Genetic Diversity and Population Structure Analysis of Sand Pear (Pyrus pyrifolia) ‘Nakai’ Varieties Using SSR and AFLP Markers

  • Jie CAO Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Zhongcheng ZHOU Hubei Ecology Polytechnic College, Department of Forestry Ecology, Wuhan 430070
  • Junfan TU Hubei Academy of Agricultural Sciences, Fruit and Tea Research Institute, Wuhan 430064
  • Shuiyuan CHENG Wuhan Polytechnic University, National R&D Center for Se-rich Agricultural Products Processing Technology, Wuhan 430023
  • Jinglei YAO Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Feng XU Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Guiyuan WANG Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Jian ZHANG Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Jiabao YE Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Yongling LIAO Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Weiwei ZHANG Yangtze University, College of Horticulture and Gardening, Jingzhou 434025, Hubei
  • Zexiong CHEN Chongqing University of Arts and Sciences, Research Institute for Special Plants, Chongqing 402160
Keywords: cluster analysis; genetic polymorphism; genetic structure; molecular markers; pear varieties

Abstract

In this study, the technologies of simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers were used to analyze the genetic diversity of 30 sand pear (Pyrus pyrifolia) ‘Nakai’ varieties. Ten pairs of SSR polymorphic primers were selected to amplify P. pyrifolia ‘Nakai’ varieties. A total of 90 alleles were detected. The polymorphism information content index was between 0.5578 and 0.8423, with an average of 0.7585. The selected 10 pairs of AFLP primer combinations were used to amplify the analyzed pear varieties, and 1,046 polymorphic loci were detected. The average amplification results of each primer combination detected 105 bands with an average polymorphism percentage of 86.46%. The combined data of SSR and AFLP analysis showed that the analyzed P. pyrifolia ‘Nakai’ varieties were characterized by extremely rich genetic diversity and were highly representative. According to the results of SSR, AFLP, and SSR+AFLP cluster analysis, the analyzed P. pyrifolia ‘Nakai’ varieties can be categorized into three clusters. The results of genetic structure showed that the hybridization between these P. pyrifolia ‘Nakai’ varieties resulted in the heterozygosity of genotypes. In addition, we found that ‘Nijisseik’, ‘Ejima’, and ‘Fuli’ are good parent resources among the pear varieties through observing the genetic background of the analyzed pear varieties. This study reveals the genetic diversity levels of P. pyrifolia ‘Nakai’ varieties at the molecular level, which was important in molecular identification and protection of pear germplasm resources, as well as pear variety breeding and genetic improvement.

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Published
2019-07-24
How to Cite
CAO, J., ZHOU, Z., TU, J., CHENG, S., YAO, J., XU, F., WANG, G., ZHANG, J., YE, J., LIAO, Y., ZHANG, W., & CHEN, Z. (2019). Genetic Diversity and Population Structure Analysis of Sand Pear (Pyrus pyrifolia) ‘Nakai’ Varieties Using SSR and AFLP Markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 970-979. https://doi.org/10.15835/nbha47311570
Section
Research Articles