Transcriptome-wide identification and characterization of WD40 genes, as well as their tissue-specific expression profiles and responses to heat stress in Dimocarpus longan Lour.

  • Wei ZHENG Harbin University of Commerce, School of Pharmacy, No. 138, Tongda Street, Daoli District, 150076, Harbin, (CN)
  • Ziwei ZHANG Harbin University of Commerce, School of Library, No. 1, Xuehai Street, Songbei District, 150028, Harbin (CN)
  • Xuefei YU Harbin University of Commerce, School of Library, No. 1, Xuehai Street, Songbei District, 150028, Harbin (CN)
  • Tongtong XIE Harbin University of Commerce, School of Pharmacy, No. 138, Tongda Street, Daoli District, 150076, Harbin (CN)
  • Ning CHEN Harbin University of Commerce, School of Pharmacy, No. 138, Tongda Street, Daoli District, 150076, Harbin (CN)
  • Wenlan LI Harbin University of Commerce, School of Pharmacy, No. 138, Tongda Street, Daoli District, 150076, Harbin (CN)
Keywords: bioinformatics analysis; Dimocarpus longan Lour; gene expression; WD40 gene; thermal response

Abstract

The WD40 transcription factor (TF) family is widespread in plants and plays important roles in plant growth and development, transcriptional regulation, and tolerance to abiotic stresses. WD40 TFs have been identified and characterized in a diverse series of plant species. However, little information is available on WD40 genes from D. longan. In this study, a total of 45 DlWD40 genes were identified from D. longan RNA-Seq data, and further analysed by bioinformatics tools. Also, the expression patterns of DlWD40 genes in roots and leaves, as well as responses to heat stress, were evaluated using quantitative real-time PCR (qRT-PCR). We found that the 45 DlWD40 proteins, together with 80 WD40 proteins from Arabidopsis and Zea mays, could be categorized into six groups. Of these, the DlWD40-4 protein was highly homologous to Arabidopsis WDR5a, a protein participating in tolerance to abiotic stresses. Moreover, a total of 25 cis-acting elements, such as abiotic stress and flavonoid biosynthesis elements, were found in the promoters of DlWD40 genes. The DlWD40-33 gene is targeted by miR3627, which has been proposed to be involved in flavonoid biosynthesis. Using qRT-PCR, ten of the 45 DlWD40 genes were demonstrated to have diverse expression patterns between roots and leaves, and these ten DlWD40 genes could also respond to varying durations of a 38 °C heat stress in roots and leaves. The results reported here will provide a basis for the further functional verification of DlWD40 genes in D. longan.

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Published
2021-02-25
How to Cite
ZHENG, W., ZHANG, Z., YU, X., XIE, T., CHEN, N., & LI, W. (2021). Transcriptome-wide identification and characterization of WD40 genes, as well as their tissue-specific expression profiles and responses to heat stress in Dimocarpus longan Lour. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12191. https://doi.org/10.15835/nbha49112191
Section
Research Articles
CITATION
DOI: 10.15835/nbha49112191