Effect of gibberellin, nano-nutrition with titanium, zinc and iron on yield and some physiological and qualitative traits of white beans


  • Seyed Mostafa AZIMI Lorestan University, Iran, Department of Plant Production and Genetic Engineering (IR)
  • Hamid Reza EISVAND Lorestan University, Iran, Department of Plant Production and Genetic Engineering (IR) https://orcid.org/0000-0001-9751-9121
  • Ahmad ISMAILI Lorestan University, Iran, Department of Plant Production and Genetic Engineering (IR)
  • Naser AKBARI Lorestan University, Iran, Department of Plant Production and Genetic Engineering (IR)




chlorophyll, grain yield, nano-nutrition, nano-titanium, nano-zn


Plant nutrition has a vital role in crop production. This study was performed to investigate the effects of different application methods of some nutrients (nano Fe, Zn, and Ti), and gibberellin on yield, some morphophysiological and grain protein of white beans in 2018 as a factorial experiment in a randomized complete block design with four replications. Experimental factors included seed priming (hydropriming, gibberellin priming, titanium nano dioxide, and nano-Zn priming) and micronutrient foliar spraying (zinc, iron, and zinc + iron). The results illustrated that seed priming and foliar application significantly affected yield, yield components and chlorophyll content. Plant height increased in seed priming treatment with gibberellin and foliar application of zinc + iron by 13% compared to the control. Furthermore, this treatment enhanced the number of sub-branches per plant by 32% compared to the control. Grain yield components such as the number of pods per plant and 100-grain weight were also affected by seed priming with nano-Zn, and the simultaneous spraying of iron and zinc that grain yield by 18%, so that grain yield by 2649 kg ha–1 in hydropriming treatment reached to 3211 kg ha–1 in nano-Zn priming with simultaneous application of zinc and iron. Nano-Zn priming with iron foliar application caused the highest biological yield (9011 kg ha–1), which increased by 19% compared to control. Nano-Zn priming increased grain protein percentage by 21%. This treatment along with the foliar application of zinc + iron, significantly enhanced leaf chlorophyll content compared to other treatments. Therefore, to increase the yield of white beans, priming treatment with nano-Zn as well as foliar application of zinc + iron can be used.


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Alpana P, Priyankar, R., Akhila Nand D, Brijmohan P (2019). Effect of nano-titanium dioxide polymorphs priming on seed germination and seedling growth of French bean (Phaseolus vulgaris L.). International Journal of Agriculture, Environment and Biotechnology 12(2):121-127. https://doi.org/10.30954/0974-1712.06.2019.7

Arnon DI (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24(1):1-15. https://doi.org/10.1104/pp.24.1.1

Ashraf M, Foolad MR (2005). Pre‐sowing seed treatment-a shotgun approach to improve germination, plant growth, and crop yield under saline and non‐saline conditions. In: Advances in Agronomy, Academic Press, pp 223-271.

Bank L (1982). Effects of timing of foliar zinc fertilizer on yield components of soyabeans. Australian Journal of Experimental Agriculture 22(116):226-231. https://doi.org/10.1071/EA9820226

Baybordi A (2006). Zinc in soils and crop nutrition. First Ed., Parivar Press.

Bewley JD, Bradford KJ, Hilhorst HWM, Nonogaki H (2013). Seeds physiology of development, germination and dormancy. Third Ed., Springer, New York, Heidelberg, Dordrecht, London.

Bhattacharjee S, Mukherjee A (2002). Salt stress-induced cytosolute accumulation, antioxidant response and membrane deterioration in three rice cultivars during early germination. Seed Science and Technology 30:279-287.

Brear EM, Day DA, Smith PMC (2013). Iron: an essential micronutrient for the legume-rhizobium symbiosis. Frontiers in Plant Science 4:359-359. https://doi.org/10.3389/fpls.2013.00359

Dhoke SK, Mahajan P, Kamble R, Khanna A (2013). Effect of nanoparticles suspension on the growth of mung (Vigna radiata) seedlings by foliar spray method. Nanotechnology Development 3(1):e1. https://doi.org/10.4081/nd.2013.e1

do Espirito Santo Pereira A, Caixeta Oliveira H, Fernandes Fraceto L, Santaella C (2021). Nanotechnology potential in seed priming for sustainable agriculture. Nanomaterials 11(2):267. https://doi.org/10.3390/nano11020267

Eisvand HR, Kamaei H, Nazarian F (2018). Chlorophyll fluorescence, yield and yield components of bread wheat affected by phosphate bio-fertilizer, zinc and boron under late-season heat stress. Photosynthetica 56(4):1287-1296. https://doi.org/10.1007/s11099-018-0829-1

FAO (2019). FAOSTAT. https://www.fao.org/faostat/en/#data

Farhoudi R, Sharifzadeh F (2006). The effects of NaCl priming on salt tolerance in canola (Brassica napus L.) seedlings grown under saline conditions. Indian Journal of Crop Science 1:74-78.

