Effects of organic and inorganic fertilization with bio-inoculants on the sustainable management of plant-parasitic nematodes infesting okra (Abelmoschus esculentus)


  • Safiuddin ANSARI Aligarh Muslim University, Department of Botany, Plant Pathology and Nematology Laboratory, Aligarh-202002 (IN)
  • Gufran AHMAD Aligarh Muslim University, Department of Botany, Section of Environmental Botany, Aligarh-202002 (IN)
  • Abeer ELHAKEM Prince Sattam Bin Abdulaziz University, Department of Biology, College of Sciences and Humanities, 11942 Al-Kharj (SA)
  • Rose RIZVI Aligarh Muslim University, Department of Botany, Plant Pathology and Nematology Laboratory, Aligarh-202002 (IN)
  • Sartaj A. TIYAGI Aligarh Muslim University, Department of Botany, Plant Pathology and Nematology Laboratory, Aligarh-202002 (IN)
  • Irshad MAHMOOD Aligarh Muslim University, Department of Botany, Plant Pathology and Nematology Laboratory, Aligarh-202002 (IN)
  • Heba I. MOHAMED Ain Shams University, Faculty of Education, Department of Biological and Geological Sciences, Cairo (EG)




Azospirillum, Azotobacter, management, neem-seed cake, plant-parasitic nematodes


DOI: 10.15835/nbha49412544

Root-knot nematodes are believed to be amongst the biological constraints causing severe damage and a great reduction in the productivity of okra. The purpose of this study was to apply organic matter and non-symbiotic nitrogen-fixing bacteria to minimize the addition of chemical fertilizers that constantly pollute the environment.  Experimental studies were conducted in the field for two summers to determine the effect of inoculations of non-symbiotic nitrogen-fixing bacteria, such as Azotobacter chroococcum and Azospirillum brasilense singly and in combinations, with different recommended doses of inorganic nitrogen as well as organic matter such as neem seed cake on the growth, yield, and organic parameters of the okra crop towards the management of plant-parasitic nematodes. The results show a significant reduction in nematode multiplication through soil application of nitrogen-fixing bacteria and neem seed cake along with different recommended doses of nitrogen inoculated plants. Azotobacter was found to be less effective than Azospirillum in agronomic parameters and nematode control. The most pronounced increases were observed in the yield and growth parameters such as plant height, fresh as well as dry weights, fruit weights/plant, number of total fruits/plant and primary branches, chlorophyll content, and ascorbic acid content when A. chroococcum and A. brasilense were added concomitantly in various combinations. Agronomic parameters such as NPK content in the plant as well as in residual soil increased considerably in almost all the combinations irrespective of these biofertilizers and neem seed cake. In conclusion, the combined application of a 100% recommended dose of nitrogen fertilizer along with Azospirillum and neem seed cake is recommended for better growth and yield of okra with better control of nematodal population.


Aasfar A, Bargaz A, Yaakoubi K, Hilali A, Bennis I, Zeroual Y, Meftah Kadmiri I (2021). Nitrogen fixing Azotobacter species as potential soil biological enhancers for crop nutrition and yield stability. Frontiers in Microbiology 12:628379. https://doi.org/10.3389/fmicb.2021.628379

Abd El-Aal MMM, Salem AA (2018). Ameliorating growth performance and active compounds of moringa plant by integrated nutrients management. Journal of Plant Production Mansoura University 9(3):259-268

Abdel-Sattar M, Haikal AM, Hammad SE (2020). Meloidogyne incognita population control and nutritional status and productivity of Thompson seedless grapevines managed with different treatments. PLoS One 15(10):e0239993. https://doi.org/10.1371/journal.pone.0239993

Abd El- Rahman SS, Mohamed HI (2014) Application of benzothiadiazole and Trichoderma harzianum to control faba bean chocolate spot disease and their effect on some physiological and biochemical traits. Acta Physiologia Plantarum 36(2): 343-354. https://doi.org/10.1007/s11738-013-1416-5

Abu-Shahba MS, Mansour MM, Mohamed HI, Sofy MR (2021). Comparative cultivation and biochemical analysis of iceberg lettuce grown in sand soil and hydroponics with or without microbubble and microbubble. Journal of Soil Science and Plant Nutrition 21:389-403.

