Expression of thaumatin, a new type of alternative sweetener in rice

  • Shahina AKTER Hankyong National University, Department of Horticultural Life Science, Anseong 17579; Gachon University, Department of Food Science and Biotechnology, College of BioNano Technology, Seongnam 461-701
  • Md. Amdadul HUQ Chung-Ang University, Department of Food and Nutrition, College of Biotechnology and Natural Resource, Anseong, Gyeonggi 17546
  • Yu-Jin JUNG Hankyong National University, Department of Horticultural Life Science, Anseong 17579; Hankyong National University, Institute of Genetic Engineering, Anseong, 17579
  • Kwon-Kyoo KANG Hankyong National University, Department of Horticultural Life Science, Anseong 17579; Hankyong National University, Institute of Genetic Engineering, Anseong, 17579
Keywords: Agrobacterium tumefaciens; Oryza sativa L; protein expression; taqman PCR; thaumatin

Abstract

  Sweet proteins are the natural alternative to the artificial sweeteners as well as flavor enhancers. Among other sweet protein, thaumatin protein was isolated from Thaumatococcus daniellii Benth plant fruit. In this study, pinII Ti plasmid vector was constructed with thaumatin gene, where thaumatin was placed under the control of the duel cauliflower mosaic virus 35S promoter into rice (Oryza sativa L. var. japonica cv. ‘Dongjinbyeo’) by Agrobacterium-mediated transformation to generate transgenic plants. Thirteen plant lines were regenerated and the transgenic rice lines were confirmed by different molecular analysis. The genomic PCR result revealed that all of the plant lines were transgenic. The single copy and intergenic plant lines were selected by Taqman PCR analysis and FST analysis, respectively. Expression of thaumatin gene in transgenic rice resulted in the accumulation of thaumatin protein in the leave. Thaumatin protein was also accumulated in leave of T1 generation. Sensory analysis result suggested that the thaumatin protein expressing transgenic lines exerted sweet tasting activity. These results demonstrated that thaumatin was expressed in transgenic rice plants.

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Published
2020-08-25
How to Cite
AKTER, S., HUQ, M. A., JUNG, Y.-J., & KANG, K.-K. (2020). Expression of thaumatin, a new type of alternative sweetener in rice. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(3), 1276-1291. https://doi.org/10.15835/nbha48311676
Section
Research Articles