Comparative transcriptome analysis reveals gene network regulation of TGase-induced thermotolerance in tomato

  • Mohammad S. JAHAN Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095; Sher-e-Bangla Agricultural University, Faculty of Agriculture, Department of Horticulture, Dhaka 1207 (BD)
  • Zhengrong SHI Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095 (CN)
  • Min ZHONG Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095 (CN)
  • Yuemei ZHANG Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095 (CN)
  • Ranran ZHOU Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095 (CN)
  • Mohamed M. EL-MOGY Cairo University, Faculty of Agriculture, Vegetable Crop Department, 12613 Giza (EG)
  • Jin SUN Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095; Nanjing Agricultural University, Suqian Academy of Protected Horticulture, Suqian 223800 (CN)
  • Sheng SHU Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095; Nanjing Agricultural University, Suqian Academy of Protected Horticulture, Suqian 223800 (CN)
  • Shirong GUO Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095; Nanjing Agricultural University, Suqian Academy of Protected Horticulture, Suqian 223800 (CN)
  • Yu WANG Nanjing Agricultural University, College of Horticulture, Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture, Nanjing 210095 (CN)
Keywords: high temperature; photosynthesis; tomato; transglutaminase; transcriptome

Abstract

Transglutaminase (TGase), the ubiquitous protein in plants, catalyzes the post-translational transformation of proteins and plays a vital role in photosynthesis. However, its role and mechanism in tomato subjected to heat stress still remain unknown. Here, we carried out a transcriptomic assay to compare the differentially expressed genes (DEGs) between wild type (WT) and TGase overexpression (TGaseOE) plants employed to high-temperature at 42 °C and samples were collected after 0, 6, and 12 h, respectively. A total of 11,516 DEGs were identified from heat-stressed seedlings, while 1,148 and 1,353 DEGs were up-and down-regulated, respectively. The DEGs upon high-temperature stress were closely associated with the pathways encompassing protein processing in the endoplasmic reticulum, carbon fixation, and photosynthetic metabolism. In addition, 425 putative transcription factors (TFs) were identified, and the majority of them associated with the bHLH, HSF, AP2/ERF, MYB, and WRKY families. RNA-seq data validation further confirmed that 8 genes were linked to protein processing and photosynthesis, and the mRNA level of these genes in TGaseOE was higher than that in WT plants, which is consistent in transcriptome results. In conclusion, these results reveal the transcriptional regulation between WT and TGaseOE in tomato under heat stress and shed light on a new dimension of knowledge of TGase-mediated thermotolerance mechanism at the molecular level.

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Published
2021-03-09
How to Cite
JAHAN, M. S., SHI, Z., ZHONG, M., ZHANG, Y., ZHOU, R., EL-MOGY, M. M., SUN, J., SHU, S., GUO, S., & WANG, Y. (2021). Comparative transcriptome analysis reveals gene network regulation of TGase-induced thermotolerance in tomato. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12208. https://doi.org/10.15835/nbha49112208
Section
Research Articles
CITATION
DOI: 10.15835/nbha49112208