Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam.

  • Carlos G. ANDRADE Universidad Central de Venezuela, Facultad de Farmacia, Cátedra de Farmacología, Piso 7, Oficina 715, Caracas (VE)
  • Emanuel M. da SILVA University of Madeira, ISOPlexis Center for Sustainable Agriculture and Food Technology, Campus da Penteada. 9020-105 Funchal (PT)
  • Carla RAGONEZI University of Madeira, ISOPlexis Center for Sustainable Agriculture and Food Technology, Campus da Penteada. 9020-105 Funchal;Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real (PT)
  • Miguel Â. A. PINHEIRO DE CARVALHO University of Madeira, ISOPlexis Center for Sustainable Agriculture and Food Technology, Campus da Penteada. 9020-105 Funchal; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real; University of Madeira, Faculty of Life Sciences, Campus da Penteada, 9020-105 Funchal (PT)
Keywords: Carlavirus; cultivars; sweet potato; germplasm bank; molecular diagnosis; RdRp; phylogeny

Abstract

Ipomoea batatas (L.) Lam. commonly known as sweet potato, is an important staple food worldwide, mainly due to its high nutritional value and yield. However, vegetative reproduction of sweet potato makes it more susceptible to viral infections, which threatens its productivity, quality, and difficult long-term preservation in germplasm banks. Also, it can act as a virus reservoir infecting the rest of the plant accessions in the bank collections. Hence, this work aimed to screen Begomovirus, Potyvirus, and Carlavirus infections in 16 traditional sweet potato cultivars from the germplasm collection of the ISOPlexis Germplasm Bank, Madeira, Portugal. The infection prevalence by these viruses among cultivars was 81.25%, 25.00%, and 6.25%, respectively; being ISOP1011 the only accession coinfected by Potyvirus and Carlavirus. The accessions ISOP1006, ISOP1010, and ISOP1047 were also coinfected by Begomovirus and Potyvirus, highlighting their vulnerability to viral infections. The ISOP1005 and ISOP1027 accessions were the only ones not infected by any of these viruses. The analysis of the partial sequence obtained from the Carlavirus detected in the accession ISOP1011, revealed the existence of an ORF that encodes for 93 amino acids of the catalytic domain of an RNA-directed RNA polymerase related to the Tymovirus protein family, as could be confirmed by comparison with proteins stored in UniProtKB. Multiple sequence alignment with these proteins showed that Motifs A and B of the catalytic domain were conserved. The search for sequence similarity with sequences deposited in GenBank reported a high sequence identity with Sweet potato yellow mottle virus (SPYMV) and Sweet potato chlorotic fleck virus (SPCFV). However, the 9-11% discrepancy in nucleotide sequence identity and a phylogenetic analysis carried out using the maximum probability method suggests the virus isolated from ISOP1011 is a new divergent strain of the SPCFV species.

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Published
2021-03-24
How to Cite
ANDRADE, C. G., da SILVA, E. M., RAGONEZI, C., & PINHEIRO DE CARVALHO, M. Â. A. (2021). Viral diagnosis in cultivars of Ipomoea batatas (L.) Lam. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12222. https://doi.org/10.15835/nbha49112222
Section
Research Articles
CITATION
DOI: 10.15835/nbha49112222