Gorczyca A, Pociecha E, Kasprowicz M, Niemiec M (2015). Effect of nanosilver in wheat seedlings and Fusarium culmorum culture systems. European Journal of Plant Pathology 142(2):251-261. https://doi.org/10.1007/s10658-015-0608-9

Hajikhani S, Habibi H, Shekari F, Fotoukian MH (2011). The effect of seed priming on grain yield and its components of spotted bean cultivars under water deficit stress. Iranian Journal of Field Crop Science 42(1):191-197.

Hasanuzzaman M, Fotopoulos V (2019). Priming and pretreatments of seeds and seedlings: Implication in plant stress tolerance and enhancing productivity in crop plants. Springer, Singapore.

Itoh H, Tanaka-Ueguchi M, Kawaide H, Chen X, Kamiya Y, Matsuoka M (1999). The gene encoding tobacco gibberellin 3beta-hydroxylase is expressed at the site of GA action during stem elongation and flower organ development. Plant Journal 20(1):15-24. https://doi.org/10.1046/j.1365-313x.1999.00568.x

Jaberzadeh A, Moaveni P, Tohidi Moghadam HR, Zahedi H (2013). Influence of bulk and nanoparticles titanium foliar application on some agronomic traits, seed gluten and starch contents of wheat subjected to water deficit stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41(1):201-207. https://doi.org/10.15835/nbha4119093

Jalil Shesh Bahre M, Movahedi Dehnavi M (2012). Effect of zinc and iron foliar application on soybesn seed vigour grown under drought stress. Journal of Crop Production 5(1):19-35.

Jin Z, Minyan W, Lianghuan W, Jiangguo W, Chunhai S (2008). Impacts of combination of foliar iron and boron application on iron biofortification and nutritional quality of rice grain. Journal of Plant Nutrition 31(9):1599-1611. https://doi.org/10.1080/01904160802244803

Kiani S (2012). Effects of iron on efficiency and map of photosystem II photochemical yield of rose flower using chlorophyll fluorescence imaging. Journal of Soil and Plant Interactions 2(4):25-35.

Laware S, Raskar S (2014). Influence of zinc oxide nanoparticles on growth, flowering and seed productivity in onion. International Journal of Current Microbiology and Applied Sciences 3(7):874-881.

Lee S, Kim JH (2000). Total sugars, alpha-amylase activity, and germination after priming of normal and aged rice seeds. The Korean Journal of Crop Science 45:108-111.

Madadi M, Khomari S, Javadi A, Sofalian O (2016). Effect of black cumin seed priming with calcium nitrate and nano-zinc oxide on germinability and seedling growth under salinity stress. Journal of Plant Process and Function 5(15):169-180.

Mahmoodzadeh H, Nabavi M, Kashefi H (2013). Effect of nanoscale titanium dioxide particles on the germination and growth of canola (Brassica napus). Journal of Ornamental Plants 3(3):25-32.

Makarian H, Shojaei H, Damavandi A, Nasiri Dehsorkhi A, Akhyani A (2017). The effect of foliar application of zinc oxide in common and nanoparticles forms on some growth and quality traits of mungbean (Vigna radiata L.) under drought stress conditions. Iranian Journal Pulses Research 8(2):166-180. https://doi.org/10.22067/ijpr.v8i2.51644

Marschner P (2011). Marschner's mineral nutrition of higher plants. Academic Press, London, UK.

Mishra V, Mishra RK, Dikshit A, Pandey AC (2014). Interactions of nanoparticles with plants: An emerging prospective in the agriculture industry. In: Ahmad P, Rasool S (Eds). Emerging Technologies and Management of Crop Stress Tolerance. Academic Press, pp 592.

Mohamed Amanullah M, Archana J, Manoharan S, Subramanian KS (2012). Influence of iron and AM inoculation on metabolically active iron, chlorophyll content and yield of hybrid maize in calcareous soil. Journal of Agronomy 11:27-30. https://doi.org/10.3923/ja.2012.27.30

Morteza E, Moaveni P, Farahani HA, Kiyani M (2013). Study of photosynthetic pigments changes of maize (Zea mays L.) under nano TiO2 spraying at various growth stages. Springerplus 2(1):247. https://doi.org/10.1186/2193-1801-2-247

Nair R, Varghese SH, Nair BG, Maekawa T, Yoshida Y, Kumar DS (2010). Nanoparticulate material delivery to plants. Plant Science 179(3):154-163. https://doi.org/10.1016/j.plantsci.2010.04.012

Nazeri P, Kashani A, Khavazi K, Ardakani MR, Mirakhori M, Pour siah bidi M (2010). The effect of biofertilizer and phosphorus fertilizer banding with Zinc on white bean (Phaseolus vulgaris L.). Journal of Agroecology 2(1):175-185. https://doi.org/10.22067/jag.v2i1.7617

Pouryousef Miandoab, M, Esmaeilzadeh F (2017). The effect of foliar application of growth stimulants and priming on yield and grain oil content of flax (Linum usitatissimum L.). Journal of Crop Ecophysiology 10(4):874-857.

