The pivotal role of biochar in enhancement soil properties, morphophysiological and yield characters of barley plants under drought stress
Keywords:antioxidants, barley, biochar, drought, ; electrolyte leakage, lipid peroxidation
Drought is one of the most harmful abiotic stresses in arid and semiarid regions, so, field experiments were performed to examine biochar impact (15 or 20 t ha−1) on soil properties, physiological, morphological, and yield of barley under drought conditions. Our results displayed that drought caused a remarkable decrease in stem height and leaf area. Additionally, relative water contents (RWC%), chlorophyll a and b concentrations, as well as yield parameters were significantly reduced under drought. Conversely, lipid peroxidation (MDA), electrolyte leakage (EL%), and enzymatic activity were significantly augmented in the stressed plants during both seasons. Application of biochar led to improve leaves number (15.3), stem height (57%) and leaf area. Also, physiological characters like chlorophyll (72%) and RWC (33%), as well as yield, were increased considerably. Contrariwise, MDA and EL were reduced significantly (47 and 54%) under biochar application; furthermore, biochar led to regulate peroxidase and catalase activity in the stressed plants. It is concluded that biochar treatment can significantly improve soil properties, particularly soil EC (dSm-1), soil organic matter % and soil pH as well as increase yield characters via improving stress tolerance of barley under drought conditions; the best treatment was 20 t biochar ha−1 in the plants irrigated twice.
Abdelaal KhA, Omara IR, Hafez, YM, Esmail SM, EL Sabagh A (2018). Anatomical, biochemical and physiological changes in some Egyptian wheat cultivars inoculated with Puccinia graminis f.sp. tritici. Fresenius Environmental Bulletin 27:296-305.
Abdelaal KhA, Attia KA, Alamery SF, El-Afry M, Ghazy AI, Tantawy DS, … Hafez YM (2020a). Exogenous application of proline and salicylic acid can mitigate the injurious impacts of drought stress on barley plants associated with physiological and histological characters. Sustainability 12:1736. https://doi.org/10.3390/su12051736
Abdelaal KhA, EL-Maghraby LM, Elansary H, Hafez YM, Ibrahim EI, El-Banna M, … Elkelish A (2020b). Treatment of sweet pepper with stress tolerance-inducing compounds alleviates salinity stress oxidative damage by mediating the physio-biochemical activities and antioxidant systems. Agronomy 10:26. https://doi.org/10.3390/agronomy10010026
Abdelaal KhA, Attia KA, Niedbała G, Wojciechowski T, Hafez Y, Alamery S, Alateeq TK, Arafa SA (2021a). Mitigation of drought damages by exogenous chitosan and yeast extract with modulating the photosynthetic pigments, antioxidant defence system and improving the productivity of garlic plants. Horticulturae 7:510. https://doi.org/10.3390/horticulturae7110510
Abdelaal KhA, AlKahtani MDF, Attia K, Hafez Y, Király L, Künstler A (2021b). The role of plant growth-promoting bacteria in alleviating the adverse effects of drought on plants. Biology 10:520. https://doi.org/10.3390/biology10060520
Abdelaal KhA (2015). Effect of salicylic acid and abscisic acid on morpho-physiological and anatomical characters of faba bean plants (Vicia faba L.) under drought stress. Journal of Plant Production 6:1771-1788. https://doi.org/10.21608/JPP.2015.52096
Abdelaal KhA, Hafez YM, EL Sabagh A, Saneoka H (2017). Ameliorative effects of abscisic acid and yeast on morpho-physiological and yield characteristics of maize plant (Zea mays L.) under drought conditions. Fresenius Environmental Bulletin 26:7372-7383.
Aebi HE (1983). Catalase. In: Methods of Enzymatic Analysis. 3rd ed., Verlag Chemie: Weinheim, Germany, pp 273-286.
