Effect of digestate from anaerobic digestion on Sorghum bicolor L. production and circular economy

  • Vera POPOVIĆ Institute of Field and Vegetable Crops, Maksim Gorky 30, Novi Sad
  • Nikola RAKASCAN Singidunum University, Danijelova 32, Belgrade
  • Gordana DRAZIC Singidunum University, Danijelova 32, Belgrade
  • Jelena MILOVANOVIC Singidunum University, Danijelova 32, Belgrade
  • Ljubisa ZIVANOVIC University of Belgrade, Faculty of Agriculture, Nemanjina 6, Belgrade
  • Milena ACIMIC REMIKOVIC University of Montenegro, Faculty of Law, July 13, Podgorica
  • Tijana MILANOVIC University of Belgrade Faculty of Organizational Sciences, Jove Ilica 154, Belgrade
  • Jela IKANOVIC University of Belgrade, Faculty of Agriculture, Nemanjina 6, Belgrade
Keywords: agri-energy crop; biogas; bio-fertilization; circular economy; marginal soil


The aim of the research was determination the efficiency of application 50 t ha-1 digestate from the process of anaerobic digestion on the productivity of sorghum (Sorghum bicolor L.) on moderately degraded (calcic gleysol) and fertile (chernozem) agricultural land, in southern Banat, Republic of Serbia. In the field experiment during three years digestate amendment led to an increase in the number of leaves by 28.56% and plant height by 5.34%, which led to an increase in yield by 3.40%. The maximum yield was 2018 (41.74 DM t ha-1) on chernozem. The yield of sorghum was lower on calcic gleysol compared to chernozem by 5.43% and was in positive, medium and very significant dependence on precipitation (0.61) and in positive significant correlation with digestate (0.53) and plant height (0.59). Biogas yield reach 157.05 Nm3 t-1 (9582 Nm3 ha-1) on chernozem with digestate. Digestate had a statistically significant positive effect on all tested characteristics of sorghum as well as biogas yield during all three experimental years. The use of digestate as a by-product in the process of producing biogas based on silage of sorghum, allows the substitution of mineral fertilizers and remediation of damaged soil, which contributes to sustainability from the bio-economic and environmental aspects.


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Agostini A, Battini F, Guintoli J, Zabaglio V, Padella M, Baxter D, … Amaduci S (2015). Environmentally sustainable biogas? The key role of maize co-digestion with energy crops. Energies 8:5234-5265. https://doi.org/10.3390/en8065234

Ameen A, Yang X, Chen F, Tang C, Du F, Fahad S, Hui Xie G (2017). Biomass yield and nutrient uptake of energy sorghum in response to nitrogen fertilizer rate on marginal land in a semi-arid region. Bioenergy Resources 10:363-376. https://doi.org/10.1007/s12155-016-9804-5

Amon T, Amon B, Kryvoruchko V, Machmüller A, Hopfner-Sixt K, Bodiroza V, … Zollitschf W (2007). Methane production through anaerobic digestion of various energy crops grown in sustainable crop rotations. Bioresource Technology 98(17):3204-3212. https://doi.org/10.1016/j.biortech.2006.07.007

Barbosa D, Nabel M, Jablonowski N D (2014). Biogas-digestate as nutrient source for biomass production of Sida hermaphrodita, Zea mays L. and Medicago sativa L. Energy Procedia 59:120-126. https://doi.org/10.1016/j.egypro.2014.10.357

De Meester S, Demeyer J, Velghe F, Peene A, Van Langenhove H, Dewulf J (2012). The environmental sustainability of anaerobic digestion as a biomass valorization technology. Bioresource Technology 121:396-403. https://doi.org/10.1016/j.biortech.2012.06.109

Duque-Acevedo M, Belmonte-Ureña LJ, Yakovleva N, Camacho-Ferre F (2020). Analysis of the circular economic production models and their approach in agriculture and agricultural waste biomass management. Journal of Environmental Research and Public Health 17:9549. https://doi.org/10.3390/ijerph17249549

European Parliament (2009). Directive 2009/28/EC. Promotion of the use of energy from renewable sources and amending and subsequently repealing Directives 2001/77/EC and 2003/30/EC. O. J. European Union 140(16). http://data.europa.eu/eli/dir/2009/28/2015-10-05

Garofalo P, Rinaldi M (2013). Water-use efficiency of irrigated biomass sorghum in a Mediterranean environment. Spanish Journal of Agricultural Research 11:1153-1169.

