Waterlogging tolerance evaluation of fifteen poplar clones cultivated in the Jianghan Plain of China


  • Ruonan GENG Huazhong Agricultural University, College of Horticulture and Forestry Sciences, Hubei Engineering Technology Research Center for Forestry Information, No. 1 Shizishan Street, Hongshan District, Wuhan (CN)
  • Xinye ZHANG Hubei Academy of Forestry, 370 Luoyu Road, Hongshan District, Wuhan (CN)
  • Xiaoping FAN Hubei Academy of Forestry, Shishou Poplar Research Institute, Nanyue Avenue, Shishou City, Jingzhou City (CN)
  • Qian HU Huazhong Agricultural University, College of Horticulture and Forestry Sciences, Hubei Engineering Technology Research Center for Forestry Information, No. 1 Shizishan Street, Hongshan District, Wuhan (CN)
  • Tianhong NI Huazhong Agricultural University, College of Horticulture and Forestry Sciences, Hubei Engineering Technology Research Center for Forestry Information, No. 1 Shizishan Street, Hongshan District, Wuhan (CN)
  • Kebing DU Huazhong Agricultural University, College of Horticulture and Forestry Sciences, Hubei Engineering Technology Research Center for Forestry Information, No. 1 Shizishan Street, Hongshan District, Wuhan (CN)




poplar, waterlogging stress, waterlogging tolerance


To provide references for poplar cultivation in waterlogged prone area of Jianghan Plain of China, the waterlogging tolerance of 15 poplar clones widely cultivated in these areas were evaluated based on their responses to 45-day waterlogging stress followed by 15-day drainage recovery in morphology, growth, biomass accumulation, leaf gas exchange and chlorophyll fluorescence parameters. The results showed that the normal watered seedlings (CK) of the 15 clones grew vigorously during the experiment, and no defoliation and death occurred. For the seedlings under waterlogging treatment (water 10 cm above the soil surface), its morphology changed markedly, including slowing growth, chlorosis and abscission of leaves, development of hypertrophied lenticels and adventitious roots etc. Waterlogging stress significantly inhibited the seedling growth of height and ground diameter, biomass accumulation, as well as leaf gas exchange and chlorophyll fluorescence parameters of the 15 clones with varying degrees. The net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), intercellular CO2 concentration/ environmental CO2 concentration (Ci/Ca), variable fluorescence (Fv), variable fluorescence/ initial fluorescence (Fv/Fo) and PS Ⅱ primary light energy conversion efficiency (Fv/Fm) decreased gradually with the prolonged waterlogging, and reached their bottom on day 45. During the terminal recovery stage, the leaf gas exchange and chlorophyll fluorescence parameters of the most clones increased, but their recovery abilities were significantly different. At the end of the experiment, the highest survival rates (100%) were observed in DHY, HS-1, HS-2, I-72, I-69, I-63 and NL-895, and the lowest (zero) occurred in XYY. Survival rates of the other clones ranged from 33.33% to 83.33%. Both results of cluster analysis and membership function analysis showed that HS-1, I-69, DHY, NL-895 and HS-2 had the strongest waterlogging tolerance, XYY and HBY were the worst, and the other clones were moderate. These results would provide guidance not only for the selection of cultivated varieties in Jianghan Plain, but also for the selection of hybrid parents for waterlogging resistance breeding.


Azizi S, Tabari M, Striker GG (2017). Growth, physiology, and leaf ion concentration responses to long-term flooding with fresh or saline water of Populus euphratica. South African Journal of Botany 108:229-236. https://doi.org/10.1016/j.sajb.2016.11.004

Bailey-Serres J, Voesenek L (2008). Flooding stress: acclimations and genetic diversity. Annual Review of Plant Biology 59:313-339. https://doi.org/10.1146/annurev.arplant.59.032607.092752

Bejaoui Z, Albouchi A, Lamhamedi MS, Abassi M, Aouni MHE (2012). Adaptation and morpho-physiology of three Populus deltoides Marsh. × P. nigra L. clones after preconditioning to prolonged waterlogging. Agroforestry Systems 86(3):433-442. https://doi.org/10.1007/s10457-012-9487-7

Bilger W, Schreiber U, Bock M (1995). Determination of the quantum efficiency of photosystem II and of non-photochemical quenching of chlorophyll fluorescence in the field. Oecologia 102(4):425-432. http://doi.org/10.1007/BF00341354

Blanke MM, Cooke DT (2004). Effects of flooding and drought on stomatal activity, transpiration, photosynthesis, water potential and water channel activity in strawberry stolons and leaves. Plant Growth Regulation 42: 153-160. https://doi.org/10.1023/B:GROW.0000017489.21970.d4

Cao FL, Conner WH (1999). Selection of flood-tolerant populus deltoides clones for reforestation projects in China. Forest Ecology and Management 117(1-3):211-220. https://doi.org/10.1016/S0378-1127(98)00465-4

