Assessment of the virus infections occurrence in new established plum and sweet cherry orchards in Transylvania, Romania

Authors

  • Luminița A. ZAGRAI Fruit Research and Development Station Bistrița, 3 Drumul Dumitrei Nou, Bistrița, 420127 (RO)
  • Ioan ZAGRAI Fruit Research and Development Station Bistrița, 3 Drumul Dumitrei Nou, Bistrița, 420127 (RO)
  • Georgeta M. GUZU Fruit Research and Development Station Bistrița, 3 Drumul Dumitrei Nou, Bistrița, 420127 (RO)
  • Smaranda D. ROȘU-MAREȘ Fruit Research and Development Station Bistrița, 3 Drumul Dumitrei Nou, Bistrița, 420127 (RO)
  • Claudiu MOLDOVAN Fruit Research and Development Station Bistrița, 3 Drumul Dumitrei Nou, Bistrița, 420127 (RO)

DOI:

https://doi.org/10.15835/nbha50212734

Keywords:

stone fruits, survey, virus incidence, young orchards

Abstract

Sixteen plum and eleven sweet cherry newly established commercial orchards in Transylvania, Romania, were the subject of the survey during the vegetative periods of 2020-2021 in order to assess the occurrence of different virus infections. Two blocks of 200 trees from each orchard were monitored by visual observation for virus-like symptoms, especially for Plum pox virus (PPV) in plum orchards. Twenty trees of each plum orchard were then sampled and tested for serological detection of PPV, Prune dwarf virus (PDV), Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV) and Myrobalan latent ringspot virus (MLRSV). Similarly, ten trees of each sweet cherry orchard were sampled and tested for the presence of PDV, PNRSV, ApMV, ACLSV, PPV, Arabis mosaic virus (ArMV), Cherry leaf roll virus (CLRV), Raspberry ringspot virus (RpRSV), Strawberry latent ringspot virus (SLRSV) and Tomato black ring virus (TBRV) by serologic assays. Additionally, a few sweet cherry trees suspected to be infected by Little cherry virus-1 (LChV-1) were tested by molecular assay. Unexpectedly, no plum orchards were found to be free of PPV.  The average level of PPV infection was 32%. PNRSV occurred in 8.1% and PDV in 1.2% of sampled plum trees. Four out of ten viruses were detected in sweet cherry sampled: PDV (3.6%), ACLSV (0.9%), RpRSV (0.9%) and TBRV (0.9%). No infection with ACLSV, ApMV and MLRSV in plum and PPV, PNRSV, ApMV, ArMV, CLRV, SLRSV and LChV-1 in sweet cherry was detected. Mixed infections occurred at 4.8% in plum, and at 0.9% in sweet cherry trees sampled. The average occurrence of viruses in plum and sweet cherry orchards surveyed in Transylvania was determined at 41.3%, and 6.3% respectively. Overall results revealed a critical situation especially in regards to PPV infections making the success of fruit production in the most surveyed plum orchards quite problematic. Virus infections level in the younger sweet cherry orchards were significantly lower.

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References

Amari K, Burgos L, Pallas V, Sanchez-Pina MA (2009). Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling. Journal of General Virology 90(7):1767-1774. https://doi.org/10.1099/vir.0.009647-0 DOI: https://doi.org/10.1099/vir.0.009647-0

Atanasoff D (1932). Plum pox. A new virus disease. Yearbook University of Sofia, Faculty of Agriculture and Silviculture 11:49-69.

Bajet NB, Unruh TR, Druffel KL, Eastwell KC (2008). Occurrence of two little cherry viruses in sweet cherry in Washington State. Plant Diseases 92(2):234-238. https://doi.org/10.1094/PDIS-92-2-0234 DOI: https://doi.org/10.1094/PDIS-92-2-0234

Barba M, Hadidi A, Candresse T, Cambra M (2011). Plum pox virus. In: Hadidi A, Barba M, Candresse T, Jelkmann W (Eds). Virus and Virus-like Disease of Pome and Stone Fruits. Paul (MN): APS Press, Minnesota pp 185-197. DOI: https://doi.org/10.1094/9780890545010.036

Barba M, Pasquini G, Quacquarelli A (1986). Role of seeds in the epidemiology of two almond viruses. Acta Horticulturae 193:127-130. https://doi.org/10.17660/ActaHortic.1986.193.21 DOI: https://doi.org/10.17660/ActaHortic.1986.193.21

