Light-emitting diode spectra modify nutritional status, physiological response, and secondary metabolites in Ficus hirta and Alpinia oxyphylla

Changwei ZHOU; Chongfei SHANG; Feiyu CHEN; Junzhou BAO; Lifei YU; Peng GUO

doi:10.15835/nbha49212314

Authors

Changwei ZHOU College of Life Science, Guizhou University, Guiyang 550025 (CN)
Chongfei SHANG College of Life Science, Guizhou University, Guiyang 550025 (CN)
Feiyu CHEN College of Life Science, Guizhou University, Guiyang 550025 (CN)
Junzhou BAO College of Life Science, Guizhou University, Guiyang 550025 (CN)
Lifei YU College of Life Science, Guizhou University, Guiyang 550025 (CN)
Peng GUO Environment and Resources College, Dalian Nationalities University, Dalian 116600 (CN)

DOI:

https://doi.org/10.15835/nbha49212314

Keywords:

chlorophyll, flavonoid, light-emitting diode, medicinal plants, saponin, non-structural carbohydrate

Abstract

Lighting spectrum is one of the key factors that determine biomass production and secondary-metabolism accumulation in medicinal plants under artificial cultivation conditions. Ficus hirta and Alpinia oxyphylla seedlings were cultured with blue (10% red, 10% green, 70% blue), green (20% red, 10% green, 30% blue), and red-enriched (30% red, 10% green, 20% blue) lights in a wide bandwidth of 400-700 nm. F. hirta seedlings had lower diameter, fine root length, leaf area, biomass, shoot nutrient (N) and phosphorus concentrations in the blue-light spectrum compared to the red- and green-light spectra. In contrast, A. oxyphylla seedlings showed significantly higher concentrations of foliar flavonoids and saponins in red-light spectrum with rare responses in N, chlorophyll, soluble sugars, and starch concentrations. F. hirta is easily and negatively impacted by blue-light spectrum but A. oxyphylla is suitably used to produce flavonoid and saponins in red-light spectrum across a wide bandwidth.

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References

Ballare CL (2014). Light regulatoin of plant defense. Annual Review of Plant Biology 65:335-363. https://doi.org/10.1146/annurev-arplant-050213-040145

Bhardwaj P, Jain CK, Mathur A (2019). Comparative analysis of saponins, flavonoids, phenolics and antioxidant activities of field acclimatized and in vitro propagated Bacopa monnieri (L.) Pennell from different locations in India. Indian Journal of Experimental Biology 57(4):259-268. https://doi.org/handle/123456789/46928

Claypool NB, Lieth JH (2020). Physiological responses of pepper seedlings to various ratios of blue, green, and red light using LED lamps. Scientia Horticulturae 268:10. https://doi.org/10.1016/j.scienta.2020.109371

Duan J, Xu CY, Jacobs DF, Ma LY, Wei HX, Jiang LN, Ren J (2013). Exponential nutrient loading shortens the cultural period of Larix olgensis seedlings. Scandinavian Journal of Forest Research 28(5):409-418. https://doi.org/10.1080/02827581.2013.778328

Elmlinger MW, Mohr H (1992). Glutamine synthetase in Scots pine seedlings and its control by blue light and light absorbed by phytochrome. Planta 188(3):396-402. https://doi.org/10.1007/BF00192807

Folta KM, Maruhnich SA (2007). Green light: a signal to slow down or stop. Journal of Experimental Botany 58(12):3099-3111. https://doi.org/10.1093/jxb/erm130

Golawska S, Krzyzanowski R, Lukasik I (2010). Relationship between aphid infestation and chlorophyll content in Fabaceae species. Acta Biologica Cracoviensia Series Botanica 52(2):76-80. https://doi.org/10.2478/v10182-010-0026-4

Holopainen JK, Kivimaenpaa M, Julkunen-Tiitto R (2018). New light for phytochemicals. Trends in Biotechnology 36(1):7-10. https://doi.org/10.1016/j.tibtech.2017.08.009

