Assessing the effects of in vitro imposed water stress on pineapple growth in relation to biochemical stress indicators using polynomial regression analysis

  • Daviel GÓMEZ Universidad de Concepción, Facultad de Ingeniería, Departamento de Ingeniería Química, Laboratorio de CarboCat, Región del Bio-Bio
  • Doris ESCALANTE University of Ciego de Avila, Bioplant Center, Laboratory for Plant Breeding and Conservation of Genetic Resources, Ciego de Ávila, 69450
  • Elliosha HAJARI Plant Improvement; Agricultural Research Council-Tropical and Subtropical Crops; Private Bag X11208, Nelspruit, 1200
  • Oscar VICENTE Universitat Politècnica de València, Institute for the Preservation and Improvement of Valencian Agrodiversity (COMAV), Valencia https://orcid.org/0000-0001-5076-3784
  • . SERSHEN University of the Western Cape, Department for Biodiversity and Conservation Biology, Bellville, 7535
  • José Carlos LORENZO University of Ciego de Avila, Bioplant Center, Laboratory for Plant Breeding and Conservation of Genetic Resources, Ciego de Ávila, 69450
Keywords: Ananas comosus (L.) Merr.; biostatistics; drought; in vitro osmotic stress; mannitol; plant metabolites; temporary immersion bioreactors (TIBs)

Abstract

Knowing the mechanisms that operate under water stress in commercial crops, particularly those that can affect productivity, such as phenolic or cell wall metabolism, is becoming increasingly important in a scenario of global climate change. However, our understanding of how to analyse statistically the relationships between these commonly used biochemical markers of water stress and growth in crops like pineapple, needs to be improved. In the present work, we have addressed the question of whether polynomial regression analysis can be used to describe the influence of selected plant metabolites (chlorophylls, carotenoids, phenolics and aldehydes) on shoot biomass, in response to a mannitol-induced water stress in temporary immersion bioreactors (TIBs). Polynomial regression analysis has been applied to investigate plant stress responses in many species but is very seldom used in in vitro screening studies. Here, the relationship between biochemical indicators (x; independent variable) and shoot growth (y; dependent variable) has been characterised, with y modelled as an nth degree polynomial in x. This statistical approach accommodated for the non-linear (curvilinear) relationships between variables, and the results showed that shoot biomass was negatively, and significantly correlated with soluble phenolics, cell wall-linked phenolics and other aldehydes (characterised by “High” R2 values).

Metrics

Metrics Loading ...

References

AbdElgawad H, De Vos D, Zinta G, Domagalska MA, Beemster GT, Asard H (2015). Grassland species differentially regulate proline concentrations under future climate conditions: an integrated biochemical and modelling approach. New Phytologist 208:354-369.

Anderson C, Kohorn B (2001). Inactivation of Arabidopsis SIP1 leads to reduced levels of sugars and drought tolerance. Journal of Plant Physiology 158:1215-1219.

Avramova V, AbdElgawad H, Zhang Z, Fotschki B, Casadevall R, Vergauwen L, Knapen D, Taleisnik E, Guisez Y, Asard H, Beemster GTS (2015). Drought induces distinct growth response, protection and recovery mechanisms in the maize leaf growth zone. Plant Physiology 169:1382-1396.

Avramova V, Nagel KA, AbdElgawad H, Bustos D, DuPlessis M, Fiorani F, Beemster GT (2016). Screening for drought tolerance of maize hybrids by multi-scale analysis of root and shoot traits at the seedling stage. Journal of Experimental Botany 67:2453-2466.

Avramova V, AbdElgawad H, Vasileva I, Petrova AS, Holek A, Mariën J, Beemster GT (2017). High antioxidant activity facilitates maintenance of cell division in leaves of drought tolerant maize hybrids. Frontiers in Plant Science 8:84.

