A survey of ‘Candidatus Phytoplasma pyri’ isolates in the Czech Republic based on imp gene genotyping

  • Lucie VALENTOVÁ Research and Breeding Institute of Pomology Holovousy Ltd., Laboratory for Molecular Biology, Holovousy 129, 508 01, Hořice (CZ)
  • Markéta BOHUNICKÁ University of Hradec Králové, Department of Biology, Faculty of Science, Rokitanského 62, 500 03 Hradec Králové (CZ)
  • Martina REJLOVÁ Research and Breeding Institute of Pomology Holovousy Ltd., Laboratory for Molecular Biology, Holovousy 129, 508 01, Hořice (CZ)
  • Jana SUCHÁ Research and Breeding Institute of Pomology Holovousy Ltd., Laboratory for Molecular Biology, Holovousy 129, 508 01, Hořice; Central Institute for Supervising and Testing in Agriculture, Železnická, 5060 01, Jičín (CZ)
  • Tomáš NEČAS Mendel University in Brno, Faculty of Horticulture, Department of Pomology, Valtická 337, 691 44 Lednice (CZ)
  • Aleš EICHMEIER Mendel University in Brno, Faculty of Horticulture, Mendeleum - Institute of Genetics, Valtická 334, 691 44 Lednice (CZ)
  • Tomáš KISS Mendel University in Brno, Faculty of Horticulture, Department of Pomology, Valtická 337, 691 44 Lednice (CZ)
  • Jana CMEJLOVA Research and Breeding Institute of Pomology Holovousy Ltd., Laboratory for Molecular Biology, Holovousy 129, 508 01, Hořice (CZ)
  • Radek CMEJLA Research and Breeding Institute of Pomology Holovousy Ltd., Laboratory for Molecular Biology, Holovousy 129, 508 01, Hořice (CZ) https://orcid.org/0000-0002-0707-8850
Keywords: 16SrX group phytoplasma, genetic variability, immunodominant membrane protein, pear decline symptoms

Abstract

Candidatus Phytoplasma spp.’ are pathogenic bacteria that infect many plant species. ‘Candidatus Phytoplasma pyri’, one of the members of the 16SrX group causes pear decline disease that adversely affects pear crops. To describe the prevalence of ‘Ca. P. pyri’ genotypes in the Czech Republic, 143 pear samples were collected from 41 locations including commercial orchards as well as trees along roads. Phytoplasma was detected by PCR in 115 samples, and it was possible to determine imp gene genotype in 84 samples. The most frequent genotypes were A1, B1, and C, which were identified in 71% of phytoplasma positive samples. ‘Ca. P. pyri’ was present either alone or as a mix of two populations in 88% of genotyped samples, and in another 6% of samples it was found in a mixed infection with ‘Ca. Phytoplasma mali’. A sole infection with ‘Ca. Phytoplasma mali’ was observed in 6% of samples. As for symptoms, 19% of symptomatic samples were found to be phytoplasma negative, and 74% of asymptomatic samples proved to be phytoplasma positive; leaf roll was more often observed in phytoplasma positive samples, while leaf narrowing rather indicated the absence of phytoplasma. The mildest symptoms were observed in samples infected with ‘Ca. P. pyri’ of the A1 imp genotype.

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Published
2022-03-02
How to Cite
VALENTOVÁ, L., BOHUNICKÁ, M., REJLOVÁ, M., SUCHÁ, J., NEČAS, T., EICHMEIER, A., KISS, T., CMEJLOVA, J., & CMEJLA, R. (2022). A survey of ‘Candidatus Phytoplasma pyri’ isolates in the Czech Republic based on imp gene genotyping. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(1), 12602. https://doi.org/10.15835/nbha50112602
Section
Research Articles
CITATION
DOI: 10.15835/nbha50112602