Kang WH, Park JS, Park KS, Son JE (2016). Leaf photosynthetic rate, growth, and morphology of lettuce under different fractions of red, blue, and green light from light-emitting diodes (LEDs). Horticulture Environment and Biotechnology 57(6):573-579. https://doi.org/10.1007/s13580-016-0093-x

Kinoshita T, Shimazaki K (1997). Involvement of calyculin A- and okadaic acid-sensitive protein phosphatase in the blue light response of stomatal guard cells. Plant and Cell Physiology 38(11):1281-1285. https://doi.org/10.1093/oxfordjournals.pcp.a029117

Landi M, Zivcak M, Sytar O, Brestic M, Allakhverdiev SI (2020). Plasticity of photosynthetic processes and the accumulation of secondary metabolites in plants in response to monochromatic light environments: A review. Biochimica Et Biophysica Acta-Bioenergetics 1861(2):24. https://doi.org/10.1016/j.bbabio.2019.148131

Li XW, Chen QX, Lei HQ, Wang JW, Yang S, Wei HX (2018). Nutrient uptake and utilization by fragrant rosewood (Dalbergia odorifera) seedlings cultured with oligosaccharide addition under different lighting spectra. Forests 9(1):15. https://doi.org/10.3390/f9010029

Li XW, Gao Y, Wei HX, Xia HT, Chen QX (2017). Growth, biomass accumulation and foliar nutrient status in fragrant rosewood (Dalbergia odorifera TC Chen) seedlings cultured with conventional and exponential fertilizations under different photoperiod regimes. Soil Science and Plant Nutrition 63(2):153-162. https://doi.org/10.1080/00380768.2017.1312518

Luo YQ, Zhao SJ, Tang JY, Zhu H, Wei HX, Cui W, Wang MH, Guo P (2020). White-light emitting diodes' spectrum effect on photosynthesis and nutrient use efficiency in Podocarpus macrophyllus seedlings. Journal of Plant Nutrition 9. https://doi.org/10.1080/01904167.2020.1798999

Ma XH, Qian RJ, Zhang XL, Hu QD, Liu HJ, Zheng J (2019). Contrasting growth, physiological and gene expression responses of Clematis crassifolia and Clematis cadmia to different irradiance conditions. Scientific Reports 9:12. https://doi.org/10.1038/s41598-019-54428-z

Manivannan A, Soundararajan P, Halimah N, Ko CH, Jeong BR (2015). Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Relunannia glutinosa cultured in vitro. Horticulture Environment and Biotechnology 56(1):105-113. https://doi.org/10.1007/s13580-015-0114-1

Nam TG, Kim DO, Eom SH (2018). Effects of light sources on major flavonoids and antioxidant activity in common buckwheat sprouts. Food Science and Biotechnology 27(1):169-176. https://doi.org/10.1007/s10068-017-0204-1

Ni J, Dong LX, Jiang ZF, Yang XL, Sun ZH, Li JX, ... Xu MJ (2018). Salicylic acid-induced flavonoid accumulation in Ginkgo biloba leaves is dependent on red and far-red light. Industrial Crops and Products 118:102-110. https://doi.org/10.1016/j.indcrop.2018.03.044

Nishio JN (2001). Why are higher plants green? Evolution of the higher plant photosynthetic pigment complement. Plant, Cell & Environment 23:539-548. https://doi.org/10.1046/j.1365-3040.2000.00563.x

Niu Q, Gao YM, Liu PH (2020). Optimization of microwave-assisted extraction, antioxidant capacity, and characterization of total flavonoids from the leaves of Alpinia oxyphylla Miq. Preparative Biochemistry & Biotechnology 50(1):82-90. https://doi.org/10.1080/10826068.2019.1663535

