Pharmacological and ADMET-based pharmacokinetic properties of Syzygium samarangense var. parviflorum leaf extract in in vitro, in vivo and in silico models

  • Rahni HOSSAIN International Islamic University, Department of Pharmacy, Chittagong, Kumira, Chittagong-4318
  • Md A. RAHMAN University of Chittagong, Department of Biochemistry & Molecular Biology, Chittagong-4331
  • Md. K. J. RAFI University of Chittagong, Department of Biochemistry & Molecular Biology, Chittagong-4331
  • Tanvir A. SIDDIQUE University of Chittagong, Department of Biochemistry & Molecular Biology, Chittagong-4331
  • Abdullah A. NOMAN International Islamic University, Department of Pharmacy, Chittagong, Kumira, Chittagong-4318
  • Arwa MAKKI University of Jeddah, Faculty of Science, Department of Biochemistry, Jeddah 80203
  • Walla ALELWANI University of Jeddah, Faculty of Science, Department of Biochemistry, Jeddah 80203
  • Dina HAJJAR University of Jeddah, Faculty of Science, Department of Biochemistry, Jeddah 80203
  • Jitbanjong TANGPONG Walailak University, School of Allied Health Sciences, Biomedical Sciences Program, Nakhon Si Thammarat, 80160
Keywords: anthelmintic; anti-inflammatory; anxiolytic; Syzygium samarangense; thrombolytic

Abstract

This research investigated pharmacological properties mainly the anti-inflammatory, anthelmintic, thrombolytic and anxiolytic potential of methanol extract of Syzygium samarangense (MESS) var. parviflorum. Anti-inflammatory action by bovine serum albumin, egg albumin denaturation and membrane stabilization, anthelmintic by live parasites, thrombolytic by clot lysis and anxiolytic by elevated plus maze (EPM) and light and dark box (LDB) tests were measured. The four targeted pharmacological properties were further justified using the most prevalent compounds, isolated from this plant, to be undergone for their pharmacokinetic property’s analyses, sitemap analyses and lignad-receptor interactions by computational models through SwissADME and Schrödinger, 2018 softwares against PDB 6COX, 6D6T, 1JFF receptors. MESS was found to display statistically significant (P < 0.05) inhibition of Bovine Serum albumin and Egg albumin denaturation compared to reference drug diclofenac sodium. Remarkable vermicidal effect on the paralysis and death of anthelmintic parasites was observed at MESS concentration 200 mg/dL. A nondescript clot lysis of MESS compared to streptokinase was evident in in vitro thrombolytic assay. MESS increased the number of times the animal crossed from one compartment to the other and the time spent in the brightly-lit chamber of the LDB. Three-methylchalcone derivatives out of seven MESS compounds were undertaken, based on cut off value and sitemap prediction score, for further ligand-receptor binding efficiency. All these three compounds showed promising docking score, glide emodel and glide energy against PDB 6COX, 6D6T and 1DDJ, plasmin proteins demonstrating the prospects of MESS to be materialized for anti-inflammatory, anthelmintic, and thrombolytic therapeutics with further clarification.

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Published
2020-09-30
How to Cite
HOSSAIN, R., RAHMAN, M. A., RAFI, M. K. J., SIDDIQUE, T. A., NOMAN, A. A., MAKKI, A., ALELWANI, W., HAJJAR, D., & TANGPONG, J. (2020). Pharmacological and ADMET-based pharmacokinetic properties of Syzygium samarangense var. parviflorum leaf extract in in vitro, in vivo and in silico models. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(3), 1155-1175. https://doi.org/10.15835/nbha48311986
Section
Research Articles