Phenolic Profiles, Antimicrobial and Cytotoxic Properties of Both Micropropagated and Naturally Growing Plantlets of Calamintha sylvatica subsp. sylvatica Bromf.

Mustafa CÜCE; Tuba BEKİRCAN; Abdul Hafeez LAGHARI; Münevver SÖKMEN; Atalay SÖKMEN; Evren ÖNAY UÇAR; Ali Osman KILIÇ

doi:10.15835/nbha47411474

Authors

Mustafa CÜCE Giresun University, Şebinkarahisar School of Applied Sciences, Department of Food Technology, 28400, Giresun (TR)
Tuba BEKİRCAN Eurasia University, Faculty of Science and Art, Department of Biochemistry, 61080, Trabzon (TR)
Abdul Hafeez LAGHARI Karadeniz Technical University, Faculty of Science, Department of Chemistry, 61080, Trabzon (TR)
Münevver SÖKMEN Karadeniz Technical University, Faculty of Science, Department of Chemistry, 61080, Trabzon; King Saud University, College of Science, Department of Zoology, 11451, Riyadh (SA)
Atalay SÖKMEN Konya Food and Agriculture University, Faculty of Agriculture and Natural Sciences, Department of Plant Production & Technologies, 42080, Konya; King Saud University, College of Science, Department of Zoology, 11451, Riyadh (SA)
Evren ÖNAY UÇAR İstanbul University, Faculty of Science, Department of Molecular Biology and Genetic, 34134 İstanbul (TR)
Ali Osman KILIÇ Karadeniz Technical University, Faculty of Medicine, Department of Medical Microbiology, 61080 Trabzon (TR)

DOI:

https://doi.org/10.15835/nbha47411474

Keywords:

antimicrobial activity; Calamintha sylvatica; cytotoxic activity; micropropagation; rosmarinic acid

Abstract

A rapid micropropagation protocol was designed to produce Calamintha sylvatica plantlets by using nodal segments as explants for the shoot formation. 6-BA favored the highest shoot formation and biomass yield, whilst kinetin was found superior for the highest shoot length (38.97 ± 2.85 mm) and node numbers (2.89 ± 0.63). Rosmarinic acid was detected as major phenolic acid, ranging from 7.59 mg/100 g to 81.44 mg/100 g. Hexane extracts from natural and in vitro propagated plantlets showed activity only against Staphylococcus aureus ATCC 25923 with MIC values at 6.25 and 3.33 m/mL, respectively while in the latter case, extracts from natural plantlets exerted higher cytotoxic activity than those of micropropagated ones (IC₅₀ values were 83 µg/mL and 98 µg/mL on HeLa cells, respectively). C. sylvatica showed high micropropagation performance and produced remarkable amount of rosmarinic acid in vitro as well as antimicrobial and cytotoxic effect.

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