Effect of salinity and drought stress on morphological and biochemical properties of two Iranian fenugreek (Trigonella foenum-graecum) populations

Afsaneh TAVANGAR; Leila  KARAMI; Mohammad  HEDAYAT; Gholamreza ABDI

doi:10.15835/nbha49212038

Authors

Afsaneh TAVANGAR Persian Gulf University, Bushehr, Department of Horticulture, College of Agriculture, 7516913817 (IR)
Leila KARAMI Persian Gulf University, Bushehr, Department of Horticulture, College of Agriculture, 7516913817 (IR)
Mohammad HEDAYAT Persian Gulf University, Bushehr, Department of Horticulture, College of Agriculture, 7516913817 (IR)
Gholamreza ABDI Persian Gulf University, Department of Biotechnology, Persian Gulf Research Institute, Bushehr, 7516913817 (IR)

DOI:

https://doi.org/10.15835/nbha49212038

Keywords:

callus induction, drought stress, fenugreek, salinity stress, trigonelin

Abstract

In this study, micro propagation of two Iranian fenugreek populations and their morphological and biochemical responses to salinity and drought stresses in in vitro culture condition were conducted using factorial experiment in a completely randomized design in three replications. Different explant type (terminal bud, cotyledon and epicotyledon explant) were cultured in MS medium contain different concentration of plant growth regulators such as kin (0, 0.5 and 1 mg / l) and 2,4-D (0.5, 1 and 2 mg / l). Murashige and Skoog (MS) medium supplemented with 1 mg/l kinetin and 2 mg/l 2,4-D showed the highest callus proliferation rate per explants in both populations. The highest amount of callus volume was obtained from the explants of the terminal bud. Proliferated calli from terminal bud explant were green and yellowish, from cotyledon were yellowish to white with soft texture, and the cotyledons were greenish and compact. The results of salinity stresses with different concentrations of sodium chloride (0, 70 and 120 mM) and drought stress with polyethylene glycol (0, 5 and 10%) showed that both stresses decreased callus growth and increased total protein, proline, catalase, peroxidase and trigonelin content in both populations. Trigonelin measurement showed that ‘Borazjan’ papulation had higher trigonelin content, in vitro, than ‘Ardestan’ papulation.

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References

Abeles FB, Biles CL (1991). Characterization of peroxidases in lignifying peach fruit endocarp. Plant Physiology 95(1):269-273. https://doi.org/10.1104/pp.95.1.269

Aebi H (1974). Methods of enzymatic analysis. Bergmeyer HU (Ed.). pp 673-677.

https://doi.org/10.1016/B978-0-12-091302-2.50032-3

Anjum SA, Xie XY, Wang LC, Saleem MF, Man C, Lei W (2011). Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research. 6(9):2026-2032. https://doi.org/10.5897/AJAR10.027

Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water stress studied. Plant and Soil 39(1):205-207. https://doi.org/10.1007/BF00018060

Berglund T, Kalbin G, Strid A, Rydstrom J, Ohlsson AB (1996). UV-B and oxidative stress induced increase in nicotinamide and trigonelline and inhibition of defensive metabolism induction by poly (ADP-ribose) polymerase inhibitor in plant tissue. FEBS Letters 380(1):188-193.

https://doi.org/10.1016/0014-5793(96)00027-0

Bitarafan Z, Asghari H, Hasanloo T, Gholami A, Moradi F (2018). Biochar effect on seed trigonelline content of fenugreek (Trigonella foenum-graceum L.) ecotypes under deficit irrigation. Journal of Medicinal and Aromatic Plants 34(1):155-165. https://doi.org/10.22092/IJMAPR.2018.115556.2147

Bradford M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3

Cho Y, Kodjoe E, Puppala N, Wood AJ (2011). Reduced trigonelline accumulation due to rhizobial activity improves grain yield in peanut (Arachis hypogaea L.). Acta Agriculturae Scandinavica, Section-Soil and Plant Sciences 61(5):395-403. https://doi.org/10.1080/09064710.2010.494614

Esam AH, Esam MA (2011). Effect of mannitol and sodium chloride on some total secondary metabolites of fenugreek calli cultured in vitro. Plant Tissue Culture & Biotechnology 21(1):35-43. https://doi.org/10.3329/ptcb.v21i1.9561

Esmaeilzadeh B, Rezaeinodehi A (2014). Increased trigonelline production by salicylic acid in fenugreek (Trigonella foenum-graecum L.) cell culture. Journal of Cell and Tissue 5(2):165-172.