Borisova A, Kamenova I, Krumov S (2021). Incidence of ilarviruses in cherry nurseries and newly established orchards. Journal of Central European Agriculture 22(4):771-776. https://doi.org/10.5513/JCEA01/22.4.3286 DOI: https://doi.org/10.5513/JCEA01/22.4.3286

Caglayan K, Ulubas-Serce C, Gazel M, Varveri C (2011). Prune dwarf virus. In: Hadidi A, Barba M, Candresse T, Jelkmann W (Eds). Virus and virus-like diseases of pome and stone fruits. Paul (MN): APS Press, Minnesota pp 199-205. DOI: https://doi.org/10.1094/9780890545010.037

Candresse T, Cambra M, Dallot S, Lanneau M, Asensio M, Gorris MT, Revers F, Macquaire G, Olmos A, Boscia D, Quiot JB (1998). Comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the D and M serotypes of plum pox potyvirus. Phytopathology 88(3):198-204. https://doi.org/10.1094/PHYTO.1998.88.3.198 DOI: https://doi.org/10.1094/PHYTO.1998.88.3.198

Cambra M, Capote N, Myrta A, Llácer G (2006). Plum pox virus and the estimated costs associated with sharka disease. Bulletin EPPO 36:202-204. https://doi.org/10.1111/j.1365-2338.2006.01027.x DOI: https://doi.org/10.1111/j.1365-2338.2006.01027.x

Cameron HR, Milbrath JA, Tate LA (1973). Pollen transmission of Prunus necrotic ringspot virus in prune and sour cherry orchards. Plant Disease Reporter 57:241-243.

Clark M, Adams AN (1977). Characteristic of the microplate method of enzyme linked immunosorbent assay (ELISA) for detection of plant viruses. Journal of General Virology 34:475-483. DOI: https://doi.org/10.1099/0022-1317-34-3-475

Dallot S, Gottwald T, Labonne G, Quiot JB (2004). Factors affecting the spread of Plum pox virus strain M in peach orchards subjected to roguing in France. Phytopathology 94(12):1390-1398. https://doi.org/10.1094/PHYTO.2004.94.12.1390 DOI: https://doi.org/10.1094/PHYTO.2004.94.12.1390

Delbos RP, Dunez J (1988). Apple chlorotic leaf spot virus. In: European Handbook of Plant Disease. Blackwell Scientific Publication, Oxford.

Desvignes JC, Boye R (1988). Different diseases caused by the chlorotic leaf spot virus on the fruit trees. Acta Horticulturae 235:31-38. https://doi.org/10.17660/ActaHortic.1989.235.3 DOI: https://doi.org/10.17660/ActaHortic.1989.235.3

Digiaro M., Savino V, Di Terlizzi, B. (1992). Ilaviruses in Apricot and Plum Pollen. Acta Horticulturae 309:93-98. https://doi.org/10.17660/ActaHortic.1992.309.10 DOI: https://doi.org/10.17660/ActaHortic.1992.309.10

Digiaro M, Bici I, Myrta A, Di Terlizzi B, Savino V (1994). A survey on virus and virus diseases of sweet and sour cherry in Albania. Phytopathologia Mediterranea 33:162-164. https://www.jstor.org/stable/42685939

Dunez J, Sutic D (1988). Plum pox virus. In: Smith IM, Dunez J, Eliot RA, Phillips DH, Arches SA (Eds). European Handbook of Plant Diseases. pp 44-46.

Eastwell KC (1997). Little cherry disease - in perspective. In: Recent Research Development in Plant Pathology – Filamentous Viruses of Woody Plants. D. Vesely, ed. Research Signpost, India, pp 143-151.

EPPO (2001a). Certification scheme for cherry. EPPO Bulletin 31:447-461. DOI: https://doi.org/10.1111/j.1365-2338.2001.tb01027.x

EPPO (2001b). Certification scheme for almond, apricot, peach and plum. EPPO Bulletin 31:463-478. DOI: https://doi.org/10.1111/j.1365-2338.2001.tb01028.x

EPPO (2021). EPPO Global database. In: EPPO Global database, Paris, France: EPPO. https://gd.eppo.int/

Food and Agriculture Data (FAOSTAT-2020). Retrieved 2022 March 10 from https://www.fao.org/faostat/en/#data/QCL

Glasa M, Veronique MJ, Labone G, Subr Z, Kudela O, Quiot JB (2002). A natural population of recombinant Plum pox virus is viable and competitive under field conditions. European Journal of Plant Pathology 108(9):843-853. https://doi.org/10.1023/A:1021294221878 DOI: https://doi.org/10.1023/A:1021294221878

