Molecular weight and concentration of chitosan affect plant development and phenolic substance pattern in arugula

Arda ACEMİ; Ece GÜN POLAT; Merve  ÇAKIR; Elif DEMİRYÜREK; Bahar YAVUZ; Fazıl ÖZEN

doi:10.15835/nbha49212296

Authors

Arda ACEMİ Kocaeli University, Faculty of Arts and Sciences, Department of Biology, 41001 İzmit, Kocaeli (TR)
Ece GÜN POLAT Kocaeli University, Faculty of Arts and Sciences, Department of Biology, 41001 İzmit, Kocaeli (TR)
Merve ÇAKIR Kocaeli University, Faculty of Arts and Sciences, Department of Biology, 41001 İzmit, Kocaeli (TR)
Elif DEMİRYÜREK Kocaeli University, Faculty of Arts and Sciences, Department of Biology, 41001 İzmit, Kocaeli (TR)
Bahar YAVUZ Kocaeli University, Faculty of Arts and Sciences, Department of Biology, 41001 İzmit, Kocaeli (TR)
Fazıl ÖZEN Kocaeli University, Faculty of Arts and Sciences, Department of Biology, 41001 İzmit, Kocaeli (TR)

DOI:

https://doi.org/10.15835/nbha49212296

Keywords:

biopolymer, chitin, Eruca sativa, leaf development, phenolic pattern, rhizogenesis

Abstract

The present research reports the role of chitosan’s molecular weight (1, 10, and 100 kDa) on the differentiation of its effects on arugula (Eruca vesicaria ssp. sativa) cultivation in a controlled environment. The leaves' phenolic substance pattern from the plants treated with the chitosan variant that gave the best developmental results was analyzed through a reversed-phase HPLC. The leaf production was enhanced after 10 kDa chitosan treatment at 5 mg L^-1, while the leaf area expansion was significantly improved after 1 and 100 kDa chitosan at 20 mg L^-1 and 10 kDa chitosan at 5 mg L^-1. The plant's rhizogenic development was restricted after all chitosan treatments regardless of their molecular weight and concentration. The contents of chlorophyll b and carotenoids increased after the treatments; however, chlorophyll a content was not significantly affected by the treatments and remained unchanged. The chromatographic analysis showed that 10 kDa chitosan treatment at 5 mg L^-1 increased gallic acid, rutin, and p-coumaric acid contents and made significant changes in the individual phenolic substance pattern. The current study indicated that direct application of chitosan to soil restricts root production in arugula but enhances foliar growth, which is beneficial to producers. On the other hand, constant- or over-treatment with chitosan could inhibit root growth and further lead to developmental deficiencies sourced by nutrient uptake disorders. The use of chitosan as an organic and natural biostimulant in controlled-environment agriculture could be a better option than synthetic growth stimulants.

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References

Acemi A, Bayrak B, Çakir M, Demiryürek E, Gün E, El Gueddari NE, Özen F (2018). Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants. In Vitro Cellular and Developmental Biology - Plant 54:537-544. https://doi.org/10.1007/s11627-018-9915-0

Acemi A (2020a). Chitosan versus plant growth regulators: a comparative analysis of their effects on in vitro development of Serapias vomeracea (Burm.f.) Briq. Plant Cell, Tissue and Organ Culture 141:327-338. https://doi.org/10.1007/s11240-020-01789-3

Acemi A (2020b). Polymerization degree of chitosan affects structural and compositional changes in the cell walls, membrane lipids, and proteins in the leaves of Ipomoea purpurea: An FT-IR spectroscopy study. International Journal of Biological Macromolecules 162:715-722. https://doi.org/10.1016/j.ijbiomac.2020.06.171

Ahmad B, Khan MMA, Jaleel H, Sadiq Y, Shabbir A, Uddin M (2017). Exogenously sourced γ-irradiated chitosan-mediated regulation of growth, physiology, quality attributes and yield in Mentha piperita L. Turkish Journal of Biology 41(2):388-401. https://doi.org/10.3906/biy-1608-64

Al-Mohammad MHS, Al-Taey DKA (2019). Effect of tyrosine and sulfur on growth, yield and antioxidant compounds in arugula leaves and seeds. Research on Crops 20(1):116-120. https://doi.org/10.31830/2348-7542.2019.016

Barber MS, Bertram RE, Ride JP (1989). Chitin oligosaccharides elicit lignification in wounded wheat leaves. Physiological and Molecular Plant Pathology 34:3-12. https://doi.org/10.1016/0885-5765(89)90012-X

Benke K, Tomkins B (2018). Future food-production systems: vertical farming and controlled environment agriculture. Sustainability: Science, Practice and Policy 13(1):13-26. https://doi.org/10.1080/15487733.2017.1394054

Boonlertnirun S, Suvannasara R, Promsomboon P, Boonlertnirun K (2011). Application of chitosan for reducing chemical fertilizer uses in waxy corn growing. Thai Journal of Agricultural Science 44(5):22-28.