Awasthi P, Gupta AP, Bedi YS, Vishwakarma RA, Gandhi SG (2016). Mannitol stress directs flavonoid metabolism toward synthesis of flavones via differential regulation of two cytochrome p450 monooxygenases in Coleus forskohlii. Frontiers in Plant Science 7:985.

Barker AV (1999). Foliar ammonium accumulation as an index of stress in plants. Communications in Soil Science and Plant Analysis 30:167-174.

Boestfleisch C, Wagenseil NB, Buhmann AK, Seal CE, Wade EM, Muscolo A, Papenbrock J (2014). Manipulating the antioxidant capacity of halophytes to increase their cultural and economic value through saline cultivation. Annals of Botany PLANTS 6:plu046. https://doi.org/10.1093/aobpla/plu046

Boestfleisch C, Papenbrock J (2017). Changes in secondary metabolites in the halophytic putative crop species Crithmum maritimum, Triglochin maritima and Halimione portulacoides as reaction to mild salt stress. PLoS ONE 12:e0176303. https://doi.org/10.1371/journal.pone.0176303

Chao SK, Kim JE, Jong AP, Eom TJ, Kim WT (2006). Constitutive expression of abiotic stress-inducible hot pepper CaXTH3, which encodes a xyloglucan endotransglycosylase/hydrolase homolog, improves drought and salt tolerance in transgenic Arabidopsis plants. FEBS Letters 580:3136-3144.

Daquinta M, Benegas R (1997). Brief review of tissue culture of pineapple. Pineapple News 3:7-9.

Díaz-López L, Gimeno V, Simón I, Martínez V, Rodríguez-Ortega W, García-Sánchez F (2012). Jatropha curcas seedlings show a water conservation strategy under drought conditions based on decreasing leaf growth and stomatal conductance. Agricultural Water Management 105:48-56.

Escalona M, Lorenzo JC, González B, Daquinta M, Borroto C, González JL, Desjardines Y (1999). Pineapple micropropagation in temporary immersion systems. Plant Cell Reports 18:743-748.

Fernández RJ, Wang M, Reynolds JF (2002). Do morphological changes mediate plant responses to water stress? A steady state experiment with two C4 grasses. New Phytologist 155:79-88.

Galle A, Haldimann P, Feller U (2007). Photosynthetic performance and water relations in young pubescent oak (Quercus pubescens) trees during drought stress and recovery. New Phytologist 174:799-810.

Gindaba J, Rozanov A, Negash L (2004). Response of seedlings of two Eucalyptus and three deciduous tree species from Ethiopia to severe water stress. Forest Ecology and Managment 201:119-129.

Gómez D, Hernández L, Valle B, Martínez J, Cid M, Escalona M, Hernández M, Beemster GTS, Tebbe CC, Yabor L, Lorenzo JC (2017). Temporary immersion bioreactors (TIB) provide a versatile, cost-effective and reproducible in vitro analysis of the response of pineapple shoots to salinity and drought. Acta Physiologiae Plantarum 39:277.

Gupta P, Sharma S, Saxena S (2015). Biomass yield and steviol glycoside production in callus and suspension culture of Stevia rebaudiana treated with proline and polyethylene glycol. Applied Biochemistry and Biotechnology 176:863-874.

Gurmani AR, Bano A, Saleem M (2007). Effect of ABA and BA on growth and ion accumulation of wheat under salinity stress. Pakistan Journal of Botany 39:141-149.

Gurr S, McPherson J, Bowles D (1992). Lignin and associated phenolic acids in cell walls. In: Wilkinson DL (Ed). Molecular Plant Pathology. Oxford Press, Oxford, pp 51-56.

Haghighi Z, Modarresi M, Mollayi S (2012). Enhancement of compatible solute and secondary metabolites production in Plantago ovata Forsk. by salinity stress. Journal of Medical Plant Research 6:3495-3500.

Heath R, Packer J (1968). Photoperoxidation in isolated chloroplast: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125:189-198.