Gospodaryk A, Moroèko-Bièevska I, Pûpola N, Kâle A (2013). Occurrence of stone fruit viruses in plum orchards in Latvia. Proceedings of the Latvian Academy of Sciences. Section B 67(2):116-123. https://doi.org/10.2478/prolas-2013-0018 DOI: https://doi.org/10.2478/prolas-2013-0018

Hadidi A, Barba M (2011). Economic impact of pome and stone fruit viruses and viroids. In: Hadidi A, Barba M, Candresse T, Jelkmann W (Eds). Virus and Virus-like Disease of Pome and Stone Fruits. Paul (MN): APS Press, Minnesota pp 1-7. DOI: https://doi.org/10.1094/9780890545010.001

Hammond RW (2011). Prunus necrotic ringspot virus. In: Hadidi A, Barba M, Candresse T, Jelkmann W (Eds). Virus and Virus-like Disease of Pome and Stone Fruits. Paul (MN): APS Press, Minnesota pp 207-213. DOI: https://doi.org/10.1094/9780890545010.038

Isogai M, Aoyagi J, Nakagawa M, Kubodera Y, Satoh K, Katoh T, Inamori M, Yamashita K, Yoshikawa N (2004). Molecular detection of five cherry viruses from sweet cherry trees in Japan. Journal of General Plant Pathology 70:288-291. https://doi.org/10.1007/s10327-004-0129-4 DOI: https://doi.org/10.1007/s10327-004-0129-4

Kamenova I, Borisova A, Popov A (2019). Incidence and genetic diversity of Prune dwarf virus in sweet and sour cherry in Bulgaria. Biotechnology & Biotechnological Equipment 33(1):980-987. https://doi.org/10.1080/13102818.2019.1637278 DOI: https://doi.org/10.1080/13102818.2019.1637278

Kerlan C, Dunez J (1979). Biological and serological differentiation of strains of Sharka virus. Annales de Phytopathologie 11:241-250.

Labonne G, Yvon M, Quiot J, Avinent L, Llácer G (1995). Aphids as potential vectors of Plum pox virus: comparison of methods of testing and epidemiological consequences. Acta Horticulturae 386:207-218. https://doi.org/10.17660/ActaHortic.1995.386.27 DOI: https://doi.org/10.17660/ActaHortic.1995.386.27

Mandic B, Matic S, Al Rwahnih M, Jelkmann W, Myrta A (2007). Viruses of sweet and sour cherry in Serbia. Journal of Plant Pathology 89(1):103-108. https://www.jstor.org/stable/41998362

Martelli GP, Uyemoto JK (2011). Nematode-borne viruses of stone fruits. In: Hadidi A, Barba M, Candresse T, Jelkmann W (Eds). Virus and Virus-like Disease of Pome and Stone Fruits. Paul (MN): APS Press, Minnesota pp 161-170. DOI: https://doi.org/10.1094/9780890545010.032

Matic S, Al Rwahnih M, Myrta A, Duric G (2008). Viruses of stone fruits in Bosnia and Herzegovina. Acta Horticulturae 781:71-74. https://doi.org/10.17660/ActaHortic.2008.781.8 DOI: https://doi.org/10.17660/ActaHortic.2008.781.8

Maxim A, Papp J (2000). Modificări biochimice produse de virusurile ILAR (prune dwarf și Prunus necrotic ring spot) în fructele de cireș [Biochemical changes caused by ILAR viruses (prune dwarf and Prunus necrotic ring spot) in sweet cherry fruits] Simpozionul Stiințific Anual Cluj-Napoca, 196-200.

Maxim A, Zagrai I, Isac M (2002). Detection of Plum Pox with Sweet Cherry in Romania. Middle European Meeting on Plum Pox, Piteşti-Mărăcineni, Plant’s Health Magazine 6:48-51.

Mink GI (1992) Ilarvirus Vectors. In: Harris KF (Ed). Advances in Disease Vector Research. vol 9. Springer, New York, NY. DOI: https://doi.org/10.1007/978-1-4612-2910-0_8

Myrta A, Di Terlizzi B, Savino V, Martelli GP (2003). Virus diseases affecting the Mediterranean stone fruit industry: a decade of surveys. In: Myrta A, Di Terlizzi B, Savino V (Eds). Virus and virus-like diseases of stone fruits, with particular reference to the Mediterranean region. Bari: CIHEAM Options Méditerranéennes, Série B. Etudes et Recherches; n. 45, pp 15-23.

