Malvaceae family is economically important as cotton (Gossipium hirsutem) belongs to it. M. coromandelianum has ethnobotanical importance and is extensively applied in the treatment of different diseases in south Punjab of Pakistan. It has been observed that there is a specific disease that affects the leaves of M. coromandelianum. In this study, we investigated the disease-causing agents (viruses and bacteria) at molecular level. Molecular investigation includes DNA isolation of unknown pathogens, verification through agarose gel electrophoresis, for identification of bacterial pathogens, 16s RNA primers were applied, and for viral pathogen using specific primers and RCA (rolling circle amplification) product as template. PCR was carried out for the amplification of full-length genomes. Manifestation of 1.4 kb bands showed the presence of the alpha and beta satellite of begomoviruse. Two types of begomoviruses, Malvestrum yellow vain younnan virus (MaYVYV) and Malvastrum yellow vain virus (MaYVV) was confirmed in this weed. The intermediate host Bemisia tabaci is the most common for the begomovirus transmission in crops and weeds. Intercrops and trap-crops techniques decrease the rate of whitefly population. Cultural methods, use of chemicals and biocontrol are useful practices to eliminate whitefly from field.


Adams MJ, Kefkowitz EJ, King AMQ, Harrach B, Harrison RL, Knowles NJ (2017). Changes to taxonomy and the international code of virus classification and nomenclature ratified by the international committee on taxonomy of viruses. Archives of Virology 162:2505-2538.

Briddon RW, Martin DP, Roumagnac P, Navas-Castillo J, Fiallo-Olivé E, Moriones E (2018). Alphasatellitidae: a new family with two subfamilies for the classification of geminivirus- and nanovirus-associated alphasatellites. Archives of Virology 163:2587-600.

Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF (2015). Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Archives of Virology 160:1593-619.

Dosso K, N’guessan BB, Bidie AP, Gnangoran BN, Méité S, N’guessan D, Yapo AP, Ehilé EE (2012). Antidiarrhoeal activity of an ethanol extract of the stem bark of Piliostigma reticulatum (Caesalpiniaceae) in rats. African Journal of Traditional, Complementary and Alternative Medicines 9(2):242-249.

Fiallo-Olivé E, Lett JM, Martin DP, Roumagnac P, Varsani A, Zerbini FM, Navas-Castillo J, ICTV Report Consortium (2021). ICTV Virus Taxonomy Profile: Geminiviridae 102:001696.

Fiallo-Olivé E, Martínez-Zubiaur Y, Moriones E, Navas-Castillo J (2012). A novel class of DNA satellites associated with New World begomoviruses. Virology 426:1-6.

Fondong VN (2013). Geminivirus protein structure and function. Molecular Plant Pathology 14:635-649.

Gangrade NK, Sheorey RV, Rawal H, Chouhan S (2012). Investigation of wound healing activity of Malvastrum tricuspidatum syn and Malvastrum coromandelianum on experimental animals. International Journal of Pharmacy and Life Sciences 3(12):2259-2264.

Goodman RM (1977). Single stranded-DNA genome in a whitefly-transmitted plant virus. Cell. Molecular Life Science 56:313-329. (77)90220-3

Hea YZ, Wanga YM, Yina TY, Fiallo-Olivéb E., Liua YQ, Hanley-Bowdoinc L, Wanga XW (2020). A plant DNA virus replicates in the salivary glands of its insect vector via recruitment of host DNA synthesis machinery. Proceeding of National Academy of Sciences USA 117:16928-16937.

Khonsung P, Nantsupawat S, Jesadanont SN, Chantharateptawan V, Panthong A (2006). Anti-inflammatory and analgesic activities of water extract of Malvastrum coromandelianum (L.) Garcke. Thai Journal of Pharmacology 28(3):8-15.

Kumar SS, Thangaraj M, Kathiresan K (2012). DNA extraction protocol for plants with high levels of secondary metabolites and polysaccharides without using liquid nitrogen and phenol. International Scholarly Research Network 1: 6.

Lee PY, Costumbrado J, Hsu CY, Kim YH (2012). Agarose gel electrophoresis for the separation of DNA fragments. Journal of visualized experiments: Journal of Visualized Experiments JoVE (62):3923.

Li TS (2006). Taiwanese native medicinal plants: phytopharmacology and therapeutic values. CRC Press. Taylor & Francis, London.

