Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts

  • Saliha EKŞİ Recep Tayyip Erdoğan University, The Faculty of Medicine, Department of Medical Microbiology, 53020 Rize
  • Ülkü Zeynep ÜREYEN ESERTAŞ Recep Tayyip Erdoğan University, The Faculty of Medicine, Department of Medical Microbiology, 53020 Rize
  • Ali Osman KILIÇ Karadeniz Technical University, Faculty of Medicine, Department of Medical Microbiology, 61080 Trabzon
  • Nebahat EJDER Recep Tayyip Erdoğan University, The Faculty of Medicine, Department of Medical Microbiology, 53020
  • Barış UZUNOK Uşak University, Faculty of Medicine, Department of Physiology, 64300 Uşak
Keywords: antimicrobial activity; biofilms; Castanea sativa; quorum sensing

Abstract

The rapid rise of resistance causes existing antibiotics to become dysfunctional. Therefore, search for new antimicrobial active ingredients has increased in recent years. In this study, flower extracts of Castanea sativa were examined for antimicrobial and anti-quorum sensing aspects. The antimicrobial properties of methanol, ethyl acetate, ethanol and hexane extracts of C. sativa against some gram-positive and gram-negative bacterial species, as well as yeasts (Candida albicans and Candida parapsilosis) were investigated by the agar well diffusion method. The minimum inhibition concentration (MIC) and minimum bactericidal concentrations (MBC) of C. sativa extracts were also determined. Chromobacterium violaceum ATCC 12472, C. violaceum 35352, C. violaceum VIR07 and C. violaceum CV026 indicator strains were used for determination of the quorum sensing inhibitions, and the Pseudomonas aeruginosa PAO1 strain was used for the swarming tests. Additionally, biofilm inhibition was detected by the spectrophotometric method using the P. aeruginosa PAO1 strain. Methanol, ethanol and ethyl acetate extracts of C. sativa was found to have high antibacterial and antifungal effects, while the methanol extract also had anti-quorum sensing, anti- swarming and biofilm inhibition effects, but no activity was found in the n- hexane extract. To the best of our knowledge this is the first report revealed that methanol extract obtained from C. sativa flowers induced anti-quorum sensing activities mainly inhibited the violacein production, swarming and biofilm formation. The present investigation provided evidence that the C. sativa flower extract maybe a potential source of antimicrobial agents. Therefore, much attention should be paid to C. sativa flower content, which could be used with high efficacy against microorganisms.

Metrics

Metrics Loading ...

References

Avşar C, Özler H, Berber İ, Civek S (2016). Phenolic composition, antimicrobial and antioxidant activity of Castanea sativa Mill. pollen grains from Black Sea Region of Turkey. International Food Research Journal 23(4):1711-1716.

Bacha K, Tariku Y, Gebreyesus F, Zerihun S, Mohammed A, Weiland-Bräuer N, Mulat M (2016). Antimicrobial and anti-quorum sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. BMC Microbiology 16(1):139.

Best CA and Best TJ (2009). Mycobacterium smegmatis infection of the hand. International Journal of Biomedical and Advance Research Case Reports 4(2):165-166.

Carocho M, Barros L, Bento A, Santos-Buelga C, Morales P, Ferreira ICFR (2014). Castanea sativa Mill. flowers amongst the most powerful antioxidant matrices: a phytochemical approach in decoctions and infusions. Biomed Research International 2014.

Clinical and Laboratory Standards Institute (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically approved standards. Seventh Edition Document, CLSI document M7- A7 Wayne, PA/USA.

Clinical and Laboratory Standards Institute (2008). Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard, 3rd ed., CLSI document M27-A3, Wayne, PA/USA.

Clinical and Laboratory Standards Institute (2009). Method for antifungal disk diffusion susceptibility testing of yeasts; approved guideline, 2nd ed., CLSI document M44-A2, Wayne, PA/USA.

Cowan MM (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews 12 (4):564-582.