Gelaye KK, Zehetner F, Loiskandl W, Klik A (2019). Comparison of growth of annual crops used for salinity bioremediation in the semi-arid irrigation area. Plant Soil Environment 65:165-171. https://doi.org/10.17221/499/2018-PSE

Głab L, Sowinski J (2019). Sustainable production of sweet sorghum as a bioenergy crop using biosolids taking into account greenhouse gas emissions. Sustainability 11:3033-3050. https://doi.org/10.3390/su11113033

Habyarimana E, Piccard I, Catellani M, De Franceschi P, Dall’agata M (2019). Towards predictive modeling of sorghum biomass yields using fraction of absorbed photosynthetically active radiation derived from Sentinel-2 Satellite Imagery and Supervised Machine Learning Techniques. Agronomy 9(4):203. https://doi.org/10.3390/agronomy9040203

Hao X, Thomas B, Nelson V, Li X (2016). Agronomic values of anaerobically digested cattle manure and the separated solids for barley forage production. Soil Fertility & Plant Nutrition 80:1572-1584. https://doi.org/10.2136/sssaj2016.05.0132

Hassan MU, Chattha MU, Mahmood A, Sahi ST (2018). Performance of sorghum cultivars for biomass quality and biomethane yield grown in semi-arid area of Pakistan. Environmental Science and Pollution Research 25:12800-12807. https://doi.org/10.1007/s11356-018-1575-4

Ikanović J, Glamoclija DJ, Maletic R, Popović V, Sokolović D, Spasić M, Rakić S (2011). Path analysis of the productive traits in Sorghum species. Genetika 43(2):253-262. https://doi.org/10.2298/GENSR1102253I

Ikanović J, Živanović Lj, Popović V, Kolarić Lj, Dražić G, Janković S, Pavlović S (2018). Possibility of greater use of maize as a bioenergy. Journal of PKB Institute of Agroekonomik 24(1-2):49-59.

Jankovic S, Glamoclija DJ, Prodanovic S (2017). Energy crops - Technology for production and processing. Belgrade, ISBN 987-86-81689-35-6, pp 1-410. (In Serbian).

Kirubakarana V, Sivaramakrishnanb V, Nalinic R, Sekard T, Premalathae M, Subramaniane P (2009). A review on gasification of biomass. Renewable and Sustainable Energy Reviews 13:179-186. https://doi.org/10.1016/j.rser.2007.07.001

Lakic Z, Glamoclija DJ, Popovic V, Pavlovic S, Kondic D (2018). Fodder plants and cereals in the function of soil protection against degradation. Faculty of Agriculture. Banja Luka 269-275 (In Serbian).

Mahmood A, Ullah H, Ijaz M, Javaid M, Shahzad AN, Honermeier B (2013) Evaluation of sorghum hybrids for biomass and biogas production. Australian Journal for Crop Sciences 7(10):1456-1462.

Mahmood A, Abrar H, Shahzad A N, Bernd H (2015). Biomass and biogas yielding potential of sorghum as affected by planting density, sowing time and cultivar. Pakistan Journal of Botany 47:2401-2408.