Chen LY, Du KB, Jiang FX, Peng YJ, Tu BK, Wamg X (2015). Influences of waterlogging stress on cell structure of primary roots of two poplar species. Scientia Silvae Sinicae 3:163-169. http://en.cnki.com.cn/Article_en/CJFDTOTAL-LYKE201503021.htm

Chen H, Qualls RG, Blank RR (2005). Effect of soil flooding on photosynthesis, carbohydrate partitioning and nutrient uptake in the invasive exotic Lepidium latifolium. Aquatic Botany 82(4):250-268. https://doi.org/10.1016/j.aquabot.2005.02.013

Chen H, Zamorano MF, Ivanoff D (2010). Effect of flooding depth on growth, biomass, photosynthesis, and chlorophyll fluorescence of Typha domingensis. Wetlands 30(5):957-965. https://doi.org/10.1007/s13157-010-0094-y

Correia O, Barradas MCD (2000). Ecophysiological differences between male and female plants of Pistacia lentiscus L. Plant Ecology 149(2):131-142. https://doi.org/10.1023/A:1026588326204

Du KB, Shen BX, Xu L, Tu BK (2008). Estimation of genetic variances in flood tolerance of poplar and selection of resistant F1 generations. Agroforestry Systems 74(3):243-257. https://doi.org/10.1007/s10457-008-9112-y

Du KB, Xu L, Tu BK, Shen BX (2010). Influences of soil flooding on ultrastructure and photosynthetic capacity of leaves of one-year old seedlings of two poplar clones. Scientia Silvae Sinicae 46(6):58-64. http://en.cnki.com.cn/Article_en/CJFDTOTAL-LYKE201006010.htm

Gong JR, Zhang XS, Huang YM, Zhang CL (2007). The effects of flooding on several hybrid poplar clones in Northern China. Agroforestry Systems 69(1):77-88. https://doi.org/10.1007/s10457-006-9019-4

He YY, Wang CY, Yuan ZX, Li XX, Yang WH, Song H, Li CX (2018). Photosynthetic characteristics of Taxodium ascendens and Taxodium distichum under different submergence in the hydro-fluctuation belt of the Three Gorges Reservoir. Acta Ecologica Sinica 8:2722-2731. http://en.cnki.com.cn/Article_en/CJFDTOTAL-STXB201808011.htm

Jackson MB, Saker LR, Crisp CM, Else MA, Janowiak F (2003). Ionic and pH signalling from roots to shoots of flooded tomato plants in relation to stomatal closure. Plant Soil 253:103-113. https://doi.org/10.1023/A:1024588532535

Jiang XM, Hu JY, Qi WH, Chen GD, Xu X (2009). Different physiological responses of male and female Ginkgo biloba (Ginkgoaceae) seedlings to salt stress. Acta Botanica Yunnanica 31(5):447-453. https://doi.org/10.3724/SP.J.1143.2009.09049

Kozlowski TT, Pallardy SG (1984). Effect of flooding on water, carbohydrate, and mineral relations. Flooding and Plant Growth 165-193. http://dx.doi.org/10.1016/B978-0-12-424120-6.50010-9

Kreuzwieser J, Rennenberg H (2014). Molecular and physiological responses of trees to waterlogging stress. Plant Cell Environ 37:2245-2259. https://doi.org/10.1111/pce.12310

Liu Z, Dickmann DI (1992). Responses of two hybrid poplar clones to flooding, drought, and nitrogen availability: I. Morphology and growth. Canadian Journal of Botany 70:2265-2270. https://doi.org/10.1139/b92-281

Liu JL, Wu ZN, Yu YS, Su XH (2014). The waterlogging resistance of ‘Jianghuai’ poplar cultivars. Journal of Forestry Engineering 28(6):25-29. http://dx.doi.org/10.13360/j.issn.1000-8101.2014.06.006

Liu YL, Wang QC, Yang YB, Sun XX (2011). Effects of water stress on growth and photosynthetic characteristics of red pine seedlings. Journal of Northeast Forestry University 39(4):33-36. http://dx.doi.org/10.13759/j.cnki.dlxb.2011.04.038

Loreti E, van Veen H, Perata P (2016). Plant responses to flooding stress. Current Opinion in Plant Biology 33:64-71. http://dx.doi.org/10.1016/j.pbi.2016.06.005

Mielke MS, Almeida de AAF, Gomes FP, Mangabeira PAO, Silva DDC (2005). Effects of soil flooding on leaf gas exchange and growth of two neotropical pioneer tree species. New Forest 29:161-168. http://dx.doi.org/10.1007/s11056-005-0247-7