Ahmad G, Khan A, Khan AA, Ali A, Mohamed HI (2021a). Biological control: a novel strategy for the control of the plant parasitic nematodes. Antonie van Leeuwenhoek 114:885-912. https://doi.org/10.1007/s10482-021-01577-9

Ahmad G, Khan AA, Mohamed HI (2021b). Impact of the low and high concentrations of fly ash amended soil on growth, physiological response and yield of pumpkin (Cucurbita moschata Duch. Ex Poiret L.). Environmental Science and Pollution Research 28:17068-17083. https://doi.org/10.1007/s11356-020-12029-8

Akram M, Rizvi R, Sumbul A, Ansari RA, Mahmood I (2016). Potential role of bioinoculants and organic matter for the management of root-knot nematode infesting chickpea. Cogent Food Agriculture 2(1):1183457. https://doi.org/10.1080/23311932.2016.1183457

Aly AA, Mansour MTM, Mohamed HI, Abd-Elsalam KA (2012). Examination of correlations between several biochemical components and powdery mildew resistance of flax cultivars. Plant Pathology Journal 28(2):149-155.

Anand Ballal, Kadam AS (2016). Okra crop growth and yield responses to different organic sources of nitrogen. International Journal of Agricultural Science 8(48):2042-2044.

Ashry NA, Ghonaim MM, Mohamed HI, Mogazy AM (2018). Physiological and molecular genetic studies on two elicitors for improving the tolerance of six Egyptian soybean cultivars to cotton leaf worm. Plant Physiology and Biochemistry 130:224-234. https://doi.org/10.1016/j.plaphy.2018.07.010

Arora M, Saxena P, Abdin MZ, Varma A (2018). Interaction between Piriformospora indica and Azotobacter chroococcum governs better plant physiological and biochemical parameters in Artemisia annua L. plants grown under in vitro conditions. Symbiosis 75:103-112. https://doi.org/10.1007/s13199-017- 0519-y

Ayito EO, Iren OB (2018). Effects of rates of NPK, neem-based organic fertilizer and their combinations on okra (Abelmoschus esculentus L. Moench) yield and leaf nutrient content in south-eastern Nigeria. World News Natural Science 20:196-207.

Bahadur A, Singh J, Singh KP, Upadhyay AK, Rai M (2009). Morpho-physiological, yield and quality traits in lettuce (Lactuca sativa) as influenced by use of organic manures and biofertilizers. Indian Journal of Agriculture Science 79:282-285.

Bairwa HL, Shukla AK, Mahawer LN, Kaushik RA, Shukla KB, Ameta KD (2009). Response of integrated nutrient management on yield, quality and physico-chemical characteristics of okra cv. Arka Anamika. Indian Journal of Horticulture 66(3):310-314.

Bhattacharya S, Nanda MK, Mukherjee AK (2004). Effect of phosphorus and cutting on biomass and seed production of Stylosanthes cultivars. Forage Research 30:96-98.

Cassan F, Coniglio A, López G, Molina R, Nievas S, Le Noir de Carlan C, … Mora V (2020). Everything you must know about Azospirillum and its impact on agriculture and beyond. Biology and Fertility of Soils 56:461-479. https://doi.org/10.1007/s00374-020-01463-y

Chattopadhyay P, Banerjee G, Mukherjee S (2017). Recent trends of modern bacterial insecticides for pest control practice in integrated crop management system. 3 Biotech 7:60. https://doi.org/10.1007/s13205-017-0717-6

Chauhan A, Guleria S, Balgir PP, Walia A, Mahajan R, Mehta P, Shirkot CK (2017). Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect. Brazilian Journal of Microbiology 48(2):294-304. https://doi.org/10.1016/j.bjm.2016.12.001

Choudhary S, Yamini, Raheja N, Yadav SK, Kamboj ML, Sharma A (2018). A review: Pesticide residue: Cause of many animal health problems. Journal of Entomology and Zoology Studies 6(3):330-333.

Choudhary S, Pareek S, Saxena J (2010). Management of dry root rot of green gram (Vigna radiata) caused by Macrophomina phaseolina. Indian Journal of Agriculture Science 80:988-992.