Makadi M, Tomocsik A, Orosz V (2012). Digestate: A new nutrient source - a review. In: Kumar S (Ed). Biogas. In Tech, pp 295-310. https://doi.org/10.5772/31355

Milanović T, Popović V, Vučković S, Rakaščan N, Popović S, Petković Z (2020). Analysis of soybean production and biogas yield to improve eco-marketing and circular economy. Economics of Agriculture, Belgrade, 67(1):141-156. https://doi.org/10.5937/ekoPolj2001141M

Mekdad A, Rady M (2016). Productivity response to plant density in five Sorghum bicolor varieties in dry environments. Annals of Agricultural & Crop Sciences 1(2):531-546.

Mortola N, Romaniuk R, Cosentino V, Eiza M, Carfagno P, Rizzo P, … Brutti L (2019). Potential use of a poultry manure digestate as a biofertiliser: Evaluation of soil properties and Lactuca sativa growth. Pedosphere 29(1):60-69. https://doi.org/10.1016/S1002-0160(18)60057-8

Nabel MD, Schrey S, Poorter H, Koller R, Jablonowski N (2017). Effects of digestate fertilization on Sida hermaphrodita: Boosting biomass yields on marginal soils by increasing soil fertility. Biomass and Bioenergy 107:207-213. https://doi.org/10.1016/j.biombioe.2017.10.009

Nelson D, Sommers L (1996). Chapter 34 total carbon, organic carbon, and organic matter. In: Sparks DL, Page AL, Helmke PA, Loeppert RH, Soltanpour PN, Tabatabai MA, Johnston CT, Sumner ME (Eds). Methods of Soil Analysis: Part 3 Chemical Methods, 5.3. Soil Science Society of America, Inc., American Society of Agronomy, Inc. https://doi.org/10.2136/sssabookser5.3.c34

Nkoa R (2014). Agricultural benefits and environmental risks of soil fertilization with anaerobic digestates: a review. Agronomy for Sustainable Development 34:473-492. https://doi.org/10.1007/s13593-013-0196-z

Pham CH, Triolo JM, Cu TTT, Pedersen L, Sommer SG (2013). Validation and recommendation of methods to measure biogas production potential of animal manure. Asian-Australasian Journal of Animal Science 26(6):864-873. https://doi.org/10.5713/ajas.2012.12623

Paterson AH, Bowers JE, Bruggmann R (2009). The Sorghum bicolor genome and the diversification of grasses. Nature 457:551-556. https://doi.org/10.1038/nature07723

Popović V, Stevanović P, Vučković S, Ikanović J, Rajičić V, Bojović R, Jakšić S (2019). Influence of CAN fertilizer and seed inoculation with NS Nitragin on Glycine max plant on pseudogley soil type. Agriculturae Conspectus Scientificus 84(2):165-171. https://hrcak.srce.hr/221760

Popović V, Vučković S, Jovović Z, Ljubičić N, Kostić M, Rakaščan N, ... Ikanović J (2020a). Genotype by year interaction effects on soybean morpho-productive traits and biogas production. Genetika 52(3):1055-1073. https://doi.org/10.2298/GENSR1802635P

Popović V, Ljubičić N, Kostić M, Radulović M, Blagojević D, Ugrenovic V, … Ivosevic B (2020b). Genotype x environment interaction for wheat yield traits suitable for selection in different seed priming conditions. Plants 9(12):1804. https://doi.org/10.3390/plants9121804

Prask H, Szlachta J, Fugol M, Kordas L, Lejman A, Tuznik F, Tuznik F (2018). Sustainability biogas production from ensiled plants consisting of the transformation of the digestate into a valuable organic-mineral granular fertilizer. Sustainability 10(3):585. https://doi.org/10.3390/su10030585

Przygocka-Cyna K, Grzebisz W (2018). Biogas digestate - benefits and risks for soil fertility and crop quality - an evaluation of grain maize response. Open Chemistry 16:258-271. https://doi.org/10.1515/chem-2018-0027

