Root growth of Eleuthero (Eleutherococcus senticosus [Rupr. & Maxim.] Maxim.) seedlings cultured with chitosan oligosaccharide addition under different light spectra

  • Shenglei GUO Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040
  • Shuang ZHANG Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040
  • Liwei JIA Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040
  • Mingyuan XU Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040
  • Zhenyue WANG Laboratory of Chinese Medicine Resource, Heilongjiang University of Chinese Medicine, Harbin 150040
Keywords: artificial illumination; Ciwujia; natural product; Northeast China; underground organ


Eleuthero (Eleutherococcus senticosus [Ruprecht & Maximowicz] Maximowicz is an important understory species as well as a source of natural products in Asia. The natural source of Eleuthero has been over exploited for pharmacological use, but the manual restoration to rehabilitate its natural distribution has not yet been established. The root is one of the main underground organs in Eleuthero for the acquisition of pharmaceutical ingredients. Root growth also determines the seedling quality of this species for ecological restoration. In this study, Eleuthero seedlings were cultured for one growing cycle with chitosan oligosaccharide (CO) addition at rates of 0 and 25 ppm under artificial lightings by high-pressure sodium (HPS) lamps and two light-emitting diodes (LEDs) with photosynthetic photon flux density set to 94±5 μmol m-2. Light spectra of red (R): green (G): blue (B) ratios were measured to be 43.7:54.6:1.7, 43.8:47:9.2, and 72.7:13.3:14 for the HPS, LED-1, and LED-2 treatments, respectively. In autumn, no interactive effect was found between CO addition and light spectra treatments on any root parameters. Compared to the HPS treatment, the LED-1 treatment resulted in a greater root dry weight and morphology by over 30%. The CO addition caused an increase of root growth by 40-70%. Fine roots in diameter between 0-0.4 mm were the main root part that contributed to the length, surface area, and root tip number. Therefore, LED lighting with the R/G/B spectrum of 43.8:47:9.2 was suggested for the culture of Eleuthero seedlings with the purpose of promoting dry weight and morphology of fine roots.


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Aleksandrowicz-Trzcińska M, Bogusiewicz A, Szkop M, Drozdowski S (2015). Effect of chitosan on disease control and growth of Scots pine (Pinus sylvestris L.) in a forest nursery. Forests 6:3165-3176.

Apostol KG, Dumroese RK, Pinto JR, Davis AS (2015). Response of conifer species from three latitudinal populations to light spectra generated by light-emitting diodes and high-pressure sodium lamps. Canadian Journal of Forest Research 45:1711-1719.

Chamnanmanoontham N, Pongprayoon W, Pichayangkura R, Roytrakul S, Chadchawan S (2015). Chitosan enhances rice seedling growth via gene expression network between nucleus and chloroplast. Plant Growth Regulation 75:101-114.

Duan J, Xu CY, Jacobs DF, Ma LY, Wei HX, Jiang LN, Ren J (2013). Exponential nutrient loading shortens the cultural period of Larix olgensis seedlings. Scandinavian Journal of Forest Research 28(5):409-418.

Guo SL, Wei HX, Li JP, Fan RF, Xu MY, Chen X, Wang ZY (2019). Geographical distribution and environmental correlates of eleutherosides and isofraxidin in Eleutherococcus senticosus from natural populations in forests at Northeast China. Forests 10(10): 872.

Khan WM, Prithiviraj B, Smith DL (2002). Effect of foliar application of chitin and chitosan oligosaccharides on photosynthesis of maize and soybean. Photosynthesis 40(4):621-624.

Jeong GT, Park DH (2005). Enhancement of growth and secondary metabolite biosynthesis: Effect of elicitors derived from plants and insects. Biotechnology and Bioprocess Engineering 10:73-77.

Li XW, Gao Y, Wei HX, Xia HT, Chen QX (2017a). Growth, biomass accumulation and foliar nutrient status in fragrant rosewood (Dalbergia odorifera TC Chen) seedlings cultured with conventional and exponential fertilizations under different photoperiod regimes. Soil Science and Plant Nutrition 63(2):153-162.

Li Y, Xin GF, Min W, Shi QH, Yang FJ, Wang XF (2017b). Carbohydrate accumulation and sucrose metabolism responses in tomato seedling leaves when subjected to different light qualities. Scientia Horticultae 225:490-497.

Li XW, Chen QX, Lei HQ, Wang JW, Yang S, Wei HX (2018). Nutrient uptake and utilization by fragrant rosewood (Dalbergia odorifera) seedlings cultured with oligosaccharide addition under different lighting spectra. Forests 9:29.