Myrta A, Matic S, Malinowski T, Pasquini G, Candresse T (2011). Apple chlorotic leaf spot virus in stone fruits. In: Hadidi A, Barba M, Candresse T, Jelkmann W (Eds). Virus and Virus-like Disease of Pome and Stone Fruits. Paul (MN): APS Press, Minnesota pp 85-90. DOI: https://doi.org/10.1094/9780890545010.017

Nassar E, Choueiri E, Minoia S, Di Serio F, Djelouah K (2012). First survey on pome fruit viruses in Libanon. Petria 22(3):191.

Nemeth M (1986). Virus, mycoplasma and rickettsia diseases of fruit trees. Akademiai Kiado, Budapest.

Nemeth M (1994). History and importance of plum pox in stone-fruit production. Bulletin OEPP 24(3):525-536. https://doi.org/10.1111/j.1365-2338.1994.tb01065.x DOI: https://doi.org/10.1111/j.1365-2338.1994.tb01065.x

Ni H, Yansu S, Xiaofang M, Guojun H, Liping W, Guoping W (2012). Incidence of viral diseases on pear plants and the molecular characterization of three pear viruses in China. Petria 22(3):150-158.

Nicaise V (2014). Crop immunity against viruses: outcomes and future challenges. Frontiers in Plant Science 5:660. https://doi.org/10.3389/fpls.2014.00660 DOI: https://doi.org/10.3389/fpls.2014.00660

Pallas V, Aparicio F, Herranz MC, Amari K, Sanchez-Pina MA, Myrta A, Sanchez-Navarro JA (2012). Ilarviruses of Prunus spp.: A continued concern for fruit trees. Phytopathology 102(12):1108-1120. https://doi.org/10.1094/PHYTO-02-12-0023-RVW DOI: https://doi.org/10.1094/PHYTO-02-12-0023-RVW

Paunovic S, Paquini G, Barba M (2011). Apple mosaic virus in stone fruits. In: Hadidi A, Barba M, Candresse T, Jelkmann W (Eds). Virus and Virus-like Disease of Pome and Stone Fruits. Paul (MN): APS Press, Minnesota pp 91-95. DOI: https://doi.org/10.1094/9780890545010.018

Pavliuk L, Riaba I, Udovychenko K, Tria¬pitsyna N, Bublyk M (2019). Phyto-virologic state of parent plantings of cherry and mazzard cherry in Ukraine. Bulletin of Agricultural Science 97(7):20-26. https://agrovisnyk.com/pdf/en_2019_07_03 DOI: https://doi.org/10.31073/agrovisnyk201907-03

Plopa C, Butac M (2020). Assesment of the phytoviral status of some plum orchards in the Muntenia region. Fruit Growing Research XXXVI:83-91. https://doi.org/10.33045/fgr.v36.2020.10 DOI: https://doi.org/10.33045/fgr.v36.2020.10

Plopa C, Iancu A, Butac M (2021). The incidence of TBRV and ArMV in new cherry plantation from southern area of Romania. Fruit Growing Research XXXVII:136-140. DOI: https://doi.org/10.33045/fgr.v37.2021.19

Reed PJ, Foster JA (2011). Exclusion of pome and stone fruit viruses, viroids, and phytoplasmas by certification and quarantine. In: Hadidi A, Barba M, Candresse T, Jelkman W (Eds). Virus and Virus-Like Diseases of Pome and Stone Fruits. APS Press, Minnesota pp 381-388. DOI: https://doi.org/10.1094/9780890545010.064

Rott ME, Jelkmann W (2001). Detection and partial characterization of a second closterovirus associated with Little Cherry Disease, Little cherry virus-2. APS 91(3):261-267. https://doi.org/10.1094/PHYTO.2001.91.3.261 DOI: https://doi.org/10.1094/PHYTO.2001.91.3.261

Rott ME, Jelkmann W (2005). Little cherry virus-2: Sequence and genomic organization of an unusual member of the Closteroviridae. Archives of Virology 150:107-123. https://doi.org/10.1007/s00705-004-0382-z DOI: https://doi.org/10.1007/s00705-004-0382-z

Roy AS, Smith IM (1994). Plum pox situation in Europe. Bulletin EPPO 24:515-523. DOI: https://doi.org/10.1111/j.1365-2338.1994.tb01064.x

Roy AS (2011). Control measures of pome and stone fruit viruses, viroids, and phytoplasmas: role of international organizations. In: Hadidi A, Barba M, Candresse T, Jelkman W (Eds). Virus and Virus-Like Diseases of Pome and Stone Fruits. APS Press, Minnesota, pp 407-413. DOI: https://doi.org/10.1094/9780890545010.068

