Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities
Keywords:antioxidant properties; chemical composition; extraction; solvent
The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.
Adams RP (2007). Identification of essential oil component by gas chromatography/ mass spectrometry. Allured Publishing Corporation, Carol Stream, Illinois.
Agili FA (2014). Chemical composition, antioxidant and antitumor activity of Thymus vulgaris L. essential oil. Middle-East Journal of Scientific Research 21(10):1670-1676.
Aksay S (2016). Total phenolic content and antioxidant properties of various extracts of myrtle (Myrtus communis L.) berries. Cukurova Journal of Agriculture and Food Sciences 31:43-50.
Alia NAA, Wursterb M, Denkertc A, Arnoldc N, Fadaila I, Al-Didamonya G, … Setzerd WN (2012). Chemical composition, antimicrobial, antioxidant and cytotoxic activity of essential oils of Plectranthus cylindraceus and Meriandra benghalensis from Yemen. Natural Product Communications 7:1099-1102.
Alizadeh A, Alizadeh O, Amari G, Zare M (2013). Essential oil composition, total phenolic content, antioxidant activity and antifungal properties of Iranian Thymus daenensis subsp. daenensis Celak. as in influenced by ontogenetical variation. Journal of Essential Oil Bearing Plants 16(1):59-70.
Amensour M, Sendra E, Abrini J, Bouhdid S, Pérez-Alvarez J, Fernández-López J (2009). Total phenolic content and antioxidant activity of myrtle (Myrtus communis) extracts. Natural Products Communications 4:819-824.
Bidgoli RD, Ebrahimabadi A, HaghighiQamsari N, Batooli H (2019). Essential oil composition and biological acivities of extracts of Acinos graveolens from Iran. Life Science Informatics Publications 5:834-844.
Carović-Stanko K, Petek M, Grdiša M, Pintar J, Bedeković D, Herak Ćustić M, Satovic Z (2016). Medicinal plants of the family Lamiaceae as functional foods- a review. Czech Journal of Food Sciences 34:377-390.
Chalchat JC, Maksimovic ZA, Petrovic SD, Gorunovic MS (2004). Essential oil of Acinos hungaricus (Simonkai) Silic, Lamiaceae. Journal of Essential Oil Research 16:38-39.
Couladis M, Tzakou O, Demetzos C, Perdetzoglou D (2002). Chemical composition and antibacterial activity of the oil of Acinos suaveolens (Sibth. et Sm.) G. Don f. from Greece. Journal of Essential Oil Research 14:139-140.
Cuendet M, Hostettmann K, Potterat O (1997). Iridoid glucosides with free radical scavenging properties from Fagraea blumei. Helvetica Chimica Acta 80:1144-1152.
Do Q, Angkawijaya A, Tran-Nguyen P, Huynh L, Soetaredjo F, Ismadji S, Ju YH (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic. Journal of Food and Drug Analysis 22:296-302.
European Pharmacopeia (2008). Council of Europe, Strasbourg. 6.0, 5th edition.
German Pharmacopoeia DAB 10 (1996). Deutsches Arzneibuch. 10th Edition. 3rd suppl. Deutscher Apotheker Verlag, Stuttgart.
Golubovic T (2010). Secondary metabolites of selected species of the genus Acinos Miller. PhD Thesis, University of Niš, Niš.
Golubovic T, Palic R, Kitic D, Zlatkovic B, Ristic M, Lazarevic J, Stojanovic G (2010). Chemical composition and antimicrobial activity of the essential oil of Acinos graveolens. Chemistry of natural compounds 46:645-648.
Golubovic T, Palic R, Kitic D, Stojanovic G, Zlatkovic B, Ristic M, Pavlovic D (2014). Composition, antioxidant and antimicrobial activities of methanol extracts of some Acinos Miller species. Natural Products Communications 9:731-735.