Agegnehu G, Srivastava AK, Bird MI (2017). The role of biochar and biochar compost in improving soil quality and crop performance: A review. Applied Soil Ecology 119:156-170. https://doi.org/10.1016/j.apsoil.2017.06.00
Ali Q, Ali S, Iqbal N, Javed MT, Rizwan M, Khaliq R, … Alyemeni MN (2019). Alpha-tocopherol fertigation confers growth physio-biochemical and qualitative yield enhancement in field grown water deficit wheat (Triticum aestivum L.). Scientific Reports 9:12924. https://doi.org/10.1038/s41598-019-49481-7
Ali Q, Daud MK, Haider MZ, Ali S, Rizwan M, Aslam N, … Deeba F (2017). Seed priming by sodium nitroprusside improves salt tolerance in wheat (Triticum aestivum L.) by enhancing physiological and bio-chemical parameters. Plant Physiology and Biochemistry 119:50-58. https://doi.org/10.1016/j.plaphy.2017.08.010
Ali S, Rizwan M, Qayyum MF, Ok YS, Ibrahim M, Riaz M, … Shahzad AN (2017). Biochar soil amendment on alleviation of drought and salt stress in plants: A critical review. Environmental Science and Pollution Research 24:12700-12712. https://doi.org/10.1007/s11356-017-8904-x
AOAC. (2005). Official methods of analysis of AOAC International, Gaithersburg, Md.: AOAC International,2005. https://www.worldcat.org/title/official-methods-of-analysis-of-aoac-international/oclc/62751475
Arafa SA, Attia KA, Niedbała G, Piekutowska M, Alamery S, Abdelaal K, … Attallah SY (2021). Seed priming boost adaptation in pea plants under drought stress. Plants 10:2201. https://doi.org/10.3390/plants10102201
Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water stress studies. Plant Soil 39:205-207.
Boyer JS (1988). Cell enlargement and growth – induced water potentials. Physiologia Plantarum 73:311-316. https://doi.org/10.1111/j.1399-3054.1988.tb00603.x
Cerozi B, da S, Fitzsimmons K (2016). The effect of pH on phosphorus availability and speciation in an aquaponics nutrient solution. Bioresource Technology 219:778e781. https://doi.org/10.1016/j.biortech.2016.08.079
Chng HY, Ahmed OH, Majid NMA (2015). Improving phosphorus availability, nutrient uptake and dry matter production of Zea mays L. on a tropical acid soil using poultry manure biochar and pineapple leaves compost. Experimental Agriculture 52:447-465. https://doi.org/10.1017/S0014479715000204
Duncan BD (1955). Multiple ranges and multiple F-test. Biometrics 11:1-42.
Esmail SM, Omara RI, Abdelaal KhAA, Hafez M (2019). Histological and biochemical aspects of compatible and incompatible wheat-Puccinia striiformis interactions. Physiological and Molecular Plant Pathology 106:120-128.
Elkelish A, Qari SH, Mazrou Y, Abdelaal KhA, Hafez YM, Abu-Elsaoud AM, … El Nahhas N (2020). Exogenous ascorbic acid induced chilling tolerance in tomato plants through modulating metabolism, osmolytes, antioxidants, and transcriptional regulation of catalase and heat shock proteins. Plants 10:431. https://doi.org/10.3390/plants9040431
El Nahhas N, AlKahtani M, Abdelaal KhA, Al Husnain L, AlGwaiz H, Hafez YM, … Elkelish A (2021). Biochar and jasmonic acid application attenuates antioxidative systems and improves growth, physiology, nutrient uptake and productivity of faba bean (Vicia faba L.) irrigated with saline water. Plant Physiology and Biochemistry 166:807-817. https://doi.org/10.1016/j.plaphy.2021.06.033
El-Nashaar F, Hafez YM, Abdelaal KhA, Abdelfatah A, Badr M, El-Kady S, Yousef A (2020). Assessment of host reaction and yield losses of commercial barley cultivars to Drechslera teres the causal agent of net blotch disease in Egypt. Fresenius Environmental Bulletin 29:2371-2377.
EL-Sabagh A, Abdelaal KhAA, Barutcular C (2017). Impact of antioxidants supplementation on growth, yield and quality traits of canola (Brassica napus L.) under irrigation intervals in North Nile Delta of Egypt. Journal of Experimental Biology and Agricultural Sciences 5:163-172. https://doi.org/10.18006/2017.5(2).163.172
EL Sabagh A, Hossain A, Barutcular C, Islam MS, Awan SI, Galal A, … Saneoka H (2019). Wheat (Triticum aestivum L.) production under drought and heat stress-adverse effects, mechanisms and mitigation: A review. Applied Ecology and Environmental Research 17:8307-8332. http://dx.doi.org/10.15666/aeer/1704_83078332
Gomez KA, Gomez AA (1984). Statistical Procedures for Agricultural Research. 2nd ed., Wiley Inter Science: New York, NY, USA, pp 1-690.