Denev P, Kratchanova M, Ciz M, Lojek A, Vasicek O, Nedelcheva P, … Vojtek L (2014). Biological activities of selected polyphenol-rich fruits related to immunity and gastrointestinal health. Food Chemistry 157:37-44.

Fokou VT, Twum Kissi AD, Manu Yeboah D (2016). In vitro activity of selected west African medicinal plants against Mycobacterium ulcerans disease Patrick. Molecules 21(4):445.

Hamidi S (2013). Bazı Sentetik Kimyasal Maddelerin ve Doğal Ekstraktların Antimikrobiyal ve Anti- quorum sensing aktivitelerinin Araştırılması. MSc Dissertation, Karadeniz Technical University, Trabzon.

Hao JJ, Liu H, Donis-Gonzalez IR, Lu HX (2012). Antimicrobial activity of chestnut extracts for potential use in managing soilborne plant pathogens. Plant Disease 96(3):354-360.

Houghton PJ, Raman A (1998). Laboratory handbook for the fractionation of natural extracts. Department of Pharmacy King’s College London UK pp 14-21.

Ketenoğlu O, Tug GN, Kurt L (2010). An ecological and syntaxonomical overview of Castanea sativa and a new association in Turkey. Journal of Environmental Biology 31(1-2):81-6.

Koh CL, Sam CK, Yin WF, Tan LY, Krishnan T, Chong YM, Chan KG (2013). Plant-derived natural products as sources of anti-quorum sensing compounds. Sensors 13(5):6217-6228.

Koh KH, Tham FY (2011). Screening of traditional Chinese medicinal plants for quorum-sensing inhibitors activity. Journal of Microbiology Immunology and Infection. 44(2):144-148.

Kohler PP, Volling C, Green K, Uleryk EM, Shah PS, McGeer A (2017). Carbapenem resistance, initial antibiotic therapy, and mortality in Klebsiella pneumoniae bacteremia: a systematic review and meta-analysis. Infection Control and Hospital Epidemiology 38(11):1319-1328.

Kolaylı S, Can Z, Yıldız O, Şahin H, Alpay Karaoğlu Ş (2016). A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys. Journal of Enzyme Inhibition and Medicinal chemistry 31(3):96-104.

Li CF, Zhu Y, Yu Y (2015). Global transcriptome and gene regulation network for secondary metabolite biosynthesis of tea plant (Camellia sinensis). BMC Genomics 16(1):560.

Lim TK (2013). Edible medicinal and non-medicinal plants. Fruits. Academic Press (6th ed), New York.

McLean RJC, Pierson LS, Fuquac C (2004). A simple screening protocol for the identification of quorum signal antagonist. Journal of Microbiological Methods 58(3):351-360.

Mcloone P, Warnock M, Fyfe L (2015). Honey: a realistic antimicrobial for disorders of the skin. Journal of Microbiology, Immunology and Infection 49(2):161-167.

Murray PR, Baron EJ, Pfaller MA, Jorgensen JH, Yolken RH (2009). Manual of clinical microbiology. Academic Press (9 th ed), Washington.

National Committee for Clinical Laboratory Standart (1999). Methods for determining bacterial activity of antimicrobial agentes; Approved Guideline. NCCLS, M26-A,19, Willanova, PA/USA.

Newman DJ and Cragg GM (2012). Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of Natural Products 75(3):311-335.

Niu H, Zhang W, Wei L, Liu H, Zhao C, Liu M, Geng J (2019). Rapid nanopore assay for carbapenem-resistant Klebsiella pneumonia. Frontiers Microbiology 10:1672.

Pacheco-Ordaz R, Wall-Medrano A, Goni MG, Ramos-Clamont-Montfort G, Ayala Zavala JF, Gonzzalez-Aguilar GA (2018). Effect of phenolic compounds on the growth of selected probiotic and pathogenic bacteria. Letters in Applied Microbiology 66(1):25-31.

Paul GG, Gordon MC, David JN (2010). Plant natural products in anticancer drug discovery. Current Organic Chemistry 14(16):1781-1791.