Farhadi H, Azizi M, Nemati H (2014). The effect of water deficit stress on morphological characteristics and yield components of eight fenugreek landraces (Trigonella foenum-graecum L.). Research Journal of Crop Science in Arid Area 1(1):1-19. https://doi.org/10.22034/CSRAR.01.01.10

Gurel S, Gurel E, Kaya Z (2011). Callus development and indirect shoot regeneration from seedling explants of sugar beet (Beta vulgaris L.) cultured in vitro. Turkish Journal of Botany 25:25-33.

Hajihashemi Sh, Rajabpour Sh (2017). The effect of paclobutrazol and gibberellin treatments on drought stress alleviation of Stevia rebaudiana callus. Journal of Plant Process and Function 6(21):1-14. (Persian)

Heng-Moss TM, Sarath G, Baxendale FP (2004). Characterization of oxidative enzyme changes in buffalo grasses challenged by Blissus occiduus. Journal of Economic Entomology 97:1086-1095. https://doi.org/10.1093/jee/97.3.1086

Hildebrand DF, Rodriguez JG, Brown GC (1986). Peroxidative responses of leaves in two soybean genotypes injured by two spotted spider mites (Acari: Tetranychidae). Journal of Economic Entomology 79:1459-1465. https://doi.org/10.1093/jee/79.6.1459

Hall JL, Flowers TJ (1973). The effect of salt on protein synthesis in the halophyte Suaeda maritima. Planta 110:361-368. https://doi.org/10.1007/BF00387064

Hayat Sh, Hayat Q, Alyemeni MN, Wani A, SH, Pichtel J, Ahmad A (2012). Role of proline under changing environments. Plant Signaling Behavior 7(11):1456-1466. https://doi.org/10.4161/psb.21949

Jamshidi S, Lahouti M, Ganjeali A (2014). Assessment of callus growth and bioproduction of diosgeninin callus culture of Trigonella foenum-graecum L. Bulletin of Environment, Pharmacology and Life Sciences 3:191-198.

Joanna C, Magdalena S, Mirosław T (2015). Optimization of in vitro culture conditions for accumulation of diosgenin by fenugreek .Journal of Medicinal Plants Studies 3(3):22-25

Khadiga G, Elaleem A, Ahmed MM, Eldin B, Saeed AE (2014). Study of the in vitro callus induction Trigonella foenum-graecum L. from cotyledons and hypocotyls explants supplemented with various plant hormones. International Journal of Current Microbiology and Applied Sciences 3(12):486-493.

Khawar KM, Gulbitti- Onarici S, Coecue S, Erisen S, Sancak C, Ozcan S (2004). In vitro crown galls induced by Agrobacterium tumefaciens strain A281 (pTiBo542) in Trigonella foenum-graecum. Biologia Plantarum 48(3):441-444. https://doi.org/10.1023/B:BIOP.0000041100.94688.2d

Kyani A, Niknam V (2015). Comparative responses of two Trigonella species to salinity and drought stresses in vitro. Progress in Biological Sciences 5(2):233-248. https://doi.org/10.22059/PBS.2015.56041

Layegh KH, Lahouti M (2011). Comparative study of the effect of drought stress on proline variations in Salvia leriifolia in soil and in vitro culture media. Journal of Biological Sciences 4(1):105-115.

Levitt J (2015). Water, radiation, salt, and other stresses. Elsevier 2(1):622.

Mawahib EM, Lamia E, Mohammed S, Badereldin AS (2013). In vitro callus induction of fenugreek (Trigonella foenum-graecum L.) using different media with different auxins concentrations. Agriculture and Biology Journal of North America 4(3):243-251. https://doi.org/10.5251/abjna.2013.4.3.243.251

Mehrafarin A, Ghaderi A, Rezazadeh SH, Naghdi BH, Nourmohammadi G, Zand ES (2010). Bioengineering of important secondary metabolites and metabolic pathways in fenugreek (Trigonella foenumgraecu L.). Journal of Medicinal Plants 9(35):1-18.

Minorsky PV (2012). Trigonelline: a diverse regulator in plants. Plant Physiology 128(1):7 -8.

