High frequency 2n pollen formation in black poplar (Populus nigra L.) induced by colchicine

Wenting XU; Yan ZHANG; Liqin GUO; Jian ZHAO; Zunzheng WEI; Jinfeng ZHANG

doi:10.15835/nbha47311521

Authors

Wenting XU Beijing Forestry University, College of Biological Sciences and Technology, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants, Biological Engineering of State Forestry Administration, Beijing, 100083 (CN)
Yan ZHANG Beijing Forestry University, College of Biological Sciences and Technology, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants, Biological Engineering of State Forestry Administration, Beijing, 100083 (CN)
Liqin GUO Beijing Forestry University, College of Biological Sciences and Technology, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants, Biological Engineering of State Forestry Administration, Beijing, 100083 (CN)
Jian ZHAO Beijing Forestry University, College of Biological Sciences and Technology, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants, Biological Engineering of State Forestry Administration, Beijing, 100083 (CN)
Zunzheng WEI Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing Vegetable Research Center, Beijing, 100091 (CN)
Jinfeng ZHANG Beijing Forestry University, College of Biological Sciences and Technology, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants, Biological Engineering of State Forestry Administration, Beijing, 100083 (CN)

DOI:

https://doi.org/10.15835/nbha47311521

Keywords:

2n pollen; colchicine; Populus nigra L.

Abstract

Populus nigra L. is one of the most important male genetic donors in populus genetic improvement and tree breeding over the world. Many excellent Populus nigra clones are identified as triploids potentially obtaining by hybridization of 2n pollen and normal oogamete. This study revealed the cytological mechanism of 2n pollen formation in Populus nigra L. for the first time and concluded the best treated combination of colchicine treatment, which obtained 2n pollen with the highest rate reaching at 87.11% (even 100% 2n pollen in some floral buds) which made the polyploid hybridization utilizing 2n pollen in section Aigeiros possible.

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