Seed priming with hydrogen peroxide alleviates the effects of drought stress in rice (Oryza sativa L.) seedlings

Weeraphorn  JIRA-ANUNKUL; Wattana PATTANAGUL

doi:10.15835/nbha48111829

Authors

Weeraphorn JIRA-ANUNKUL Khon Kaen University, Faculty of Sciences, Department of Biology, Khon Kaen 40002 (TH)
Wattana PATTANAGUL Khon Kaen University, Faculty of Sciences, Department of Biology, Khon Kaen 40002 (TH)

DOI:

https://doi.org/10.15835/nbha48111829

Keywords:

antioxidant enzymes; drought stress; hydrogen peroxide; rice; seed priming

Abstract

Drought stress is a major factor limiting crop growth and yield. Hydrogen peroxide (H₂O₂) is known as a signalling molecule in the plant cell in which activates multiple physiological changes that play essential roles in tolerance mechanism. This study investigated the effects of seed priming with H₂O₂ on growth, some physiological characteristics and antioxidant enzyme activities in rice seedling under drought stress. Rice (Oryza sativa L.) cv. Khao Dawk Mali 105 seeds were primed with 0 (distilled water), 1, 5, 10, and 15 mM H₂O₂ and grown for 21 days. The seedlings were subjected to drought stress by withholding water for 7 days. The results showed that priming with low concentrations of H₂O₂ improved plant growth and biomass as well as relative water content, malondialdehyde content, electrolyte leakage. Priming with H₂O₂, however, had no beneficial effect on chlorophyll content, proline and leaf total soluble sugar. Seed priming with appropriate levels of H₂O₂ also enhanced antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). It is concluded that seed priming with 2-10 mM H₂O₂, is beneficial for enhancing drought tolerance in rice seedling by increasing antioxidant capacity, which in turn reduces oxidative stress and damages to the cellular components.

References

Arnon DI (1949). Copper enzymes in isolated chloroplast. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24(1):1-15.

Baninasab B, Ghobadi C (2011). Influence of paclobutrazol and application methods on high-temperature stress injury in cucumber seedlings. Journal of Plant Growth Regulation 30(2):213-219.

Bates LS, Waldren RP, Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1):205-207.

Beauchamp C, Fridovich I (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1):276-287.

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72 (1-2):248-254.

Černý M, Habánová H, Berka M, Luklová M, Brzobohatý B (2018). Hydrogen peroxide: its role in plant biology and crosstalk with signalling networks. International Journal of Molecular Sciences 19(9):2812.

Chandlee JM, Scandalios JG (1984). Analysis of variants affecting the catalase developmental program in maize scutellum. Theoretical and Applied Genetics 69(1):71-77.

Chen K, Arora R (2013). Priming memory invokes seed stress-tolerance. Environmental and Experimental Botany 94:33-45.

Cheng J, Wang L, Zeng P, He Y, Zhou R, Zhang H, Wang Z (2017). Identification of genes involved in rice seed priming in the early imbibition stage. Plant Biology 19(1):61-69.

Cruz de Carvalho MH (2008). Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signaling & Behavior 3(3):156-65.

DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28(3):350-356.

Ellouzi H, Sghayar S, Abdelly C (2017). H2O2 Seed priming improves tolerance to salinity; drought and their combined effect more than mannitol in Cakile maritima when compared to Eutrema salsugineum. Journal of Plant Physiology 210:38-50.

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29(1):185-212.

Farooq M, Nawaz A, Chaudhary MAM, Rehman A (2017). Foliage-applied sodium nitroprusside and hydrogen peroxide improves resistance against terminal drought in bread wheat. Journal of Agronomy and Crop Science 203(6):473-482.

Gill SS, Tuteja N (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48(12):909-930.

Golizadeh SK, Mahmoodi TM, Khaliliaqdam N (2015). The effects of priming by folic acid and hydrogen peroxide on morpho-physiological traits in cannabis seeds (Cannabis sativa L.). Journal of Biodiversity and Environmental Sciences 2:407-416.

Gupta K, Sengupta A, Chakraborty M, Gupta B (2016). Hydrogen peroxide and polyamines act as double-edged swords in plant abiotic stress responses. Frontiers in Plant Science 7:1343.

Hameed A, Iqbal N (2013). Chemo-priming with mannose, mannitol and H2O2 mitigate drought stress in wheat. Cereal Research Communications 42(3):450-462.

He L, Gao Z (2009). Pretreatment of seed with H2O2 enhances drought tolerance of wheat (Triticum aestivum L.) seedlings. African Journal of Biotechnology 8(22):6151-6157.

Hossain MA, Fujita M (2013). Hydrogen peroxide priming stimulates drought tolerance in mustard (Brassica juncea L.) seedlings. Plant Gene and Trait 4(20):109-123.

Hossain MA, Bhattacharjee S, Armin S, Qian P, Xin W, Li H, … Tran LP (2015). Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Frontiers in Plant Science 6:420.