Hagerman A, Mueller-Harvey I, Makkar HPS (2000). Quantification of tannins in tree foliage-a laboratory manual. FAO/IAEAWorking document, IAEA, Vienna, pp 4-6.
Ho CL, Su YC (2012). Composition, antioxidant and antimicrobial activities of the leaf essential oil of Machilus japonica from Taiwan. Natural Product Communications 7:109-112.
Hussain A, Chatha S, Noor S, Arshad M, Khan Z, Rathore H, Sattar M (2011). Effect of extraction techniques and solvent systems on the extraction of antioxidant components from peanut (Arachis hypogaea L.) hulls, Food analytical methods. DOI 10.1007/s12161-011-9325-y
Jovanovic T, Palic R, Stojanovic G, Ristic M, Kitic D (2002). Chemical composition of essential oil of Acinos hungaricus (Simonkai) Silic. Journal of Essential Oil Research 14:29-30.
Jovanovic T, Palic R, Stojanovic G, Ristic M, Kitic D (2005). Chemical composition and antimicrobial activity of the essential oil of Acinos arvensis (Lam.) dandy from Serbia. Flavour and Fragrance Journal 20:288-290.
Jovanovic T, Palic R, Kitic D, Ristic M, Zlatkovic B (2008). Fatty acids of Acinos alpinus and A. hungaricus. Chemistry of Natural Compounds 44:231-233.
Kaya A, Bacer K, Tumen G, Koca F (1999). The essential oil of Acinos suaveolens (Sm.) G. Don fil, Acinos arvensis (Lam.) dandy and Acinos rotundifolius Pers. Moench growing in Turkey. Flavour and Fragrance Journal 14:60-64.
Kaya A, Baser KHC, Demirci B, Koca F (1999). The essential oil of Acinos alpinus (L.) Moench growing in Turkey. Flavour and Fragrance Journal 14:55-59.
Kaya A, Baser KHC, Koca F (1999). Essential oils of Acinos troodi (Post) Leblebici subsp. vardaranus Leblebici and subsp. grandiflorus Hartvig & Strid. Flavour and Fragrance Journal 14:50-54.
Karousou R, Balta M, Hanlidou E, Kokkini S (2007). "Mints", smells and traditional uses in Thessaloniki (Greece) and other Mediterranean countries. Journal of Ethnopharmacology 109:248-257.
Kokkalou E (1988). Composition of the volatile oil from Acinos suaveolens. Planta Medica 54:340-342.
Kostic D, Mitic S, Mitic M, Zarubica A, Velickovic J, Dordevic A, Randelovic S (2010). Phenolic contents, antioxidant and antimicrobial activity of Papaver rhoeas L. extracts from Southeast Serbia. Journal of Medicinal Plants Research 4:1727-1732.
Kamkar A, Tooriyan F, Jafari M, Bagherzade M, Saadatjou S, Aghaee E (2014). Antioxidant activity of methanol and ethanol extracts of Satureja hortensis L. in soybean oil. Journal of Food Quality and Hazards Control 1:113-119.
Kordali S, Cakir A, Ozer H, Cakmakci R, Kesdek M, Mete E (2008). Antifungal, phytotoxic and insecticidal properties of essential oil isolated from Turkish Origanum acutidens and its three components, carvacrol, thymol and p-cymene. Bioresource Technology 99:8788-8795.
Kulisic T, Radonic A, Katalinic V, Milos M (2004). Use of different methods for testing antioxidative activity of oregano essential oil. Food Chemistry 85:633-640.
Mathela CS, Singh KK, Gupta VK (2010). Synthesis and in vitro antibacterial activity of thymol and carvacrol derivatives. Acta Poloniae Pharmaceutica-Drug Research 67:375-380.
Mihajlov-Krstev TM, Kitic D, Radnovic DV, Ristic MS, Mihajlovic-Ukropina M, Zlatkovic BK (2011). Chemical composition and antimicrobial activity of Satureja kitaibelii essential oil against pathogenic microbial strains. Natural Product Communications 6:1167-1172.