Prasad, TNVKV, Sudhakar P, Sreenivasulu Y, Latha P, Munaswamy V, Reddy KR, Sreeprasad TS, Sajanlal PR, Pradeep T (2012). Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. Journal of Plant Nutrition 35(6):905-927. https://doi.org/10.1080/01904167.2012.663443

Ravi S, Channel HT, Hebsur NS, Patil BN, Dharmatti PR (2008). Effect of sulphur, zinc and iron nutrition on growth, yield, nutrient uptake and quality of safflower (Carthamus tinctorius L.). Karnataka Journal of Agricultural Sciences 21(3):382-385.

Rengel Z (2001). Genotypic differences in micronutrient use efficiency in crops. Communications in Soil Science and Plant Analysis 32(7-8):1163-1186. https://doi.org/10.1081/CSS-100104107

Ru K, Hl S, Kunjadia B (2018). Effect of zinc and iron application on leaf chlorophyll, carotenoid, grain yield and quality of wheat in calcareous soil of Saurashtra region. International Journal of Chemical Studies 6:2092-2096.

Sartip H, Sirousmehr A (2017). Effect of titanium nano particles and different irrigation levels on photosynthetic pigments, proline, soluble carbohydrates and growth parameters of Purslane. Journal of Plant Ecophysiology 9(28):79-90.

Shafiee A, Sajedi N, Changizi M (2015). The effects of different treatments of seed priming and foliar application of nano particle and zinc sulphate on agronomic traits in safflower. Iranian Journal of Seed Science and Technology 4(2):71-80.

Shah T, Latif S, Saeed F, Ali I, Ullah S, Abdullah Alsahli A, Jan S, Ahmad P (2021). Seed priming with titanium dioxide nanoparticles enhances seed vigor, leaf water status, and antioxidant enzyme activities in maize (Zea mays L.) under salinity stress. Journal of King Saud University - Science 33(1):101207. https://doi.org/10.1016/j.jksus.2020.10.004

Sharifi R, Mohammadi K, Rokhzadi A (2016). Effect of seed priming and foliar application with micronutrients on quality of forage corn (Zea mays). Environmental and Experimental Biology 14(4):151-156. https://doi.org/10.22364/eeb.14.21

Singh A, Dahiru R, Musa M, Sani Haliru B (2014). Effect of osmopriming duration on germination, emergence, and early growth of cowpea (Vigna unguiculata L.) in the Sudan Savanna of Nigeria. International Journal of Agronomy 841238. https://doi.org/10.1155/2014/841238

Sytar O, Kumari P, Yadav S, Brestic M, Rastogi A (2019). Phytohormone priming: regulator for heavy metal stress in plants. Journal of Plant Growth Regulation 38(2):739-752. https://doi.org/10.1007/s00344-018-9886-8

Taiz L, Zeiger E, Moller I, Murphy A (2014). Plant physiology and development. 6 ed. Sinauer Associates, Sunderland, CT.

Tehrani MM, Malakouti MJ (2000). The role of micronutrients in increasing yield and improving the quality of agricultural products "Microelements with macro impact". Tarbiat Modares University, Iran.

Tymoszuk A, Wojnarowicz J (2020). Zinc oxide and zinc oxide nanoparticles impact on in vitro germination and seedling growth in Allium cepa L. Materials 13(12):2784. https://doi.org/10.3390/ma13122784

Würth B (2007). Emissions of engineered and unintentionally produced nanoparticles to the soil. an exposure assessment for Switzerland. ETH Zurich Department of Environmental Sciences.

Xiang L, Zhao HM, Li YW, Huang XP, Wu XL, Zhai T, Yuan Y, Cai QY, Mo CH (2015). Effects of the size and morphology of zinc oxide nanoparticles on the germination of Chinese cabbage seeds. Environmental Science and Pollution Research International 22(14):10452-10462. https://doi.org/10.1007/s11356-015-4172-9



How to Cite

AZIMI, S. M., EISVAND, H. R., ISMAILI, A., & AKBARI, N. (2022). Effect of gibberellin, nano-nutrition with titanium, zinc and iron on yield and some physiological and qualitative traits of white beans. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 50(1), 12538. https://doi.org/10.15835/nbha50112538



Research Articles
DOI: 10.15835/nbha50112538

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