Ghorbanpour M, Mohammadi H, Kariman K (2020). Nanosilicon-based recovery of barley (Hordeum vulgare) plants subjected to drought stress. Environmental Science: Nano 7:443-461. https://doi.org/10.1039/C9EN00973F
Hafez YM, Abdelaal KhAA, Eid ME, Mehiar FF (2016). Morpho-physiological and biochemical responses of barley plants (Hordeum vulgare L.) against barley net blotch disease with application of non-traditional compounds and fungicides. Egyptian Journal of Biological Pest Control 26:261-268.
Hafez YM, Mourad RY, Mansour M, Abdelaal KhA (2014). Impact of non-traditional compounds and fungicides on physiological and biochemical characters of barely infected with Blumeria graminis f. sp hordei under field conditions. Egyptian Journal of Biological Pest Control 24:445-453.
Hafez YM, Attia KA, Alamery S, Ghazy A, Al-Dosse A, Ibrahim E, … Abdelaal KhA (2020). Beneficial effects of biochar and chitosan on antioxidative capacity, osmolytes accumulation, and anatomical characters of water-stressed barley plants. Agronomy 10:630. https://doi.org/10.3390/Agronomy10050630
Hammerschmidt R, Nuckles EM, Kuć J (1982). Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium. Physiological Plant Pathology 20:73-82. https://doi.org/10.1016/0048-4059(82)90025-X
Haider G, Steffens D, Müller C, Kammann CI (2016). Standard extraction methods may underestimate nitrate stocks captured by field-aged biochar. Journal of Environmental Quality 45:1196e1204. https://doi.org/10.2134/jeq2015.10.0529
Hodges DM, DeLong JM, Forney CF, Prange RK (1999). Improving the thiobarbituric acid reactive substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604-611. https://doi.org/10.1007/s004250050524
IPCC (2014). Intergovernmental Panel on Climate Change, Climate change: impacts, adaptation, and vulnerability. Cambridge University Press, Cambridge. http://www.ipcc.ch/report/ar5/wg2/
Jackson ML (1976). Soil Chemical Analysis. Printice-Hall of India Private Limited, New Delhi, pp 498.
Jaiswal AK, Alkan N, Elad Y, Sela N, Philosoph AM, Graber ER, Frenkel O (2020). Molecular insights into biochar-mediated plant growth promotion and systemic resistance in tomato against Fusarium crown and root rot disease. Scientific Reports 10:13934. https://doi.org/10.1038/s41598-020-70882-6
Levitt J (1980). Responses of plant to environmental stress: water, radiation, salt and other stresses. Academic Press, New York.
Moran R, Porath D (1982). Chlorophyll determination in intact tissues using N,N-Dimethyl formamide. Plant Physiology 69:1370-1381.
Naeem A, Akhtar M, Ahmad W (2013). Optimizing available phosphorus in calcareous soils fertilized with diammonium phosphate and phosphoric acid using Freundlich adsorption isotherm. The Scientific World Journal. https://doi.org/10.1155/2013/680257
Ohno T, Zibilske LM (1991). Determination of low concentrations of phosphorus in soil extracts using malachite green. Soil Science Society of America Journal 55:892. https://doi.org/10.2136/sssaj1991.03615995005500030046x
Omara RI, El-Kot GA, Fadel FM, Abdelaal KhAA, Saleh EM (2019). Efficacy of certain bioagents on patho-physiological characters of wheat plants under wheat leaf rust stress. Physiological and Molecular Plant Pathology 106:102-108. https://doi.org/10.1016/j.pmpp.2018.12.010
Penn CJ, Camberato J (2019). A critical review on soil chemical processes that control how soil pH affects phosphorus availability to plants. Agricultural 9:120. https://doi.org/10.3390/agriculture9060120
Qayyum MF, Haider G, Iqbal M, Hameed S, Ahmad N, Zia ur Rehman M, … Ali S (2020). Effect of alkaline and chemically engineered biochar on soil properties and phosphorus bioavailability in maize, Chemosphere 266:128980. https://doi.org/10.1016/j.chemosphere.2020.128980
Rashwan EAA, Abdelaal KhA (2019). Effect of nano zink-oxide foliar application on some flax cultivars under different irrigation treatments. Egypt Journal of Plant Breeding 23:119-145.
