Molecular cloning and characterization of a lipoxygenase gene (CsLOX1) from cucumber

Yong ZHOU; Mingyuan  XU; Wei  LAI; Lianghai  CHEN; Yingui  YANG; Shiqiang LIU

doi:10.15835/nbha48411960

Authors

Yong ZHOU Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang 330045 (CN)
Mingyuan XU Jiangxi Agricultural University, College of Agronomy, Nanchang 330045 (CN)
Wei LAI Jiangxi Agricultural University, College of Agronomy, Nanchang 330045 (CN)
Lianghai CHEN Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang (CN)
Yingui YANG Jiangxi Agricultural University, College of Agronomy, Nanchang 330045 (CN)
Shiqiang LIU Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang 330045 (CN)

DOI:

https://doi.org/10.15835/nbha48411960

Keywords:

cucumber; expression pattern; lipoxygenase (LOX); transgenic Arabidopsis

Abstract

Lipoxygenases (LOXs) are non-heme iron enzymes that play crucial roles in many developmental processes during plant life, and defense responses against biotic and abiotic stresses. In this study, a lipoxygenase gene (CsLOX1) was cloned and characterized from cucumber (Cucumis sativus). The coding sequence (CDS) of CsLOX1 was 741 bp, and encoded an 878 amino-acid residue protein, which was predicted to be located in the cytoplasm. CsLOX1 contained the conserved LH2/PLAT and lipoxygenase domains, as well as the representative 38 amino acids motif [His-(X)4-His-(X)4-His-(X)17-His-(X)8-His]. Multiple sequence alignment and phylogenetic analysis indicated that CsLOX1 was closely related to other dicot 9-LOXs and posesess the essential conserved residues involved in the binding of the iron atom. Promoter analysis suggested that several development-, stress-, and hormone-related cis-acting regulatory elements were present in the promoter region of CsLOX1. The function of CsLOX1 was assessed by overexpression it in Arabidopsis, and the transgenic plants were male sterile and displayed obviously increased floral shoots. These results provide some clues for revealing the biological roles of CsLOX1 in cucumber.

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