The genotypic response towards haploid induction in Turkish onion (Allium cepa L.) germplasm
Haploid induction efficiency of unpollinated flower buds in twenty-six Turkish onion germplasm were determined on two media, Dunstan and Short medium (BDS) and Murashige and Skoog’s medium (MS). On BDS medium, 5850 flower buds were cultured and the induction rate was 2.10% and a plant survival percentage of 1.62% (2 plants). On MS medium, 5850 flower buds were cultured and 175 plantlets were induced; induction rate was 2.99% and plant survival percentage was 13.71% (24 plants). The highest number of plantlets was obtained from genotype Sanliurfa 2; 27 plantlets on BDS medium while 45 plantlets on MS medium. Flow cytometry confirmed that out of the twenty-six plants, 4 plants were haploid (15.38%), 2 mixoploid (7.69%), 18 (69.23%) diploid and 2 tetraploid (7.69%).
Alan AR, Mutschler MA, Brants A, Cobb E, Earle ED (2003). Production of gynogenic plants from hybrids of Allium cepa L. and A. roylei Stearn. Plant Science 165(6):1201-1211. https://doi.org/10.1016/S0168-9452(03)00327-3
Alan AR, Zeng H, Assani A, Shi WI, McRae HE, Murch SJ, Saxena PK (2007). Assessment of genetic stability of the germplasm lines of medicinal plant Scutellaria baicalensis Georgi (Huangqin) in long-term, in vitro maintained cultures. Plant Cell Reports 26:1345-1355. https://doi.org/10.1007/s00299-007-0332-9
Alan AR, Kaska A, Arslan E, Celebi Toprak F (2017). Turkish doubled haploid onion (A. cepa L.) lines. In: The 3rd International Symposium on EuroAsian Biodiversity. Minsk, Belarus pp 114.
Anandhan S, Chavan AA, Gopal J, Mote SR, Shelke PV, Lawande KE (2014). Variation in gynogenic potential for haploid induction in Indian shortday onions. Indian Journal of Genetics and Plant Breeding 74(4):526-528. https://doi.org/ 10.5958/0975-6906.2014.00883.9
Bohanec B, Jakse M (1999). Variations in gynogenic response among long-day onion (Allium cepa L.) accessions. Plant Cell Reports 18: 737-742. https://doi.org/10.1007/s002990050652
Campion B, Azzimonti MT, Vicini E, Schiavi M, Falavigna A (1992). Advances in haploid plant induction in onion (Allium cepa L.) through in vitro gynogenesis. Plant Science 86:97-104. https://doi.org/10.1016/0168-9452(92)90183-M
Dhatt AS, Thakur P (2014). Production of doubled haploids in onion: A review. Journal of Horticultural Sciences 9(2):107-112. https://jhs.iihr.res.in/index.php/jhs/article/view/178
Dunstan DI, Short KC (1977). Improved growth of tissue cultures of the onion (Allium cepa). Physiologia Plantarum 41:70-72. https://doi.org/10.1111/j.1399-3054.1977.tb01525.x
Fayos O, Valles MP, Garces Claver A, Mallor C, Castillo AM (2015). Doubled haploid production from Spanish onion (Allium cepa L.) germplasm: embryogenesis induction, plant regeneration and chromosome doubling. Frontiers in Plant Science 6:384. https://doi.org/10.3389/fpls.2015.00384
Gamborg OL, Miller RA, Ojima K (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50:151-158. https://doi.org/10.1016/0014-4827(68)90403-5
Geoffriau E, Kahane R, Rancillac M (1997). Variation of gynogenesis ability in onion (Allium cepa L.). Euphytica 94:34-44. https://doi.org/10.1023/A:1002949606450
Jakse M, Hirschegger P, Bohanec B., Havey MJ (2010). Evaluation of gynogenic responsiveness and pollen viability of selfed doubled haploid onion lines and chromosome doubling via somatic regeneration. Journal of the American Society for Horticultural Science 135:67-73. https://doi.org/10.21273/JASHS.135.1.67
Khar A, Kumar A, Islam S, Kumar A, Agarwal A (2018). Genotypic response towards haploid induction in short day tropical Indian onion (Allium cepa). Indian Journal of Agricultural Sciences 88 (5):709-713. http://epubs.icar.org.in/ejournal/index.php/IJAgS/article/view/80058
Mathapati GB, Kalia P, Islam S, Saini N, Kumar A, Khar A (2019). Influence of culture media and their compositions on haploid induction in Indian short-day onion. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 89:739-746. https://doi.org/10.1007/s40011-018-0990-0
Michalik B, Adamusa A, Nowak E (2000). Gynogenesis in Polish onion cultivars. Journal of Plant Physiology 156(211-216). https://doi.org/10.1016/S0176-1617(00)80308-9
Murashige T, Skoog F (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Sahinalp S, Yarali Karakan F (2019). Yerel soğan genotiplerinin haploidiye yatkınlığının belirlenmesi, [Determination of haploidy frequencies of the locally grown onion (Allium cepa L.) genotypes]. Turkish Journal of Agricultural Research 6(2):186-192. https://doi.org/10.19159/tutad.523682
Yarali F, Yanmaz R (2013). Allium türlerinin ıslahında haploidi tekniğinden yararlanma [Utilization of haploidy techniques in breeding of Allium species]. Türk Bilimsel Derlemeler Dergisi 6(2):44-50 http://derleme.gen.tr/index.php/derleme/article/view/208/206
Yarali F, Yanmaz R (2017). The effects of plant growth regulators on in vitro gynogenic embryo formation in onion (Allium cepa L.). African Journal of Biotechnology 16(40):1977-1983. https://doi.org/10.5897/AJB2017.16091
Yarali Karakan F (2019). Influence of cold pretreatments on callus and embryo inductıon in onion (Allium cepa L.) via flower bud culture. Fresenius Environmental Bulletin 28(11):8070-8075 file:///C:/Users/User/Downloads/FEB_11_2019_Pp_7674-8322%20(2).pdf
Copyright (c) 2020 Notulae Botanicae Horti Agrobotanici Cluj-Napoca
This work is licensed under a Creative Commons Attribution 4.0 International License.
Open Access Journal:
The journal allows the author(s) to retain publishing rights without restriction. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.