Scholthof KB, Adkins S, Czosnek H, Palukaitis P, Jacquot E, Hohn T, Hohn B (2011). Top 10 plant viruses in molecular plant pathology. Molecular Plant Pathology 12:938-954. https://doi.org/10.1111/J.1364-3703.2011.00752.X DOI: https://doi.org/10.1111/j.1364-3703.2011.00752.x

Schroder M, Petruschke M (2010). Occurrence of Little cherry virus-1 on Prunus species in the State of Baden-Wurttemberg, Germany. Julius-Kuhn-Archiv 427:268-271.

Scorza R, Callahan A, Dardick C, Ravelonandro M, Polak J, Malinowski T, Zagrai I, Cambra M, Kamenova V (2013). Genetic engineering of Plum pox virus resistance – ‘HoneySweet’ plum - from concept to product. Journal of Plant Biotechnology 115(1):1-12. https://doi.org/10.1007/s11240-013-0339-6 DOI: https://doi.org/10.1007/s11240-013-0339-6

Ulubas C (2008). Viral diseases diagnosed by DAS-ELISA and RT-PCR of sour and sweet cherry trees from different provinces of Turkey. Acta Horticulturae 795:909-911. https://doi.org/10.17660/ActaHortic.2008.795.147 DOI: https://doi.org/10.17660/ActaHortic.2008.795.147

Ulubas Serce C, Ertunc F (2008). Virus infections in stone fruit trees from different provinces of Turkey. Acta Horticulturae 781:89-92. https://doi.org/10.17660/ActaHortic.2008.781.11 DOI: https://doi.org/10.17660/ActaHortic.2008.781.11

Uyemoto JK, Grant JA, Krueger WH, Olson WH, Osgood JW, Sibbet GS, Viveros M, Weakley CV (1989). Survey detects viruses in almond, prune, and sweet cherry orchards. California Agriculture 43:14-15.

Uyemoto J K, Scott SW (1992). Important diseases of Prunus caused by viruses and other graft-transmissible pathogens in California and South Carolina. Plant Diseases 76:5-11. https://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1992Articles DOI: https://doi.org/10.1094/PD-76-0005

Ventura J A, Lima I DM, Martins MVV, Culik MP, Costa H (2019). Impact and management of diseases in the propagation of fruit plants. Revista Brasileira de Fruticultura 41. DOI: https://doi.org/10.1590/0100-29452019647

Yardimci BCN, Culal-Kilic H (2011). Detection of viruses infecting stone fruits in Western Mediterranean region of Turkey. The Plant Pathology Journal 27(1):44-52. https://doi.org/10.5423/PPJ.2011.27.1.044 DOI: https://doi.org/10.5423/PPJ.2011.27.1.044

Yorston JM, McMullen RD, Slykhuis JT, Welsh MF (1981). Little cherry disease in British Columbia. British Columbia Ministry of Agriculture and Food 8:1-4.

Zagrai I, Zagrai L, Preda S, Isac M, Cardei E (2010a). Incidence of Plum pox virus in Romanian plum orchards. Bulletin UASVM Horticulture 67(1-2):488. http://dx.doi.org/10.15835/buasvmcn-hort:4939

Zagrai I, Zagrai L, Kelemen B, Petricele I, Pamfil D, Popescu O, Preda S, Briciu A (2010b). Typing and distribution of Plum pox virus isolates in Romania. Julius-Kuhn-Archiv 427:342-346.

Zagrai L, Zagrai I, Levy L, Mavrodieva V, Festila A, Baias I (2011). A preliminary survey on Plum pox virus on cherry in Romania. Bulletin UASVM Horticulture 68(1):517. http://dx.doi.org/10.15835/buasvmcn-hort:7040

Zagrai I, Zagrai, L, Guzu G, Moldovan C, Roșu-Mareș S, Șofron A (2021). Virus infections in new plum orchards from Moldova, Romania. Scientific Papers. Series B, Horticulture LXV(1):292-296.

Published

2022-06-30

How to Cite

ZAGRAI, L. A., ZAGRAI, I., GUZU, G. M., ROȘU-MAREȘ, S. D., & MOLDOVAN, C. (2022). Assessment of the virus infections occurrence in new established plum and sweet cherry orchards in Transylvania, Romania. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(2), 12734. https://doi.org/10.15835/nbha50212734

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DOI: 10.15835/nbha50212734

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