Nsor-Atindana J, Zhong F, Mothibe K, Bangoura M, Lagnika C (2012). Quantification of total polyphenolic content and antimicrobial activity of cocoa (Theobroma cacao L.) Bean Shells. Pakistan Journal of Nutrition 11:574-579.
Pavlovic S, Kuznjecova G, Zivanovic P, Sevarda A, Jancic R, Vujcic S (1983). Content and composition of essential oil and some anatomical characteristics of plants of the species Acinos suaveolens (Sibth and SM) G. Don fil. Arhiv za Farmaciju 34:65-71.
Pavlovic S, Zivanovic P, Jancic R, Kuznjecova G, Vujcic S, Sevarda A (1983). Study of the species Acinos majoranifolius (Mill.) Silic (Lamiaceae) from Orjen Mountain, as a new source of essential oils. Arhiv za Farmaciju 34:27-33.
Performance Standards for Antimicrobial Disk Susceptibility Test, 6th edition (1997). National Committee for Clinical Laboratory Standards, approved standard: PA Wayne, M100-S9.
Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, Brighenti F (2003). Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. Journal of Nutrition 133:2812-2819.
Shan B, Cai YZ, Sun M, Corke H (2005). Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. Journal of the Agricultural and Food Chemistry 53:7749-7759.
Skaltsa HD, Lazaris DM, Loukis AE (1999). Composition of the essential oil of Acinos alpinus (L.) Moench. from Greece. Journal of Essential Oil Research 11:35-37.
Stojanovic G, Golubovic T, Kitic D, Palic R (2009). Acinos species: Chemical composition, antimicrobial and antioxidative activity. Journal of Medicinal Plants Research 3:1240-1247.
Stojković M, Mitić S, Pavlović J, Stojanović B, Paunović D (2014). Antioxidant potential of Tanacetum vulgare L. extracts. Biologica Nyssana 5:47-51.
Souleles C, Katsiotis S (1988). Study on the essential oil of Acinos arvensis (Lam.) Dandy. Plantes Medicinales et Phytotherapie 22:180-183.
Sultana B, Anwar F, Ashraf M (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules 14:2167-2180.
Thompson J, Chalcha J, Michet A, Linhart Y, Ehlers B (2003). Qualitative and quantitative variation in monoterpene co-occurrence and composition in the essential oil of Thymus vulgaris chemotypes. Journal of Chemical Ecology 29:859-880.
Triantaphyllou K, Blekas G, Boskou D (2001). Antioxidative properties of water extracts obtained from herbs of the species Lamiaceae. International Journal of Food Sciences and Nutrition 52:313-317.
Truong DH, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC (2019). Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. Journal of Food Quality 2019(1):1-9.
Tumen G (1991). The volatile constituents of Acinos suaveolens (Sibt.et Smith) G. Don fil. growing in Turkey. Journal of Essential Oil Research 3:191-192.
Ulukanli Z, Ulukanli S, OzbayH, Ilcim A, Tuzcu M (2005). Antimicrobial activities of some plants from the Eastern Anatolia region of Turkey. Pharmaceutical Biology 43:334-339.
Velasco-Negueruela A, Perez-Alonso MJ, Jimenez SM, Garcia FM (1993). The volatile constituents of Acinus alpinus (L.) Moench ssp. meridionalis (Nyman) P. W. Ball growing in Spain. Flavour and Fragrance Journal 8:127-130.
Wojdylo A, Oszmianski J, Czemerys R (2007). Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry 105:940-949.
Zlatković B, Ranđelović V (2004). Records of new species to the flora of Serbia. Abstracts of XI OPTIMA Meeting, Belgrade pp 66.
Šilić Č (1979). The Monograph of genus Satureja L., Calamintha Miller, Micromeria Bentham, Acinos Miller and Clinopodium L. in Flora of Yugoslavia. Svijetlost, Sarajevo.
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