Lozano G, Trenado HP, Fiallo-Olivé E, Chirinos D, Geraud-Pouey F, Briddon RW (2016). Characterization of non-coding DNA satellites associated with sweepoviruses (genus Begomovirus, Geminiviridae) - definition of a distinct class of begomovirus-associated satellites. Frontiers in Microbiology 7:162.

Morris R (2015). Spectrophotometry. Current Protocols Essential Laboratory Techniques 11(1):2-1.

Navas-Castillo J, Fiallo-Olivé E (2021). Geminiviruses (Geminiviridae). In: Zuckerman M, Bamford D (Eds). Encyclopedia of Virology. Oxford: Academic Press, pp 3:411-419.

Palumboa JC, Horowitzb AR, Prabhakerc N (2001). Insecticidal control and resistance management for Bemisia tabaci. Crop Protection 20(9):739-765.

Pandey DR (2016). Optimization of morphological studies on Malvestrum corromandelianum L. Garcke in different selected sites of Rewa, Madhya Pradesh. World Journal of Pharmaceutical Research 5:1198-1207.

Rahman MU, Khan AQ, Rahmat Z, Iqbal MA, Zafar Y (2017). Genetics and genomics of cotton leaf curl disease, its viral causal agents and whitefly vector: a way forward to sustain cotton fiber security. Frontiers in Plant Science 8:1157.

Rampersad SN, Umaharan P (2007). Infecting dicotyledonous weeds in Trinidad, APS Publication. Plant Diseases 87:5.

Rehman S (2021). Cotton and Products Annual. United States Department of Agriculture. Report Number: PK2021-0004.

Roberts IM (1989). Indian cassava mosaic virus, Ultra structure of infected cells. Journal of General Virology 70:2729-2739.

Sambrook J, Russell DWR (2001). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York 1(1):162.

Shaiphali S, Rawat DS, Rao PB (2020). Malvastrum coromandelianum (L.) Garcke: An invasive weed with multiple ethnopharmacological properties. International Journal of Pharmacognosy and Phytochemical Research 12(1):16-22.

Sittiwet C, Jesadanont S, Pongpech P, Naenna P, Pongsamart S (2008). Antibacterial activity of Malvastrum coromandelianum Garcke against methicillin-sensitive and methicillin-resistant strains of Staphylococcus aureus. Current Research Bacteriology 1(1):42-45.

Wang Y, Zhang X, Hu T, Zhou X (2021). Identification of two distinct begomoviruses infecting Malvastrum corromandelianum. Phytopathology Research 3:8.

Woese C, Fox GE (1977). Phylogenetic structure of the prokaryotic domain: the primary kingdoms. Proceedings of the National Academy of Sciences of the United States of America 74(11):5088-5090.

Yadav R, Tikar SN, Sharma AK, Tyagi V, Sukumaran D, Jain AK, Veer V (2015). Screening of some weeds for larvicidal activity against Aedes albopictus, a vector of dengue and chikungunya. Journal of Vector Borne Diseases 52(1):88-94.

Zerbini, FM, Briddon RW, Idris A, Martin DP, Moriones E, Navas-Castillo J (2017). ICTV virus taxonomy profile: geminiviridae. Journal of General Virology 98:131-133.

Zhang SC, Ling KS (2011). Genetic diversity of sweet potato begomoviruses in the United States and identification of a natural recombinant between sweet potato leaf curl virus and sweet potato leaf curl Georgia virus. Archives of Virology 156(6):955-968.

Zia-Ur-Rehman M, Herrmann HW, Hameed U, Haider MS, Brown JK (2013). Advances in understanding begomovirus satellites. Annual Review of Phytopathology 51:357-581.

Zia-Ur-Rehman M, Iqbal MJ, Hameed U, Haider MS (2018). First detection of Hollyhock leaf curl virus in Malvastrum coromandelianum in Pakistan. Plant Disease 1:256-256.



How to Cite

HASNAIN, A., YANG, H.-H., Zia-UR-REHMAN, M., MOHAMMAD, S., MEHMOOD, Z., IQBAL, A., JOHN, A., Binyameen, IQBAL, M., ANJUM, N., HUSSAIN, I., KHAN, Z. I., & AWAN, M. U. (2022). Molecular detection of disease-causing agent infecting Malvastrum coromandelianum L. in Bahawalpur District Punjab Pakistan. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(3), 12797.



Research Articles
DOI: 10.15835/nbha50312797

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