Chamnanmanoontham N, Pongprayoon W, Pichayangkura R, Roytrakul S, Chadchawan S (2015). Chitosan enhances rice seedling growth via gene expression network between nucleus and chloroplast. Plant Growth Regulation 75:101-114. https://doi.org/10.1007/s10725-014-9935-7

Dar TA, Uddin M, Khan MMA, Ali A, Mir SR, Varshney L (2015). Effect of Co-60 gamma irradiated chitosan and phosphorus fertilizer on growth, yield and trigonelline content of Trigonella foenum-graecum L. Journal of Radiation Research and Applied Sciences 8(3):446-458. https://doi.org/10.1016/j.jrras.2015.03.008

Darvill A, Augur C, Bergmann C, Carlson RW, Cheong J-J, Eberhard S, … Albersheim P (1992). Oligosaccharins-oligosaccharides that regulate growth, development and defence responses in plants. Glycobiology 2(3):181-198. https://doi.org/10.1093/glycob/2.3.181

Jaleel H, Khan MMA, Ahmad B, Shabbir A, Sadiq Y, Uddin M, Varshney L (2017). Essential oil and citral production in field-grown lemongrass in response to gamma-irradiated chitosan. Journal of Herbs, Spices and Medicinal Plants 23(4):378-392. https://doi.org/10.1080/10496475.2017.1349702

Johnson RE, Kong Y, Zheng Y (2020). Elongation growth mediated by blue light varies with light intensities and plant species: A comparison with red light in arugula and mustard seedlings. Environmental and Experimental Botany 169:103898. https://doi.org/10.1016/j.envexpbot.2019.103898

Khan WM, Prithiviraj B, Smiyh DL (2002). Effect of foliar application of chitin and chitosan oligosaccharides on photosynthesis of maize and soybean. Photosynthetica 40:621-624. https://doi.org/10.1023/A:1024320606812

Kıran Acemi R, Acemi A (2019). Polymerization degree-dependent changes in the effects of in vitro chitosan treatments on photosynthetic pigment, protein, and dry matter contents of Ipomoea purpurea. EuroBiotech Journal 3(4):197-202. https://doi.org/10.2478/ebtj-2019-0024

Kıran Acemi R, Acemi A, Çakır M, Gün Polat E, Özen F (2020). Preliminary screening the antioxidant potential of in vitro-propagated Amsonia orientalis: An example to sustainable use of rare medicinal plants in pharmaceutical studies. Sustainable Chemistry and Pharmacy 17:100302. https://doi.org/10.1016/j.scp.2020.100302

Ladaru G-R, Ilie DM, Diaconeasa MC, Petre IL, Marin F, Lazar V (2020). Influencing factors of a sustainable vegetable choice. The Romanian consumers’ case. Sustainability 12:9991. https://doi.org/10.3390/su12239991

Lichtenthaler H (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology 148:350-382. https://doi.org/10.1016/0076-6879(87)48036-1

Metsalu T, Vilo J (2015). ClustVis: a web tool for visualizing clustering of multivariate data using principal component analysis and heatmap. Nucleic Acids Research 43(W1):W566-W570. https://doi.org/10.1093/nar/gkv468

Mondal M, Puteh AB, Dafader NC (2016). Foliar application of chitosan improved morpho-physiological attributes and yield in summer tomato (Solanum lycopersicum). Pakistan Journal of Agricultural Sciences 53(2):339-344. https://doi.org/10.21162/PAKJAS/16.2011

Mondal MMA, Malek MA, Puteh AB, Ismail MR (2013). Foliar application of chitosan on growth and yield attributes of mungbean (Vigna radiata (L.) Wilczek). Bangladesh Journal of Botany 42(1):179-183. https://doi.org/10.3329/bjb.v42i1.15910

Mondal MMA, Rana IK, Dafader NC, Haque ME (2011). Effect of foliar application of chitosan on growth and yield in Indian spinach. Journal of Agroforestry and Environment 5(1):99-102.