Przygocka-Cyna K, Grzebisz W (2020). The multifactorial effect of digestate on the availability of soil elements and grain yield and its mineral profile-the case of maize. Agronomy 10:275. https://doi.org/10.3390/agronomy10020275

Rajičić V, Popović V, Perišić V, Biberdžić M, Jovović Z, Gudžić N, … Terzić D (2020b). Impact of nitrogen and phosphorus on grain yield in winter triticale grown on degraded vertisol. Agronomy 10(6):757. https://doi.org/10.3390/agronomy10060757

Rakašćan N, Popović V, Dražić G, Ikanović J, Popović S, Popović B, Milanović T (2019). Circular economy in function of obtaining the biogas. XXIII International Eco-Conference@, XIII Environmental Protection of Urban and Suburban Settlements, 25-27.09.2019. Novi Sad, pp 320-329.

Scarlat N, Fahl F, Dallemand J-F, Monforti F, Motola V (2018). A spatial analysis of biogas potential from manure in Europe. Renewable and Sustainable Energy Reviews 94:915-930. https://doi.org/10.1016/j.rser.2018.06.035

Simon T, Kunzová E, Friedlová M (2015). The effect of digestate, cattle slurry and mineral fertilization on the winter wheat yield and soil quality parameters. Plant Soil Environment 61:522-527. https://doi.org/10.17221/530/2015-PSE

Spalevic V, Barovic G, Vujacic D, Curovic M, Behzadfar M, Djurovic N, … Billi P (2020). The Impact of land use changes on soil erosion in the river basin of Miocki Potok, Montenegro. Water 12(11):2973. https://doi.org/10.3390/w12112973

Stürmer B, Pfundtner E., Kirchmeyr F, Uschnig S (2020). Legal requirements for digestate as fertilizer in Austria and the European Union compared to actual technical parameters. Journal of Environmental Management 253:109756. https://doi.org/10.1016/j.jenvman.2019.109756

Tambone F, Scaglia B, D’Imporzano G, Schievano A, Orzi V, Salati S, Adani F (2010). Assessing amendment and fertilizing properties of digestates from anaerobic digestion through a comparative study with digestated sludge and compost. Chemosphere 81(5):577-583. https://doi.org/10.1016/j.chemosphere.2010.08.034

Tapparo DC, Do Amaral AC, Radis RL, Kunz A (2019). Co-digestion of animal manure and carcasses to increase biogas generation. In: Treichel H, Fongaro G (Eds). Improving biogas production: Technological Challenges, Alternative Sources, Future Developments. Improving Biogas Production. Biofuel and Biorefinery Technologies 9. Springer Cham, pp 99-116. https://doi.org/10.1007/978-3-030-10516-7_5

Verdi L, Kuikman PL, Orlandini S, Mancini M, Napoli M, Dalla Marta A (2019). Does the use of digestate to replace mineral fertilizers have less emissions of N2O and NH3? Agricultural and Forest Meteorology 269:112-118. https://doi.org/10.1016/j.agrformet.2019.02.004

Wannasek L, Ortner M, Amon B, Amon T (2017). Sorghum, a sustainable feedstock for biogas production? Impact of climate, variety and harvesting time on maturity and biomass yield. Biomass and Bioenergy 106:137-145. https://doi.org/10.1016/j.biombioe.2017.08.031

Zegada-Lizarazu W, Monty A (2011). Energy crops in rotation: A review. Biomass and Bioenergy 35(1):12-25. https://doi.org/10.1016/j.biombioe.2010.08.001

How to Cite
POPOVIĆ, V., RAKASCAN, N., DRAZIC, G., MILOVANOVIC, J., ZIVANOVIC, L., ACIMIC REMIKOVIC, M., MILANOVIC, T., & IKANOVIC, J. (2021). Effect of digestate from anaerobic digestion on Sorghum bicolor L. production and circular economy. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12270. https://doi.org/10.15835/nbha49112270
Research Articles
DOI: 10.15835/nbha49112270