Pereira C, Barros L, Carvalho AM, Ferreira ICFR (2013). Use of UFLC-PDA for the analysis of organic acids in thirtyfive species of food and medicinal plants. Food Analytical Methods 6(5):1337-1344.

Quave LC, Lyles TJ, Kavanaugh SJ, Nelson K, Parlet CP, Crosby HA, ... Horswill AR (2015). Castanea sativa (European chestnut) leaf extracts rich in ursene and oleanene derivatives block Staphylococcus aureus virulence and pathogenesis without detectable resistance. Plos One 10(8):e0136486.

Quave LC, Plano RWL, Bennett CB (2011). Quorum sensing ınhibitors for Staphylococcus aureus from Italian medicinal plants. Planta Medica 77(2):188-195.

Rashid MH, Kornberg A (2000). Inorganic polyphosphate is needed for swimming, swarming, and twitching motilities of Pseudomonas aeruginosa. Proceedings of the National Academy Sciences of the United States of America 97(9):4885-4890.

Ristic MD, Duletic-Lausevic S, Knezevic-Vukcevic J, Marin PD, Simic D, Vukojevic J, ... Vajs V (2000). Antimicrobial activity of essential oils and ethanol extract of Phlomis fruticosa L.(Lamiaceae). Phytotheraphy Research 14(4):267-271.

Ronsisvalle S, Lissandrello E, Fuochi V, Petronio GP, Straquadanio C, Crascì L, … Furneri PM (2017). Antioxidant and antimicrobial properties of Casteanea sativa Miller chestnut honey produced on Mount Etna (Sicily). Natural Product Research 33(6):843-850.

Solanki R, Nagori BP (2012). New method for extracting phytoconstituents from plants. International Journal of Biomedical and Advance Research 3(10):770-774.

Torres M, Romero M, Prado S, Dubert J, Tahrioui A, Otero A, Llamas I (2013). N-acyl homoserine lactone degrading bacteria isolated from hatchery bivalvelarval cultures. Microbiological Research 168(9):547-554.

Truchado P, Gil-Izqierdo A, Tomas-Barberan F, Allenda A (2009). Inhibition by chenut honey of N-Acyl-L-homoserine lactones and biofilm formation in Erwinia carotovora, Yersinia enterocolitica and Aeromonas hydrophila. Journal of Agricultural and Food Chemistry 57(23):11186-11193.

Uğurlu A (2013). Pseudomonas aeruginosa Suşlarında Çevreyi Algılama Sistemine Fenolik Maddelerin Etkisinin Araştırılması. Master thesis, Marmara University. İstanbul.

Vasconcelos MCBM, Bennett RN, Rosa EAS, Ferreira-Cardoso JV (2010). Composition of European chestnut (Castanea sativa Mill.) and association with health effects: fresh and processed products. Journal of the Science Food and Agriculture 90(10):1578-1589.

Woods GL, Brown-Elliott BA, Desmond EP, Hall GS, Heifets L, Pfyffer GE, … Witebsky FG (2003). Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes. Approved Standard. NCCLS Doc. M24-A, 18, pp 20-31.

Yaldız G, Yüksel T, Şekeroğlu N (2010). Rize ili florasında bulunan tıbbi ve aromatik bitkiler ve kullanım alanları [Medicinal and aromatic plants in the flora of Rize province and their usage areas]. 20-22 May 2010, III Ulusal Karadeniz Ormancılık Kongresi 3:1100-1114.

Zitkovic J, Zekovic Z, Mujic I (2010). Scavenging capacity of superoxide radical and screening of antimicrobial activity of Castanea sativa Mill. extracts. Czech Journal of Food Sciences 28(1):61-68.

Published
2020-03-31
How to Cite
EKŞİ, S., ÜREYEN ESERTAŞ, Ülkü Z., KILIÇ, A. O., EJDER, N., & UZUNOK, B. (2020). Determination of the antimicrobial and antibiofilm effects and ‘Quorum Sensing’ inhibition potentials of Castanea sativa Mill. extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(1), 66-78. https://doi.org/10.15835/nbha48111736
Section
Research Articles

Most read articles by the same author(s)