Root growth of Eleuthero (Eleutherococcus senticosus [Rupr. & Maxim.] Maxim.) seedlings cultured with chitosan oligosaccharide addition under different light spectra

Shenglei  GUO; Shuang  ZHANG; Liwei  JIA; Mingyuan  XU; Zhenyue  WANG

doi:10.15835/nbha48211634

Authors

Shenglei GUO Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040 (CN)
Shuang ZHANG Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040 (CN)
Liwei JIA Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040 (CN)
Mingyuan XU Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040 (CN)
Zhenyue WANG Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040 (CN)

DOI:

https://doi.org/10.15835/nbha48211634

Keywords:

artificial illumination; Ciwujia; natural product; Northeast China; underground organ

Abstract

Eleuthero (Eleutherococcus senticosus [Ruprecht & Maximowicz] Maximowicz is an important understory species as well as a source of natural products in Asia. The natural source of Eleuthero has been over exploited for pharmacological use, but the manual restoration to rehabilitate its natural distribution has not yet been established. The root is one of the main underground organs in Eleuthero for the acquisition of pharmaceutical ingredients. Root growth also determines the seedling quality of this species for ecological restoration. In this study, Eleuthero seedlings were cultured for one growing cycle with chitosan oligosaccharide (CO) addition at rates of 0 and 25 ppm under artificial lightings by high-pressure sodium (HPS) lamps and two light-emitting diodes (LEDs) with photosynthetic photon flux density set to 94±5 μmol m^-2. Light spectra of red (R): green (G): blue (B) ratios were measured to be 43.7:54.6:1.7, 43.8:47:9.2, and 72.7:13.3:14 for the HPS, LED-1, and LED-2 treatments, respectively. In autumn, no interactive effect was found between CO addition and light spectra treatments on any root parameters. Compared to the HPS treatment, the LED-1 treatment resulted in a greater root dry weight and morphology by over 30%. The CO addition caused an increase of root growth by 40-70%. Fine roots in diameter between 0-0.4 mm were the main root part that contributed to the length, surface area, and root tip number. Therefore, LED lighting with the R/G/B spectrum of 43.8:47:9.2 was suggested for the culture of Eleuthero seedlings with the purpose of promoting dry weight and morphology of fine roots.

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