Morphometric analysis and sequence related amplified polymorphism determine genetic diversity in Salvia species

  • Abdul SHAKOOR Henan University, College of Environment and Planning, Kaifeng, 475004, Henan (CN)
  • Fang ZHAO Henan University, College of Environment and Planning, Kaifeng, 475004, Henan (CN)
  • Gul ZAIB Yangzhou University, Institute of Epigenetics and Epigenomics, Yangzhou (CN)
  • Wuyang LI Henan University, College of Environment and Planning, Kaifeng, 475004, Henan (CN)
  • Xincan LAN Henan University, College of Environment and Planning, Kaifeng, 475004, Henan (CN)
  • Somayeh ESFANDANI-BOZCHALOYI Shahid Beheshti University, Faculty of Life Sciences and Biotechnology, Department of Plant Sciences, Tehran (IR)
Keywords: gene flow; labiatae; morphometric analysis; Salvia; sequence-related polymorphism

Abstract

Salvia species is a member of the Lamiaceae family, and it also possesses medicinal and horticulture values. The genetic diversity was assessed through sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Salvia species were studied through a combination of morphological and molecular data. One hundred forty-five individuals related to 30 Salvia were collected in 18 provinces. A total of 157 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction (PCR) from 30 Salvia species. These bands were produced with the combinations of 10 selective primers. The total number of amplified fragments ranged from 10 to 20. The predicted unbiased heterozygosity (H) varied between 0.11 (Salvia urmiensis) and 0.31 (Salvia limbata). High Shannon’s information index was detected in Salvia limbata. The genetic similarities between 30 species are estimated from 0.46 to 0.91. Clustering results showed two major clusters. According to the SRAP (Sequence-related amplified polymorphism) markers analysis, Salvia hydrangea and Salvia sharifii had the lowest similarity. Salvia bracteata and Salvia suffruticosa were genetically dissimilar to each other. This study also detected a significant signature of isolation by distance. Present results showed that sequence-related amplified polymorphism has the potential to decipher genetic affinity between Salvia species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran.

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Published
2021-01-14
How to Cite
SHAKOOR, A., ZHAO, F., ZAIB, G., LI, W., LAN, X., & ESFANDANI-BOZCHALOYI, S. (2021). Morphometric analysis and sequence related amplified polymorphism determine genetic diversity in Salvia species. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49(1), 12153. https://doi.org/10.15835/nbha49112153
Section
Research Articles
CITATION
DOI: 10.15835/nbha49112153