Naveen CP, Ruchi A, Sanjeev A (2014). Mannitol-induced drought stress on calli of Trigonella foenum-graecum L. var. RMt-303. Indian Journal of Experimental Biology 52:1128-1137.

Pandey R, Agarwal RM (1998). Water stress-induced change in proline contents and nitrate reductase activity in rice under light and dark condition. Physiology and Molecular Biology of Plants 4: 53-57.

Piri Kh, Nazarian F (2001). Plant tissue culture. Abu Ali Sina University Press, pp 352.

Rakmini M, Benedicate S, Vivanads DS (2004). Superoxid dismutase and catalase activities and their correlation with malondealdehyde in schizophrenic patients. Indian Journal of Clinical Biochemistry 19:114-118. https://doi.org/10.1007/BF02894268

Rezaian Sh, Lahouti M, Mahmoudzadeh H (2011). Evaluation of the effect of 2,4-D concentrations and light conditions on the rate of callus formation (Trigonella foenum-graecum L.) in vitro. Journal of Biological Sciences 3(3):107-114.

Rongjie Z, Li W, Longxing W (2010). Determination of trigonelline in Trigonella foenum-graecum L. by hydrophilic interaction chromatography. Chinese Journal of Chromatography 4:379-382. https://doi.org/10.3724/SP.J.1123.2010.00379

Salma UK, Khatun F, Bhuiyan MJH, Yasmin S, Khan TH (2016). In vitro screening for drought tolerance of some chickpea varieties in Bangladesh. Progressive Agriculture 27(2):110-118. https://doi.org/10.3329/pa.v27i2.29319

Saneoka H, Moghaieb REA, Premachandra GS, Fujita K (2004). Nitrogen nutrition and water stress effects on cell membrane stability and leaf water relations in Agrostis palustris Huds. Environmental and Experimental Botany 52:131-138. https://doi.org/10.1016/j.envexpbot.2004.01.011

Sarahi NM, Moradi B (2011). The effect of salinity on protein content, pigments, sugars and phenolic compounds in tissue culture of several species of Iranian fenugreek. Journal of Sciences, Islamic Republic of Iran 36(2):53-59.

Saravanan S, Nadarajan N (2010). Effect of media supplements on in vitro response of sesame (Sesamum indicum L.) genotypes. Research Journal of Agriculture and Biological Sciences 1(1):98-100.

Serraj R, Sinclair TR (2002). Osmolyte accumulation: Can it really help increase crop yield under drought conditions. Plant, Cell and Environment 25(2):333-341. https://doi.org/10.1046/j.1365-3040.2002.00754.x

Shibli RA, Kushad M, Yousef GG, Lila M (2007). Physiological and biochemical responses of tomato micro shoots to induced salinity stress with associated ethylene accumulation. Plant Growth Regulation 51:159-169. https://doi.org/10.1007/s10725-006-9158-7

Soheilikhah Z, Karimi N, Ghasmpour H, Zebarjadi A (2013). Effects of saline and mannitol induced stress on some biochemical and physiological parameters of Arthamus tinctorius L. varieties callus cultures. Australian Journal of Crop Science 7(12):1866-1874.

Taha H, El-Bahr M, Seif-El-Nasr M (2009). In vitro studies on Egyptian Catharanthus roseus (L.) G. Don. IV: manipulation of some amino acids as precursors for enhanced of indole alkaloids production in suspension cultures. Australian Journal of Basic and Applied Sciences 3(4):3137-3144.

Tramontano WA, Jouve D (1997). Trigonelline accumulation in salt stressed legumes and the role of other osmoregulators as cell cycle control agents. Phytochemistry 44(6):1037-1040. https://doi.org/10.1016/S0031-9422(96)00715-7

Vaezi Z, Daneshvar M, Heidari M, ChehraziM (2015). Indirect regeneration plant fenugreek (Trigonella foenum-graecum L.), with the use of plant growth regulators in vitro. Bulletin of Environment, Pharmacology and Life Sciences 4(5):103-108.

Zhang J, Nguyen HT, Blum A (1999). Genetic analysis of osmotic adjust-ment in crop plants. Experimental Botany 50 (332):291-302. https://doi.org/10.1093/jxb/50.332.291

Zia A, Rezanejad F, Safarnejad A (2010). In vitro selection for NaCl tolerance in Thymus vulgaris L. Journal of Cell and Molecular Research 2(2):86-92.