Richards LA. (1954). Diagnosis and Improving of Saline and Alkaline Soils. U.S., Salinity Laboratory Staff. Agriculture Handbook. No 60.
Rafique M, Ortas I, Rizwan M, Chaudhary HJ, Gurmani AR, Munis MFH (2020). Residual effects of biochar and phosphorus on growth and nutrient accumulation by maize (Zea mays L.) amended with microbes in texturally different soils. Chemosphere 238:1-9. https://doi.org/10.1016/j.chemosphere.2019.124710
Rehman MZ, Batool Z, Ayub MA, Hussaini KM, Murtaza G, Usman M, … Ali S (2020). Effect of acidified biochar on bioaccumulation of cadmium (Cd) and rice growth in contaminated soil. Environmental Technology & Innovation 19:101015. https://doi.org/10.1016/j.eti.2020.101015
Samarah NH (2005). Effects of drought stress on growth and yield of barley. Agronomy for Sustainable Development 25:145-149.
Sanchez-Monedero MA, Cayuela ML, Roig A, Jindo K, Mondini C, Bolan N (2018). Role of biochar as an additive in organic waste composting. Bioresources Technology 247:1155-1164. https://doi.org/10.1016/j.biortech.2017.09.193
Sorrenti G, Masiello CA, Toselli M (2016). Biochar interferes with kiwifruit Fe-nutrition in calcareous soil. Geoderma 272:10-19. https://doi.org/10.1016/j.geoderma.2016.02.017
Szalai G, Janda T, Padi E, Szigeti Z (1996). Role of light in post-chilling symptoms in maize. Journal of Plant Physiology 148:378-383. https://doi.org/10.1016/S0176-1617(96)80269-0
Su X, Su X, Yang S, Zhou G, Ni M, Wang C, Qin H, Zhou X, Deng J (2020). Drought changed soil organic carbon composition and bacterial carbon metabolizing patterns in a subtropical evergreen forest. Science of the Total Environment 736:139568. https://doi.org/10.1016/j.scitotenv.2020.139568
Wang J, Song L, Gong X, Xu J, Li M (2020). Functions of jasmonic acid in plant regulation and response to abiotic stress. International Journal of Molecular Sciences 21:1446. https://doi.org/10.3390/ijms21041446
Wang R, Gao M, Ji S, Wang S, Meng Y, Zhou Z (2016). Carbon allocation, osmotic adjustment, antioxidant capacity and growth in cotton under long-term soil drought during flowering and boll-forming period. Plant Physiology and Biochemistry 107:137-146. https://doi.org/10.1016/j.plaphy.2016.05.035
Waqas M, Shahzad R, Hamayun M, Asaf S, Khan AL, Kang SM, Yun S, Kim KM, Lee IJ (2018). Biochar amendment changes jasmonic acid levels in two rice varieties and alters their resistance to herbivory. PloS One 13:e0191296. https:// doi.org/10.1371/journal.pone.0191296
Wei W, Yang H, Fan M, Chen H, Guo D, Cao J, Kuzyakov Y (2020). Biochar effects on crop yields and nitrogen loss depending on fertilization. Science of the Total Environment 702:134423. https://doi.org/10.1016/j.scitotenv.2019.134423
Yang X, Li Y, Ren B, Ding L, Gao C, Shen Q, Shiwei S (2012). Drought-induced root aerenchyma formation restricts water uptake in rice seedlings supplied with nitrate. Plant and Cell Physiology 53:495-504. https://doi.org/10.1093/pcp/pcs003
Zheng H, Wang Z, Deng X, Zhao J, Luo Y, Novak J, Herbert S, Xing B (2013). Characteristics and nutrient values of biochars produced from giant reed at different temperatures. Bioresources Technology 130:463-471. https://doi.org/10.1016/j.biortech.2012.12.044
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