Pedone-Bonfim MVL, Lins MA, Coelho IR, Santana AS, Silva FSB, Maia LC (2013). Mycorrhizal technology and phosphorus in the production of primary and secondary metabolites in cebil (Anadenanthera colubrina (Vell.) Brenan) seedlings. Journal of the Science of Food and Agriculture 93(6):1479-1484. https://doi.org/10.1002/jsfa.5919

Pennisi G, Pistillo A, Orsini F, Cellini A, Spinelli F, Nicola S, ... Marcelis LFM (2020). Optimal light intensity for sustainable water and energy use in indoor cultivation of lettuce and basil under red and blue LEDs. Scientia Horticulturae 272:10. https://doi.org/10.1016/j.scienta.2020.109508

Qi MM, Hua XY, Peng XY, Yan XF, Lin JX (2018). Comparison of chemical composition in the buds of Aralia elata from different geographical origins of China. Royal Society Open Science 5(8):10. https://doi.org/10.1098/rsos.180676

Rehman M, Ullah S, Bao YN, Wang B, Peng DX, Liu LJ (2017). Light-emitting diodes: whether an efficient source of light for indoor plants? Environmental Science and Pollution Research 24(32):24743-24752. https://doi.org/10.1007/s11356-017-0333-3

Sabzalian MR, Heydarizadeh P, Zahedi M, Boroomand A, Agharokh M, Sahba MR, Schoefs B (2014). High performance of vegetables, flowers, and medicinal plants in a red-blue LED incubator for indoor plant production. Agronomy for Sustainable Development 34(4):879-886. https://doi.org/10.1007/s13593-014-0209-6

Stankovic J, Godevac D, Tesevic V, Dajic-Stevanovic Z, Ciric A, Sokovic M, Novakovic M (2019). Antibacterial and antibiofilm activity of flavonoid and saponin derivatives from Atriplex tatarica against Pseudomonas aeruginosa. Journal of Natural Products 82(6):1487-1495. https://doi.org/10.1021/acs.jnatprod.8b00970

Taulavuori K, Hyoky V, Oksanen J, Taulavuori E, Julkunen-Tiitto R (2016). Species-specific differences in synthesis of flavonoids and phenolic acids under increasing periods of enhanced blue light. Environmental and Experimental Botany 121:145-150. https://doi.org/10.1016/j.envexpbot.2015.04.002

Terashima I, Fujita T, Inoue T, Chow WS, Oguchi R (2009). Green light drives leaf photosynthesis more efficiently than red light in strong white light: revisiting the enigmatic question of why leaves are green. Plant and Cell Physiology 50:684-697. https://doi.org/10.1093/pcp/pcp034

Wan FF, Ross-Davis AL, Davis AS, Song XH, Chang XC, Zhang J, Liu Y (2020). Nutrient retranslocation in Larix principis-rupprechtii Mayr relative to fertilization and irrigation. New Forests https://doi.org/10.1007/s11056-020-09783-5

Wan FF, Ross-Davis AL, Shi WH, Weston C, Song XH, Chang XC, ... Teng F (2019). Subirrigation effects on larch seedling growth, root morphology, and media chemistry. Forests 10(1):14. https://doi.org/10.3390/f10010038

Wang R, Wang Y, Su Y, Tan JH, Luo XT, Li JY, He Q (2020). Spectral effect on growth, dry mass, physiology and nutrition in Bletilla striata seedlings: Individual changes and collaborated response. International Journal of Agriculture and Biology 24(1):125-132. https://doi.org/10.17957/ijab/15.1416

Watcharatanon K, Ingkaninan K, Putalun W (2019). Improved triterpenoid saponin glycosides accumulation in in vitro culture of Bacopa monnieri (L.) Wettst with precursor feeding and LED light exposure. Industrial Crops and Products 134:303-308. https://doi.org/10.1016/j.indcrop.2019.04.011

Wei HX, Chen GS, Chen X, Zhao HT (2020a). Growth and nutrient uptake in Aralia elata seedlings exposed to exponential fertilization under different illumination spectra. International Journal of Agriculture and Biology 23(3):644-652. https://doi.org/10.17957/ijab/15.1336