Pedersen O, Nakayama Y, Yasue H, Kurokawa Y (2020). Lateral roots, in addition to adventitious roots, form a barrier to radial oxygen loss in Zea nicaraguensis and a chromosome segment introgression line in maize. New Phytologist 229(1):5-7. http://doi.org/10.1111/nph.16452

Peng YJ, Zhou ZX, Zhang Z, Yu XL, Zhang XY, Du KB (2018). Molecular and physiological responses in roots of two full-sib poplars uncover mechanisms that contribute to differences in partial submergence tolerance. Scientific Reports 8:12829. http://dx.doi.org/10.1038/s41598-018-30821-y

Peng YJ, Zhou ZX, Tong RG, Hu XY, Du KB (2017). Anatomy and ultrastructure adaptations to soil flooding of two full-sib poplar clones differing in flood-tolerance. Flora 233:90-98. http://dx.doi.org/10.1016/j.flora.2017.05.014

Rodriguez ME, Lauff D, Cortizo S, Luquez VMC (2020). Variability in flooding tolerance, growth and leaf traits in a Populus deltoides intraspecific progeny. Tree Physiology 40(1):19-29. https://doi.org/10.1093/treephys/tpz128

Rood SB, Nielsen, JL, Shenton L, Gill KM, Letts MG (2010). Effects of flooding on leaf development, transpiration, and photosynthesis in narrowleaf cottonwood, a willow-like poplar. Photosynthesis Research 104:31-39. https://doi.org/10.1007/s11120-009-9511-6

Sena Gomes AR, Kozlowski TT (1980). Growth responses and adaptations of Fraxinus pennsylvanica seedlings to flooding. Plant Physiology 66(2):267-271. https://doi.org/10.1104/pp.66.2.267

Smethurst CF, Garnett T, Shabala S (2005). Nutritional and chlorophyll fluorescence responses of lucerne (Medicago sativa) to waterlogging and subsequent recovery. Plant Soil 270:31-45. http://dx.doi.org/doi:10.1007/s11104-004-1082-x

Štícha V, Macků J, Nuhlíček O (2016). Effect of permanent waterlogging on the growth of poplar clones MAX 4, MAX 5 (J-104, J-105) (Populus maximowiczii A. Henry × P. nigra Linnaeus) and evaluation of wood moisture content in different stem parts - short communication. Journal of Forest Science 62:186-190. https://doi.org/10.17221/90/2015-JFS

Striker GG, Colmer TD (2017). Flooding tolerance of forage legumes. Journal of Experimental Botany 68(1851):1851. http://dx.doi.org/10.1093/jxb/erw239

Sun H, Wu ZN, Liu JL, Miao TT, Cao ZH (2020). Effects of water stress on growth and physiological properties of four poplar varieties. Journal of West China Forestry Science 49(2):62-67. https://doi.org/10.16473/j.cnki.xblykx1972.2020.02.010

Voesenek LACJ, Bailey-Serres J (2013). Flooding tolerance: O2 sensing and survival strategies. Current Opinion in Plant Biology 16:647-653. http://dx.doi.org/10.1016/j.pbi.2013.06.008

Yan C, Song S, Wang W, Wang C, Li H, Wang F, Li S, Sun X (2020). Screening diverse soybean genotypes for drought tolerance by membership function value based on multiple traits and drought tolerant coefficient of yield. BMC Plant Biology 20:321. https://doi.org/10.1186/s12870-020-02519-9

Yan Z, Zhang JT, Zhao PH, Yang SH, Ma YT, Zhu D (2019). Effects of continuous waterlogging stress on growth, physiology and biochemistry of Populus deltoides seedlings. Journal of Central South University of Forestry & Technology 39(12):16-23. https://doi.org/10.14067/j.cnki.1673-923x.2019.12.003

Yang P, Xu X (2012). Effects of waterlogging stress on the growth and physiological characteristics of male and female Populus cathayana seedlings. Chinese Journal of Plant Ecology 36(1):81-87. http://en.cnki.com.cn/Article_en/CJFDTOTAL-ZWSB201201012.htm

Zhao XJ, Cheng F, Zhang K, Huang KD, Ni Y, Meng X, Tang LZ (2019). Root morphology of different poplar clone stecklings under waterlogging and flooding treatment. Journal of Nanjing Forestry University 43(5):1-8. http://en.cnki.com.cn/Article_en/CJFDTotal-NJLY201905001.htm

Zhou ZC, Li G, Sun XM, Xu F, Chen ZX (2019). Physiological responses and tolerance evaluation of five poplar varieties to waterlogging. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47(3):658-667. https://doi.org/10.15835/nbha47311440




How to Cite

GENG, R., ZHANG, X., FAN, X., HU, Q., NI, T., & DU, K. (2021). Waterlogging tolerance evaluation of fifteen poplar clones cultivated in the Jianghan Plain of China. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(3), 12421. https://doi.org/10.15835/nbha49312421



Research Articles
DOI: 10.15835/nbha49312421