Datta JK, Banerjee A, Saha Sikdar M, Gupta S, Mondal NK (2009). Impact of combined exposure of chemical fertilizer, biofertilizer and compost on growth, physiology and productivity of Brassica campestries in old alluvial soil. Journal of Environmental Biology 30:797-800.

Eifediyi EK, Mohammed KO, Remison SU (2015). Effects of neem (Azadirachta indica L.) seed cake on the growth and yield of okra (Abelmoschus esculentus (L.) Moench). Poljoprivreda/Agriculture 21:46-52. https://doi.org/10.18047/poljo.21.1.8

El-Beltagi HS, Mohamed HI, Elmelegy AA, Eldesoky SE, Safwat G (2019). Phytochemical screening, antimicrobial, antioxidant, anticancer activities and nutritional values of cactus (Opuntia Ficus Indicia) pulp and peel. Fresenius Environmental Bulletin 28(2A):1534-1551.

El-Beltagi HS, Sofy MR, Aldaej MI, Mohamed HI (2020). Silicon alleviates copper toxicity in flax plants by up-regulating antioxidant defense and secondary metabolites and decreasing oxidative damage. Sustainability 12:4732. https://doi.org/10.3390/su12114732

El-Hadad ME, Mustafa MI, Selim SM, El-Tayeb TS, Mahgoob AEA, Abdel Aziz NHA (2011). The nematicidal effect of some bacterial biofertilizers on Meloidogyne incognita in sandy soil. Brazilian Journal of Microbiology 42:105-113.

El-Nagdi WMA, Youssef MMA, El-Khair HA, Abd-Elgawad MMM (2019). Effect of certain organic amendments and Trichoderma species on the root-knot nematode, Meloidogyne incognita, infecting pea (Pisum sativum L.) plants. Egyptian Journal of Biological Pest Control 29(75):1-9. https://doi.org/10.1186/s41938-019-0182-0

El-Sheshtawy HS, Sofy MR, Ghareeb DA, Yacout GA, Eldemellawy MA, Ibrahim BM (2021). Eco-friendly polyurethane acrylate (PUA)/natural filler-based composite as an antifouling product for marine coating. Applied Microbiology and Biotechnology 105(18):7023-7034. https://doi.org/10.1007/s00253-021-11501-w

Elnasikh MH, Osman AG, Sherif AM (2011). Impact of neem seed cake on soil microflora and some soil properties. Journal of Science & Technology 12:144-150.

Eroa MG (2015). Production and characterization of organic fertilizer from Tubang-Bakod (Jatropha Curcas) seed cake and chicken manure. Asia Pacific Journal of Multidiscip Research 3:9-13.

Fasusi OA, Cruz C, Babalola OO (2021). Agricultural sustainability: microbial biofertilizers in rhizosphere management. Agriculture 11:163. https://doi.org/10.3390/agriculture11020163

Gao C, El-Sawah AM, Ali DFI., Hamoud YA, Shaghaleh H, Sheteiwy MS (2020). The integration of bio and organic fertilizers improve plant growth, grain yield, quality and metabolism of hybrid maize (Zea mays L.). Agronomy 10: 319. https://doi.org/10.3390/agronomy10030319

Ghuge MB, Lekhi R, Karcho S, Kumar A (2015). Influence of integrated nutrient management on growth and seed yield of okra (Abelmoschus esculentus L. Monech) cv. VRO-6. Environment and Ecology 83(3):1073-1076.

Gondwe RL, Kinoshita R, Suminoe T, Aiuchi D, Palta JP, Tani M (2020). Available soil nutrients and npk application impacts on yield, quality, and nutrient composition of potatoes growing during the main season in Japan. American Journal of Potato Research 97:234-245. https://doi.org/10.1007/s12230-020-09776-2

Helmi A, Mohamed HI (2016). Biochemical and ulturasturctural changes of some tomato cultivars to infestation with Aphis gossypii Glover (Hemiptera: Aphididae) at Qalyubiya, Egypt. Gesunde Pflanzen 68:41-50. https://doi.org/10.1007/s10343-016-0361-9

Hiscox JD, Israelstam GF (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57:1332-1334. https://doi.org/10.1139/b79-163

Hussain MA, Mukhtar T (2019). Root-knot nematodes infecting okra in major vegetable growing districts of Punjab, Pakistan. Pakistan Journal of Zoology 51(3):1137-1143. https://doi.org/10.17582/journal.pjz/2019.51.3.1137.1143

IITA (1975). Annual Report for 1974. International Institute for Tropical Agriculture, Ibadan, Nigeria.

