High frequency 2n pollen formation in black poplar (Populus nigra L.) induced by colchicine
Keywords:2n pollen; colchicine; Populus nigra L.
Populus nigra L. is one of the most important male genetic donors in populus genetic improvement and tree breeding over the world. Many excellent Populus nigra clones are identified as triploids potentially obtaining by hybridization of 2n pollen and normal oogamete. This study revealed the cytological mechanism of 2n pollen formation in Populus nigra L. for the first time and concluded the best treated combination of colchicine treatment, which obtained 2n pollen with the highest rate reaching at 87.11% (even 100% 2n pollen in some floral buds) which made the polyploid hybridization utilizing 2n pollen in section Aigeiros possible.
Bastiaanssen H, Berg P, Lindhout P, Jacobsen E, Ramanna MS (2010). Postmeiotic restitution in 2n-egg formation of diploid potato. Heredity 81(1):20-27.
Blasco M, Badenes M, Naval M (2014). Colchicine-induced polyploidy in loquat (Eriobotrya japonica (Thunb.) Lindl.). Plant Cell, Tissue and Organ Culture 120(2):453-461.
Bretagnolle F, Thompson JD (2010). Tansley review no 78 - gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants. New Phytologist 129(1):1-22.
Dewitte A, Eeckhaut T, Huylenbroeck JV, Bockstaele EV (2010). Meiotic aberrations during 2n pollen formation in Begonia. Heredity 104(2):215-223.
Dewitte A, Laere KV, Huylenbroeck JV (2012). Use of 2n gametes in plant breeding. In: Abdurakhmonov IY (Ed). Plant breeding. InTech Open Access Publisher, Croatia pp 59-86.
Drazkiewicz M, Skórzyńska-Polit E, Wanke M, Swiezewska E (2003). The activity of antioxidant enzymes in Arabidopsis thaliana exposed to colchicine and H2O2. Cellular & Molecular Biology Letters 8:777-781.
Ernster L, Dallner G (1995). Biochemical, physiological and medical aspects of ubiquinone function. Biochimica et Biophysica Acta 1271(1):195-204.
Jongedijk E, Ramanna MS, Sawor Z, Hermsen JGT (1991). Formation of first division restitution (FDR) 2n-megaspores through pseudohomotypic division in ds-1 (desynapsis) mutants of diploid potato: routine production of tetraploid progeny from 2×FDR × 2×FDR crosses. Theoretical & Applied Genetics 82(5):645-656.
Jordan MA, Wilson L (1999). The use and action of drugs in analyzing mitosis. Methods in Cell Biology 61:267-295.
Kang XY (2002). Mechanism of 2n pollen occurring in Chinese white poplars. Journal of Beijing Forestry University 24(5-6):67-70.
Kang XY, Zhu ZD (1997). A study on the 2n pollen vitality and germinant characteristics of white populars. Acta Botanica Yunnanica 19(4):395-401.
Kang XY, Zhu ZD, Lin HB (1999). Study on the effective treating period for pollen chromosome doubling of Populus tomentosa × P. bolleana. Scientia Silvae Sinicae 35:21-24.
Kang XY, Zhu ZD, Zhang ZY (2000). Suitable period of high temperature treatment for 2n pollen of Populus tomentosa × P. bolleana. Journal of Beijing Forestry University 22(3):1-4.
Li Y (2001). Advances in studies of triploid poplar breeding. Bulletin of Botany 18:451-458.
Li Y, Zhu Z, Tian Y, Zhang Z, Kang X (2000). Obtaining triploids by high and low temperature treating female flower buds of white poplar. Journal of Beijing Forestry University 22(5):7-12.
Lim KB, Ramanna MS, Jong J, Jacobsen E, Tuyl J (2001). Indeterminate meiotic restitution (IMR): a novel type of meiotic nuclear restitution mechanism detected in interspecific lily hybrids by GISH. Theoretical & Applied Genetics 103(2-3):219-230.
Liu G, Li Z, Bao M (2007). Colchicine-induced chromosome doubling in Platanus acerifolia and its effect on plant morphology. Euphytica 157(1-2):145-154.
Mashkina OS, Burdaeva LM, Belozerova MM (1989). Method of obtaining diploid pollen of woody species. Lesovedenie 1:19-25.
Otto SP, Whitton J (2000). Polyploid incidence and evolution. Annual Review of Genetics 34(1):401-437.
Ramanna MS (1983). First division restitution gametes through fertile desynaptic mutants of potato. Euphytica 32(2):337-350.
Ramanna MS, Agj K, Jacobsen E (2003). Occurrence of numerically unreduced (2n) gametes in Alstroemeria interspecific hybrids and their significance for sexual polyploidisation. Euphytica 133(1):95-106.
Salmon ED, Mckeel M, Hays T (1984). Rapid rate of tubulin dissociation from microtubules in the mitotic spindle in vivo measured by blocking polymerization with colchicine. Journal of Cell Biology 99(3):1066-1075.
Taschetto, OM, Pagliarini MS (2003). Occurrence of 2n and jumbo pollen in the Brazilian ginseng (Pfaffia glomerata and P. tuberosa). Euphytica 133(1):139-145.
Tian J, Wang J, Dong L, Dai F, Wang J (2015). Pollen variation as a response to hybridisation in Populus L. section Aigeiros Duby. Euphytica 206(2):1-11.
Wanke M, Dallner G, Swiezewska E (2000). Subcellular localization of plastoquinone and ubiquinone synthesis in spinach cells. BBA-Biomembranes 1463(1):188-194.
Xi XJ, Jiang XB, Li D, Guo LQ, Zhang JF, Wei ZZ, Li BL (2011). Induction of 2n pollen by colchicine in Populus × popularis and its triploids breeding. Silvae Genetica 60(1-6):155-160.
Xu WT, Zhao J, Zhao N, Guo LQ, Zhang JG, Xia C, Zhang JF (2011). Meiosis of pollen mother cells and their asynchrony in Populus nigra L. Acta Botanica Boreali-Occidentalia 31(10):1981-1987.
Zhang JF (2006). Mechanisms of 2n pollen formation and its inducing technique of poplar in section Aigeiros. Beijing: Beijing Forestry University.
Zhang S, Qi L, Chen C, Li X, Song W, Chen R, Han S (2004). A report of triploid Populus of the section Aigeiros. Silvae Genetica 53(1-6):69-75.
Zhang ZY, Kang XY (2010). Cytological characteristics of numerically unreduced pollen production in Populus tomentosa Carr. Euphytica 173(2):151-159.
Zhou S, Ramanna M, Visser R, Tuyl J (2008). Analysis of the meiosis in the F1 hybrids of Longiflorum × Asiatic (LA) of lilies (Lilium) using genomic in situ hybridization. Journal of Genetics and Genomics 35(11):687-695.
Zhu ZD, Kang XY, Zhang ZY (1998). Studies on selection of natural triploids of Populus tomentosa. Scientia Silvae Sinicae 34(4):22-31.
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