Monirul IM, Humayun KM, Mamun ANK, Monirul I, Pronabananda D (2018). Studies on yield and yield attributes in tomato and chilli using foliar application of oligo-chitosan. GSC Biological and Pharmaceutical Sciences 3(3):20-28. https://doi.org/10.30574/gscbps.2018.3.3.0038

Morales M, Janick J (2002). Arugula: A promising specialty leaf vegetable. In: Janick J, Whipkey A (Eds). Trends in New Crops and New Uses. ASHS Press, Alexandria, VA, USA, pp 418-423.

Muley AB, Shingote PR, Patil AP, Dalvi SG, Suprasanna P (2019). Gamma radiation degradation of chitosan for application in growth promotion and induction of stress tolerance in potato (Solanum tuberosum L.). Carbohydrate Polymers 210:289-301. https://doi.org/10.1016/j.carbpol.2019.01.056

Muxika A, Etxabide A, Uranga J, Guerrero P, de la Caba K (2017). Chitosan as a bioactive polymer: Processing, properties and applications. International Journal of Biological Macromolecules 105(2):1358-1368. https://doi.org/10.1016/j.ijbiomac.2017.07.087

Nge KL, New N, Chandrkrachang S, Stevens WF (2006). Chitosan as a growth stimulator in orchid tissue culture. Plant Science 170(6):1185-1190. https://doi.org/10.1016/j.plantsci.2006.02.006

Pandey SK, Singh H (2011). A simple, cost-effective method for leaf area estimation. Journal of Botany 2011:658240. https://doi.org/10.1155/2011/658240

Rahman M, Mukta JA, Sabir AA, Gupta DR, Mohi-Ud-Din M, Hasanuzzaman M, … Islam T (2018). Chitosan biopolymer promotes yield and stimulates accumulation of antioxidants in strawberry fruit. PLoS ONE 13(9):e0203769. https://doi.org/10.1371/journal.pone.0203769

Romanazzi G, Feliziani E, Baños SB, Sivakumar D (2015). Shelf life extension of fresh fruit and vegetables by chitosan treatment. Critical Reviews in Food Science and Nutrition 57(3):579-601. https://doi.org/10.1080/10408398.2014.900474

Rugeles-Reyes SM, Cecílio Filho AB, López Aguilar MA, Silva PHS (2019). Foliar application of zinc in the agronomic biofortification of arugula. Food Science and Technology 39(4):1011-1017. https://doi.org/10.1590/fst.12318

Sahariah P, Másson M (2017). Antimicrobial chitosan and chitosan derivatives: A review of the structure–activity relationship. Biomacromolecules 18(11):3846-3868. https://doi.org/10.1021/acs.biomac.7b01058

Santiago FEM, Silva MLS, Cardoso AAS, Duan Y, Guilherme LRG, Liu J, Li L (2020). Biochemical basis of differential selenium tolerance in arugula (Eruca sativa Mill.) and lettuce (Lactuca sativa L.). Plant Physiology and Biochemistry 157:328-338. https://doi.org/10.1016/j.plaphy.2020.11.001

Srisornkompon P, Pichyangkura R, Chadchawan S (2014). Chitosan increased phenolic compound contents in tea (Camellia sinensis) leaves by pre- and post-treatments. Journal of Chitin and Chitosan Science 2:1-6. https://doi.org/10.1166/jcc.2014.1056

Theerakarunwong CD, Phothi R (2016). Physiological and photosynthesis enhancement of Thai rice (Oryza sativa L.) cultivars by chitosan. NU. International Journal of Science 13(1):37-49.

Uthairatanakij A, Teixeira da Silva JA, Obsuwan K (2007). Chitosan for improving orchid production and quality. Orchid Science and Biotechnology 1(1):1-5.

Youssef NH (2018). Assessment of aflatoxins produced by certain Aspergilla in heavy metals contaminated soil treated with commercial chitosan. International Journal of Current Research 10(7):71562-71567. https://doi.org/10.24941/ijcr.30642.07.2018

Zeng D, Luo X (2012). Physiological eﬀects of chitosan coating on wheat growth and activities of protective enzyme with drought tolerance. Open Journal of Soil Science 2:282-288. https://doi.org/10.4236/ojss.2012.23034