Jackson ML (1973). Soil chemical analysis. Prentice Hall of India Pvt. Ltd., New Delhi.

Kaur H, Mohan C (2013). Management of post flowering stalk rot of maize (Zea mays) caused by Fusarium moniliforme with native biocontrol agents. Indian Journal of Agriculture Science 83:1165-1172.

Kamran MA, Jiang, J, Li J, Shi R, Mehmood K, Baquy MAA, Xu R (2018). Amelioration of soil acidity, Olsen-P, and phosphatase activity by manure- and peat-derived biochars in different acidic soils. Arabian Journal of Geosciences 11:272.

Khalil MS (2013). Abamectin and azadirachtin as eco-friendly promising biorational tools in integrated nematodes management programs. Journal of Plant Pathology and Microbiology 4:4. https://doi.org/10.4172/2157-7471.1000174

Khan GA, Muhammad MQ, Muhamma J, Hussain TF (2009). Nutrient uptake, growth and yield of wheat (Triticum aestivum) as affected by zinc application rates. International Journal of Agriculture and Biology 11:389-396.

Khan Z, Tiyagi SA, Mahmood I, Rizvi R (2012). Effects of N fertilisation, organic matter, and biofertilisers on the growth and yield of chilli in relation to management of plant-parasitic nematodes. Turkish Journal of Botany 36:73-81. https://doi.org/10.3906/bot-1009-60

Khan AU, Ullah F, Khan N, Mehmood S, Fahad S, Datta R, … Hussain GS (2021). Production of organic fertilizers from rocket seed (Eruca sativa L.), chicken peat and Moringa Oleifera Leaves for growing linseed under water deficit stress. Sustainability 13: 59. https://doi.org/10.3390/su13010059

Kumar S, Verma SK, Singh K, Singh S (2011). Effect of nitrogen and sulphur on growth, yield and nutrient uptake by indian mustard (Brassica juncea) under rainfed condition. Indian Journal of Agriculture Science 81:145-149.

Kumar N, Singh HK, Mishra PK (2015). Impact of organic manures and biofertilizers on growth and quality parameters of strawberry cv. Chandler. Indian Journal of Science and Technology 8(15):51107. https://doi.org/10.17485/ijst/2015/v8i15/51107

Kumar V, Saikia Jumi, Nath DJ (2017). Effect of integrated nutrient management on growth, yield and quality of okra (Abelmoschus esculentus (L). Moench cv. Arka Anamika. International Journal of Chemical Studies 5(5):2001-2003.

Laishram N, Dhiman SR, Gupta YC, Bhardwaj SK, Singh A (2013). Microbial dynamics and physic-chemical properties of soil in the rhizosphere of chrysanthemum (Dendranthema grandiflora) as influenced by integrated nutrient management. Indian Journal of Agricultural Sciences 83:447-455.

Laxminarayan K, Patiram (2006). Effect of integrated use of inorganic, biological and organic manures on rice productivity and soil fertility in ultisols of Mizoram. Journal of the Indian Society of Soil Science 54:213-220.

Lindner RC (1944). Rapid analytical methods for some of the more common inorganic constituents of plant tissues. Plant Physiology 19:76-89. https://doi.org/10.1104/pp.19.1.76

Mamia A, Amin A, Roy TS, Faruk GM (2018). Influence of inorganic and organic fertilizers on growth and yield of soybean. Bangladesh Agronomy Journal 21:77-81. https://doi.org/10.3329/baj.v21i1.39363

Manunatha TG, Rai AB, Singh B (2017). Root-knot nematode: A threat to vegetable production and its management. IIVR Technical bulletin No. 76, IIVR, Varanasi, pp 32.