McCrady RL, Comerford NB (1998). Morphological and anatomical relationships of loblolly pine fine roots. Trees – Structure & Function 12:431-437.

Riikonen J (2016). Pre-cultivation of Scots pine and Norway spruce transplant seedlings under four different light spectra did not affect their field performance. New Forests 47(4):607-619.

Riikonen J, Kettunen N, Gritsevich M, Hakala T, Särkkä L, Tahvonen R (2016). Growth and development of Norway spruce and Scots pine seedlings under different light spectra. Environmental and Experimental Botany 121:112-120.

Sun YL, Liu LD, Hong SK (2011). Eleutherococcus senticosus as a crude medicine: Review of biological and pharmacological effects. Journal of Medical Plant Research 5(25):5946-5952. DOI: 10.5897/JMPR11.728

Wan JZ, Wang CJ, Yu JH, Nie SM, Han SJ, Liu JZ, Zu YG, Wang QG (2016). Developing conservation strategies for Pinus koraiensis and Eleutherococcus senticosus by using model-based geographic distributions. Journal of Forestry Research 27(2):389-400.

Wang YJ, Wei HX, Ge LL, Sun NW, Wang T, Zhang QC, Han LB, Ge XY, Jin GY (2018). Chitosan oligosaccharide addition modifies nutrient utilization in highly-valued ornamental tree seedlings. Forestry & Environmental Science 34(3):136-144.

Wang Z, Zhao Y, Wei HX (2017). Chitosan oligosaccharide addition affects current-year shoot of post-transplant Buddhist pine (Podocarpus macrophyllus) seedlings under contrasting photoperiods. iForest – Biogeosciences & Forestry 10:715-721.

Wei HX, Xu CY, Ren J, Ma LY, Duan J, Jiang LN (2013a). Newly transplanted Larix olgensis Henry stock with greater root biomass has higher early nitrogen flux rate. Soil Science and Plant Nutrition 59(5):740-749.

Wei HX, Ren J, Zhou JH (2013b). Effect of exponential fertilization on growth and nutritional status in Buddhist pine (Podocarpus macrophyllus [Thunb.] D. Don) seedlings cultured in natural and prolonged photoperiods. Soil Science and Plant Nutrition 59(6):933-941.

Wei HX, Guo P, Zheng HF, He XY, Wang PJ, Ren ZB, Zhai C (2017). Micro-scale heterogeneity in urban forest soils affects fine root foraging by ornamental seedlings of Buddhist pine and Northeast yew. Urban Forestry & Urban Greening 28:63-72.

Wei HX, Hauer RJ, Chen GS, Chen X, He XY (2020). Growth, nutrient assimilation, and carbohydrate metabolism in Korean pine (Pinus koraiensis) seedlings in response to light spectra. Forests 11(1):44.

Zaluski D, Smolarz HD (2016). Influence of Polish climate conditions on content and the chemical variation of volatiles in the roots of six Eleutherococcus species and their potential use. Recent Natural Products 10(2):240-244.

Zhai CM, Wang M, Raman V, Rehman JU, Meng YH, Zhao JP, … Khan IA (2017). Eleutherococcus senticosus (Araliaceae) leaf morpho-anatomy, essential oil composition, and its biological activity against Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology 54(3):658-669.

Zhao J, Chen X, Wei HX, Lv J, Chen C, Liu XY, … Jia LM (2019). Nutrient uptake and utilization in Prince Rupprecht's larch (Larix principis-rupprechtii Mayr.) seedlings exposed to a combination of light-emitting diode spectra and exponential fertilization. Soil Science and Plant Nutrition 65(4):358-368.

Zhao Y, Wang Z, Wei HX, Bao YJ, Guo P (2017). Effect of prolonged photoperiod on morphology, biomass accumulation and nutrient utilization in post-transplant Taxus cuspidata seedlings. Pakistan Journal of Botany 49(4):1285-1290.

Zong HY, Liu S, Xing RG, Chen XL, Li PC (2017). Protective effect of photosynthesis and antioxidative defense system in edible rape (Brassica rapa L.) in the presence of cadmium. Ecotoxicology and Environment Safety 138:271-278.

How to Cite
GUO, S., ZHANG, S., JIA, L., XU, M., & WANG, Z. (2020). Root growth of Eleuthero (Eleutherococcus senticosus [Rupr. & Maxim.] Maxim.) seedlings cultured with chitosan oligosaccharide addition under different light spectra. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 48(2), 626-635.
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