International Rice Research Institute (2002). Rice-Standard-Evaluation-System_2002. Retrieved 2019 March 1 from http://www.knowledgebank.irri.org/images/docs/rice-standard-evaluation-system.pdf

Jagodzik P, Tajdel-Zielinska M, Ciesla A, Marczak M, Ludwikow A (2018). Mitogen-activated protein kinase cascades in plant hormone signaling. Frontiers in Plant Science 6:420.

Kaur G, Asthir B (2017). Molecular responses to drought stress in plants. Biologia Plantarum 61:201-209.

Kerchev P, van der Meer T, Sujeeth N, Verlee A, Stevens CV, Van Breusegem F, Gechev T (2020). Molecular priming as an approach to induce tolerance against abiotic and oxidative stresses in crop plants. Biotechnology Advances 31:107503.

Li JT, Qiu ZB, Zhang XW, Wang LS (2011a). Exogenous hydrogen peroxide can enhance tolerance of wheat Seedlings to salt stress. Acta Physiologiae Plantarum 33(3):835-842.

Li Y, Zhao H, Duan B, Korpelainen H, Li C (2011b). Effect of drought and ABA on growth, photosynthesis and antioxidant system of Cotinus coggygria seedlings under two different light conditions. Environmental and Experimental Botany 71(1):107-113.

Lu S, Su W, Li H, Guo Z (2009). Abscisic acid improves drought tolerance of triploid bermudagrass an involves H2O2 and NO-induced antioxidant enzyme activities. Plant Physiology and Biochemistry 47:132-138.

Mishra AK, Agrawal SB (2014). Cultivar specific response of CO2 fertilization on two tropical Mung bean (Vigna radiata L.) cultivars: ROS Generation, antioxidant status, physiology, growth, yield and seed quality. Journal of Agronomy and Crop Science 200(4):273-289.

Mohammadkhani N, Heidari R (2008). Drought-induced accumulation of soluble sugars and proline in two maize varieties. World Applied Sciences Journal 3(3):448-453.

Nakano Y, Asada K (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 22(5):867-880.

Neill S, Desikan R, Hancock J (2002). Hydrogen peroxide signalling. Current Opinion in Plant Biology 5(5):388-395.

Niu L, Liao W (2016). Hydrogen peroxide signaling in plant development and abiotic responses: crosstalk with nitric oxide and calcium. Frontiers in Plant Science 7:230.

Paparella S, Araújo SS, Rossi G, Wijayasinghe M, Carbonera D, Balestrazzi A (2015). Seed priming: state of the art and new perspectives. Plant Cell Reports 34(8):1281-1293.

Quan LJ, Zhang B, Shi WW, Li HY (2008). Hydrogen peroxide in plants: A versatile molecule of the reactive oxygen species network. Journal of Integrative Plant Biology 50(1):2-18.

Savvides A, Ali S, Tester M, Fotopoulos V (2016). Chemical priming of plants against multiple abiotic stresses: mission possible?. Trends in Plant Science 21(4):329-340.

Saxena I, Srikanth S, Chen Z (2016). Cross talk between H2O2 and interacting signal molecules under plant stress response. Frontiers in Plant Science 7:570.

Smirnoff N, Arnaud D (2019). Hydrogen peroxide metabolism and functions in plants. New Phytologists 211:1197-1214.

Sun Y, Wang H, Liu S, Peng X (2016). Exogenous application of hydrogen peroxide alleviates drought stress in cucumber seedlings. South African Journal of Botany 106:23-28.

Sunohara Y, Matsumoto H (2004). Oxidative injury induced by the herbicide quinclorac on Echinochloa oryzicola Vasing. and the involvement of antioxidative ability in its highly selective action in grass species. Plant Science 167(3):597-606.

Tena G, Asai T, Chiu WL, Sheen J (2001). Plant mitogen-activated protein kinase signaling cascades. Current Opinion in Plant Biology 4(5):392-400.

Terzi R, Kadioglu A, Kalaycioglu E, Saglam A (2014). Hydrogen peroxide pretreatment induces osmotic stress tolerance by influencing osmolyte and abscisic acid levels in maize leaves. Journal of Plant Interactions 9(1):559-565.

Turner NC (1981). Techniques and experimental approaches for the measurement of plant water status. Plant and Soil 58(1-3):339-366.

Wang P, Song CP (2008). Guard-cell signalling for hydrogen peroxide and abscisic acid. New Phytologist 178(4):703-718.

You J, Chan Z (2015). ROS regulation during abiotic stress responses in crop plants. Frontiers in Plant Science 6:1092.

Zhang X, Mi X, Chen C, Wang H, Guo W (2018). Identification on mitogen-activated protein kinase signaling cascades by integrating protein interaction with transcriptional profiling analysis in cotton. Scientific Reports 8(1):1-14.