Meena RS, Kumar S, Datta R, Lal R, Vijayakumar V, Brtnicky M, … Marfo TD (2020). Impact of Agrochemicals on soil microbiota and management: A review. Land 9:34. https://doi.org/10.3390/land9020034

Mina BL, Saha S, Kumar N, Srivastava AK, Gupta HS (2008). Changes in soil nutrient content and enzymatic activity under conventional and zero-tillage practices in an Indian sandy clay loam soil. Nutrient Cycling in Agroecosystems 82:273-281. https://doi.org/10.1007/s10705-008-9189-8

Mishra B, Sahu GS, Tripathy P, Mohanty S, Pradhan B (2020). Impact of organic and inorganic fertilizers on growth, yield, nutrient uptake and soil fertility in okra (Abelmoschus esculentus (L.) Moench) cv. Pusa A-4. The Pharma Innovation Journal 9(7):210-213.

Mohamed HI (2011). Molecular and biochemical studies on the effect of gamma rays on lead toxicity in cowpea (Vigna sinensis) plants. Biological Trace Element Research 144:1205-1218. https://doi.org/10.1007/s12011-011-9058-1

Mohamed HI, Abd–El Hameed AG (2014). Molecular and biochemical markers of some Vicia faba L. genotype in response to storage insect pests infestation. Journal of Plant Interactions 9(1):618-626. https://doi.org/10.1080/17429145.2013.879678

Mohamed HI, Elsherbiny EA, Abdelhamid MT (2016). Physiological and biochemical responses of Vicia faba plants to foliar application with zinc and iron. Gesunde Pflanzen 68:201-212.

Mohamed HI, El-Beltagi HS, Aly AA, Latif HH (2018a). The role of systemic and non systemic fungicides on the physiological and biochemical parameters in Gossypium hirsutum plant, implications for defense responses. Fresenius Environmental Bulletin 27(12):8585-8593.

Mohamed HI, Akladious SA, El-Beltagi HS (2018b). Mitigation the harmful effect of salt stress on physiological, biochemical and anatomical traits by foliar spray with trehalose on wheat cultivars. Fresenius Environmental Bulletin 27(10):7054-7065.

Moustafa-Farag M, Mohamed HI, Mahmoud A, Elkelish A, Misra AN, Guy KM, … Zhang M (2020). Salicylic acid stimulates antioxidant defense and osmolyte metabolism to alleviate oxidative stress in watermelons under excess boron. Plants 9:724. https://doi.org/10.3390/plants9060724

Mukhtar T, Arshad I, Kayani MZ, Hussain MA, Kayani SB, Rahoo AM, Ashfaq M (2013). Estimation of damage to okra (Abelmoschus esculentus) by root-knot disease incited by Meloidogyne incognita. Pakistan Journal of Botany 45:1023-1027.

NHB (2018). National Horticulture Board.

Ntalli N, Adamski Z, Doula M, Monokrousos N (2020). Nematicidal amendments and soil remediation. Plants 9:429. https://doi.org/10.3390/plants9040429

Nosheen S, Ajmal, I, Song Y (2021). Microbes as biofertilizers, a potential approach for sustainable crop production. Sustainability 13:1868. https://doi.org/10.3390/su13041868

Oka Y (2010). Mechanisms of nematode suppression by organic soil amendments- A review. Applied Soil Ecology 44:101-115. https://doi.org/10.1016/j.apsoil.2009.11.003

Olabiyi TI, Ayeni BP (2015). Assessment of Azadirachta indica and Cleome viscose liquid-formulations as bio-nematicides in the management of nematode pests of okra. African Journal of Agriculture Research 1(6):467-471. https://doi.org/10.5897/AJAR2015.10163

Olsen SR, Cole CV, Watanabe FS, Dean LA (1954). Estimation of available phosphorus in soil by extraction with sodium bicarbonate. USDA Circular No. 939, Washington.

Oosterhuis D, Loka D, Kawakami E, Pettigrew W (2014). The physiology of potassium in crop production. Advances in Agronomy 126:203-234. https://doi.org/10.1016/B978-0-12-800132-5.00003-1

Prasad RK, Kumar V, Prasad B, Singh AP (2010). Long term effect of crop residues and zinc fertilizer on crop yield, nutrient uptake and fertility build up under rice-wheat cropping system in calciorthents. Journal of Inidan Socitey and Soil Science 58:205-211.

