Biohardening of Arabidopsis thaliana Seeds and Seedlings with Fraser Photinia Associated Bacterium (PGB_invit) in In vitro Conditions

Maria BATOOL; Özlem AKKAYA; Mine GÜL ŞEKER; Yelda ÖZDEN ÇİFTÇİ

doi:10.15835/nbha47311488

Authors

Maria BATOOL Gebze Technical University, Faculty of Basic Sciences, Department of Molecular Biology and Genetics, 41400, Kocaeli (TR)
Özlem AKKAYA Gebze Technical University, Faculty of Basic Sciences, Department of Molecular Biology and Genetics, 41400, Kocaeli (TR)
Mine GÜL ŞEKER Gebze Technical University, Faculty of Basic Sciences, Department of Molecular Biology and Genetics, 41400, Kocaeli (TR)
Yelda ÖZDEN ÇİFTÇİ Gebze Technical University, Faculty of Basic Sciences, Department of Molecular Biology and Genetics, 41400, Kocaeli (TR)

DOI:

https://doi.org/10.15835/nbha47311488

Keywords:

Arabidopsis thaliana, endophytic bacteria, plant-bacteria interactions, virD1 gene

Abstract

The aim of this study was to analyze possible positive effects of putatively endophytic PGPB (PGB_invit), which was isolated from long-term in vitro cultured fraser photinia microshoots, on seed and 7-day old seedling stages of Arabidopsis thaliana. Seeds and in vitro-germinated seedlings were inoculated with 10⁷ CFU/mL and 10⁸ CFU/mL active (A) and inactive (I) endophytic bacterial populations along with their mix compositions (A+I) and suspended in MPYE broth together with their controls (untreated ones). 14 days old seedlings were evaluated for various plant growth parameters [i.e., shoot and root fresh weight (FW), shoot length (SL), shoot and root dry weight (DW), root length (RL) and photosynthetic pigments including chlorophyll a, b and carotenoids of plantlets] as well as endophytic and rhizospheric trait of bacteria. Positive effects of inactive and active bacterium on FW, DW and photosynthetic pigments for 7-day inoculated seedlings were recorded whereas an increase in photosynthetic contents for seed stage inoculations was observed. Rhizospheric and endophytic colonization of the bacteria was confirmed by PCR with the presence of virD1 gene, which is previously recorded to be existed in the plasmid of bacterium after inoculation. Overall, these results demonstrated that this peculiar putative endophytic bacterium being beneficial in active and even more useful in inactive form for A. thaliana when optimum conditions and concentrations are used. Moreover, presence of virD1 gene suggested its potential possibility to be used in bioengineering along with various other beneficial PGPR features as biofertilizer.

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