Wei HX, Chen X, Chen GS, Zhao HT (2019). Foliar nutrient and carbohydrate in Aralia elata can be modified by understory light quality in forests with different structures at Northeast China. Annals of Forest Research 62(2):125-137. https://doi.org/10.15287/afr.2019.1395

Wei HX, Hauer RJ, Chen GS, Chen X, He XY (2020b). Growth, nutrient assimilation, and carbohydrate metabolism in Korean pine (Pinus koraiensis) seedlings in response to light spectra. Forests 11(1):18. https://doi.org/10.3390/f11010044

Wei HX, Ren J, Zhou JH (2013). Effect of exponential fertilization on growth and nutritional status in Buddhist pine (Podocarpus macrophyllus Thunb. D. Don) seedlings cultured in natural and prolonged photoperiods. Soil Science and Plant Nutrition 59(6):933-941. https://doi.org/10.1080/00380768.2013.864957

Wei HX, Zhao HT, Chen X (2019). Foliar N:P stoichiometry in Aralia elata distributed on different slope degrees. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47(3):887-895. https://doi.org/10.15835/nbha47311390

World Health Organization (2013). WHO Traditional Medicine Strategy: 2014-2013. Geneva, Switzerland: World Health Organization.

Xu L, Zhang X, Zhang DH, Wei HX, Guo J (2019). Using morphological attributes for the fast assessment of nutritional responses of Buddhist pine (Podocarpus macrophyllus Thunb. D. Don) seedlings to exponential fertilization. Plos One 14(12):14. https://doi.org/10.1371/journal.pone.0225708

Ya J, Zhang XQ, Wang Y, Zhang QW, Chen JX, Ye WC (2010). Two new phenolic compounds from the roots of Ficus hirta. Natural Product Research 24(7):621-625. https://doi.org/10.1080/14786410902847377

Yan ZN, He DX, Niu GH, Zhou Q, Qu YH (2020). Growth, nutritional quality, and energy use efficiency in two lettuce cultivars as influenced by white plus red versus red plus blue LEDs. International Journal of Agricultural and Biological Engineering 13(2):33-40. https://doi.org/10.25165/j.ijabe.20201302.5135

Yu WW, Liu Y, Song LL, Jacobs DF, Du XH, Ying YQ, ... Wu JS (2017). Effect of differential light quality on morphology, photosynthesis, and antioxidant enzyme activity in Camptotheca acuminata seedlings. Journal of Plant Growth Regulation 36(1):148-160. https://doi.org/10.1007/s00344-016-9625-y

Zhang T, Shi YY, Piao FZ, Sun ZQ (2018). Effects of different LED sources on the growth and nitrogen metabolism of lettuce. Plant Cell Tissue and Organ Culture 134(2):231-240. https://doi.org/10.1007/s11240-018-1415-8

Zhao J, Chen X, Wei HX, Lv J, Chen C, Liu XY, ... Jia LM (2019). Nutrient uptake and utilization in Prince Rupprecht's larch (Larix principis-rupprechtii Mayr.) seedlings exposed to a combination of light-emitting diode spectra and exponential fertilization. Soil Science and Plant Nutrition 65(4):358-368. https://doi.org/10.1080/00380768.2019.1631715

Zhao J, Thi LT, Park YG, Jeong BR (2020) Light quality affects growth and physiology of carpesium triste Maxim. cultured in vitro. Agriculture-Basel 10(7):19. https://doi.org/10.3390/agriculture10070258

Zu YQ, Mei XY, Li B, Li T, Li Q, Qin L, Yang ZQ (2020) Effects of calcium application on the yields of flavonoids and saponins in Panax notoginseng under cadmium stress. International Journal of Environmental Analytical Chemistry 12. https://doi.org/10.1080/03067319.2020.1781835