Prajapati K, Yami KD, Singh A (2008). Plant growth promotional effect of Azotobacter chroococcum, Piriformospora indica and vermicompost on rice plant. Nepal Journal of Science and Technology 9:85-90. https://doi.org/10.3126/njst.v9i0.3170

Rangana S (1976). Handbook of analysis and quality for fruit and vegetable products. Tata McGraw-Hill Publishing Co. Ltd, New Delhi, pp 1122.

Reza MS, Islam AKMS, Rahman MA, Miah MY, Akhter S, Rahman MM (2014). Impact of organic fertilizers on yield and nutrient uptake of cabbage (Brassica oleracea var. capitata). Journal of Science, Technology and Environment Informatics 03(02):231-244. https://doi.org/10.18801/jstei.030216.26

Rizvi R, Singh G, Safiuddin, Ansari RA, Tiyagi SA, Mahmood I (2015). Sustainable management of root-knot disease of tomato by neem cake and Glomus fasciculatum. Cogent Food Agriculture 1:1. https://doi.org/10.1080/23311932.2015.1008859

Roper MM, Gupta VVSR (2016.) Enhancing non-symbiotic N fixation in agriculture. The open Agriculture Journal 10:7-27. https://doi.org/10.2174/1874331501610010007

Sachan S, Singh D, Kasera S, Mishra SK, Tripathi Y, Mishra V, Singh RK (2017). Integrated nutrient management (INM) in Okra (Abelmoschus esculentus (L.) Moench) for better growth and higher yield. Journal of Pharmacognosy and Phytochemistry 6(5):1854-1856.

Safiuddin, Shahab S, Sharma S (2011). Pathogenicity of root-knot nematode, Meloidogyne incognita and root-rot fungus, Rhizoctonia solani on okra [Abelmoschus esculentus L.]. Journal of Electronic Science and Technology 3:97-102.

Santos HC, Pereira EM, de Medeiros RLS, Costa PMDA, Pereira WE (2019). Production and quality of okra produced with mineral and organic fertilization. Brazilian Journal of Environmental Sciences 23(2):97-102. https://doi.org/10.1590/1807-1929/agriambi.v23n2p97-102

Sen HS (2003). Problem soils in India and their management: Prospect and retrospect. Journal of the Indian Society of Soil Science 51:388-408.

Sharaf AEM, Farghal II, Sofy MR (2009). Response of broad bean and lupin plants to foliar treatment with boron and zinc. Australian Journal of Basic and Applied Science 3:2226-2231.

Singh KP, Suman A, Singh PN, Srivastava TK (2007). Improving quality of sugarcane-growing soils by organic amendments under subtropical climatic conditions of India. Biology and Fertility of Soils 44:367-376. https://doi.org/10.1007/s00374-007-0216-8

Singh S, Ram RB (2018). Biochemical study on Kashi Pragati of okra cultivar in respect with organic, inorganic and biofertilizers. Current Journal of Applied Science and Technology 27(5):1-5. https://doi.org/10.9734/CJAST/2018/41911

Sofy MR, Mohamed HI, Dawood MFA, Abu-Elsaoud AM, Soliman MH (2021a). Integrated usage of arbuscular mycorrhizal fungi and chicken waste biochar as economic potential tools to ameliorate antioxidant activity, osmolyte accumulation and salt endogenous hormone-stressed spinach plants. Archives of Agronomy and Soil Science. https://doi.org/10.1080/03650340.2021.1949709

Sofy AR, Sofy MR, Hmed AA, Dawoud RA, Alnaggar AE-AM, Soliman AM, El-Dougdoug NK (2021b). Ameliorating the adverse effects of tomato mosaic tobamovirus infecting tomato plants in Egypt by boosting immunity in tomato plants using zinc oxide nanoparticles. Molecules 26(5):1337. https://doi.org/10.3390/molecules26051337