Hernández L, Loyola-González O, Valle B, Martínez J, Díaz-López L, Aragón C, Vicente O, Papenbrock J, Trethowan R, Yabor L, Lorenzo JC (2015). Identification of discriminant factors after exposure of maize and common bean plantlets to abiotic stresses. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 43:589-598.

Hoagland RE (1990). Alternaria cassiae alters phenylpropanoid metabolism in Sicklepod (Casia obstusifolia). Journal of Phytopathology 130:177-187.

Hüve K, Bichele I, Kaldmäe H, Rasulov B, Valladares F, Niinemets Ü (2019). Responses of Aspen Leaves to Heatflecks: Both Damaging and Non-Damaging Rapid Temperature Excursions Reduce Photosynthesis. Plants 8:145.

Ivanov Z (1989). The Agricultural Experimentation Pueblo y Educación, Havana, pp 332.

Lichtenthaler HK (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods of Enzymology 148:350-382.

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologiae Plantarum 5:473-497.

Naik PM, Al-Khayri JM (2016). Abiotic and biotic elicitors–Role in secondary metabolites production through in vitro culture of medicinal plants. In: Shanker AK, Shanker C (Eds). Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives. InTech, Al-Hassa, pp 247-277. http://dx.doi.org/10.5772/61442

Peet MM, Willits D, Gardner R (1997). Response of ovule development and post-pollen production processes in male-sterile tomatoes to chronic, sub-acute high temperature stress. Journal of Experimental Botany 48:101-111.

Porra R (2002). The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research 73:149-156.

Quan R, Shang M, Zhang H, Zhao Y, Zhang J (2004). Engineering of enhanced glycine betaine synthesis improves drought tolerance in maize. Plant Biotechnology Journal 2:477-486.

Rivelli A, De Maria S, Pizza S, Gherbina P (2012). Growth and physiological response of hydroponically grown sunflower as affected by salinity and magnesium levels. Journal of Plant Nutrition 33:1307-1323.

Runeckles V (1982). Relative death rate: a dynamic parameter describing plant response to stress. Journal of Applied Ecology 295-303.

Schachtman D, Goodger J (2008). Chemical root to shoot signaling under drought. Trends in Plant Science 13:281-287.

Selmar D, Kleinwächter M (2013). Influencing the product quality by deliberately applying drought stress during the cultivation of medicinal plants. Indian Crops Production 42:558- 566.

Silva E, Ferreira-Silva S, Viegas R, Silveira J (2010). The role of organic and inorganic solutes in the osmotic adjustment of drought-stressed Jatropha curcas plants. Environmental and Experimental Botany 69:279-285.

Vieira DA, Mesquita AC, Marinho LB, Souza Vd, Aidar SdT, Carvalho MMP (2019). Gas exchanges of melon under water stress in the Submedium region of the São Francisco River Valley. Acta Scientiarum Agronomy 41. https://doi.org/10.4025/actasciagron.v41i1.42686

Wijewardana C, Alsajri FA, Reddy KR (2019). Soybean seed germination response to in vitro osmotic stress. Seed Technology 39:143-154.

Winkel-Shirley B (2002). Biosynthesis of flavonoids and effects of stress. Current Opinion in Plant Biology 5:218-223.

Zaher-Ara T, Boroomand N, Sadat-Hosseini M (2016). Physiological and morphological response to drought stress in seedlings of ten citrus. Trees 30:985-993.

Zaker A, Sykora C, Gössnitzer F, Abrishamchi P, Asili J, Mousavi SH, Wawrosch C (2015). Effects of some elicitors on tanshinone production in adventitious root cultures of Perovskia abrotanoides Karel. Indian Crops Production 67:97-102.

Published
2020-03-31
How to Cite
GÓMEZ, D., ESCALANTE, D., HAJARI, E., VICENTE, O., SERSHEN, ., & LORENZO, J. C. (2020). Assessing the effects of in vitro imposed water stress on pineapple growth in relation to biochemical stress indicators using polynomial regression analysis. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 162-170. https://doi.org/10.15835/nbha48111844
Section
Research Articles