Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

  • Shuang ZHAO Yunnan University of Chinese Medicine, College of Pharmaceutical Sciences, 1076 Yuhua Road, Kunming 650500
  • Hong TANG Yunnan University of Chinese Medicine, College of Pharmaceutical Sciences, 1076 Yuhua Road, Kunming 650500
Keywords: hairy root cultures; elicitation; valtrate production; Valeriana jatamansi

Abstract

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

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References

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Published
2020-06-30
How to Cite
ZHAO, S., & TANG, H. (2020). Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(2), 839-848. https://doi.org/10.15835/nbha48211891
Section
Research Articles