Sofy MR, Aboseidah AA, Heneidak SA, Ahmed HR (2021c). ACC deaminase containing endophytic bacteria ameliorate salt stress in Pisum sativum through reduced oxidative damage and induction of antioxidative defense systems. Environmental Science and Pollution Research 28(30):40971-40991. https://doi.org/10.1007/s11356-021-13585-3

Sofy AR, Sofy MR, Hmed AA, Dawoud RA, Refaey EE, Mohamed HI, El-Dougdoug NK (2021d). Molecular characterization of the Alfalfa mosaic virus infecting Solanum melongena in Egypt and control of its deleterious effects with melatonin and salicylic acid. Plants 10(3):459. https://doi.org/10.3390/plants10030459

Soliman ASh, Shawky SM, Omar MNA (2011). Efficiency of bioagents in controlling root-knot nematode on Acacia plants in Egypt. American-Eurasian Journal of Agricultural & Environmental Sciences 10:223-229.

Southey JF (1986). Laboratory methods for work with plant and soil nematodes. Her Majesty’s Stationery Office, London, UK, pp 202.

Taba S, Tomoyose M, Moromizato Z (2006). Control mechanism associated with rice bran-amended soil for root-knot nematode, Meloidogyne incognita. International Journal of Nematology 16:126-133.

Timsina J (2018). Can organic sources of nutrients increase crop yields to meet global food demand? Agronomy 8:214. https://doi.org/10.3390/agronomy8100214

Tully KL, McAskill C (2020). Promoting soil health in organically managed systems: a review. Organic Agriculture 10:339-358. https://doi.org/10.1007/s13165-019-00275-1

Ullah MA, Aamir SS, Haider H, Adil B (2018). Growth of olive varieties in tunnel under salinity plus humic acid, biozote and vermicompost. International Journal of Advanced Research in Biological Sciences 5:118-124.

Velmourougane K, Prasanna R, Chawla G, Nain L, Kumar A, Saxena AK (2019). Trichoderma–Azotobacter biofilm inoculation improves soil nutrient availability and plant growth in wheat and cotton. Journal of Basic Microbiology 59:632-644. https://doi.org/10.1002/jobm.201900009

Verma OP, Paul S, Rathi MS (2000). Synergistic effect of coinoculation of Azotobacter chroococcum and Rhizobium on pea (Pisum sativum). Annals of Agricultural Sciences 21:418-420.

Wahane MR, Meshram NA, More SS, Khobragade NH (2020). Biofertilizer and their role in sustainable agriculture-A review. The Pharma Innovation Journal 9(7):127-130.

Wani S, Ali T (2013). Potential use of Azotobacter chroococcum in crop production: an overview. Current Agriculture Research Journal 1:35-38. http://dx.doi.org/10.12944/CARJ.1.1.04

Wani SA, Chand S, Wani MA, Ramzan M, Hakeem KR (2016). Azotobacter chroococcum – a potential biofertilizer in agriculture: an overview. Soil Science: Agricultural and Environmental Prospectives 333-348. https://doi.org/10.1007/978-3-319-34451-5_15

Youssef MMA, Eissa MFM (2014). Biofertilizers and their role in management of plant parasitic nematodes. A review. E3 Journal of Biotechnology and Pharmaceutical Research 5:1-6.

Zhang SR, Yue Qiang C, XinPing S, QinPing Z, Fusuo R, ChunQin VZ (2010). Influence of long-term nitrogen fertilization on micronutrient density in grain of winter wheat (Triticum aestivum L.). Journal of Cereal Science 51:165-170. https://doi.org/10.1016/j.jcs.2009.11.008

Zhu N, Tan X, Li M, Pan X, Shi Q (2018). Effects of different organic fertilizers on growth of rice seedlings raised in straw substrates. Acta Agriculturae Universitatis Jiangxiensis 40:286-294.




How to Cite

ANSARI, S. ., AHMAD, G. ., ELHAKEM, A. ., RIZVI, R. ., TIYAGI, S. A. ., MAHMOOD, I. ., & MOHAMED, H. I. . (2021). Effects of organic and inorganic fertilization with bio-inoculants on the sustainable management of plant-parasitic nematodes infesting okra (Abelmoschus esculentus). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(4), 12544. https://doi.org/10.15835/nbha49412544



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DOI: 10.15835/nbha49412544

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