Phytochemical and Antioxidant Properties of Fresh Fruits and Some Traditional Products of Wild Grown Raspberry (Rubus idaeus L.)

Bojana VELJKOVIĆ; Violeta JAKOVLJEVIĆ; Milan STANKOVIĆ; Zora DAJIĆ-STEVANOVIĆ

doi:10.15835/nbha47311465

Authors

Bojana VELJKOVIĆ State University of Novi Pazar, Department of Biomedical Sciences, Vuka Karadžića bb, 36300 Novi Pazar (RS)
Violeta JAKOVLJEVIĆ State University of Novi Pazar, Department of Biomedical Sciences, Vuka Karadžića bb, 36300 Novi Pazar (RS)
Milan STANKOVIĆ University of Kragujevac, Faculty of Sciences, Institute for Biology and Ecology, Radoja Domanovića 12, 34000 Kragujevac (RS) https://orcid.org/0000-0001-9861-7700
Zora DAJIĆ-STEVANOVIĆ University of Belgrade, Department of Agrobotany, Nemanjina 6, 11080 Zemun-Belgrade (RS) https://orcid.org/0000-0001-9275-4499

DOI:

https://doi.org/10.15835/nbha47311465

Keywords:

antioxidant activity; fruit; juice; phytochemical; sweet preserve

Abstract

The current study investigated and compared phytochemical and antioxidant activity of fresh fruit and some traditional products of Rubus idaeus grown in mountain region of Serbia. The total organic acid, total sugar content, total phenolics, flavonoids, tannins, anthocyanins and vitamin C were evaluated. The antioxidant activities were evaluated using two antioxidant systems 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The fresh fruit contained highest amount of vitamin C (46.62 mg AA g^-1) and total organic acids (882.22 mg CA g^-1). The sweet preserve had highest content of total phenolics (200.83 mg GA g^-1), flavonoids (12.85 mg RU g^-1) and tannins (39.11 mg g^-1). The juice had the highest total anthocyanin content (107.22 µg mL^-1) and total sugar content (25 °Brix). The best antioxidant activity in ABTS assay had juice (IC₅₀= 4.87 µmol TE g^-1), followed by sweet preserve (IC₅₀= 5.14 µmol TE g^-1), almost identical to standard gallic acid. In the DPPH free radical scavenging assay, sweet fruit preserve showed significant better antioxidant activity (IC₅₀ = 41.27 µg mL^-1) compared to juice (IC₅₀ = 106.07 µg mL^-1) and fresh fruit (IC₅₀ = 294.79 µg mL^-1). Our results indicated promising perspectives for usage of R. idaeus fresh fruits and traditional products studied with considerable levels of vitamin C, bioactive compounds and antioxidant activity.

References

Alice LA, Campbell CS (1999). Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. American Journal of Botany 86(1):81-97.

Amakura Y, Umino Y, Tsuji S, Tonogai Y (2001). Influence of jam processing on the radical scavenging activity and phenolic content in berries. Journal of Agricultural and Food Chemistry 48(12):6292-6297.

Bartolomé AP, Rupérez P, Fúster C (1995). Pineapple fruit: morphological characteristics, chemical composition and sensory analysis of Red Spanish and Smooth Cayenne cultivars. Food Chemistry 53(1):75-79.

Bobinaitė R, Viškelis P, Venskutonis PR (2012). Variation of total phenolics, anthocyanins, ellagic acid and radical scavenging capacity in various raspberry (Rubus spp.) cultivars. Food Chemistry 132:1495-1501.

Brighente IMC, Dias M, Verdi LG, Pizzolatti MG (2007). Antioxidant activity and total phenolic content of some Brazilian species. Pharmaceutical Biology 45(2):156-161.

Çekiç C, Özgen M (2010). Comparison of antioxidant capacity and phytochemical properties of wild and cultivated red raspberries (Rubus idaeus L.). Journal of Food Composition and Analysis 23(6):540-544.

Chen L, Xin X, Zhang H, Yuan Q (2013). Phytochemical properties and antioxidant capacities of commercial raspberry varieties. Journal of Functional Foods 5(1):508-515.

Gasperotti M, Masuero D, Vrhovsek U, Guella G, Mattivi F (2010). Profiling and accurate quantification of Rubus ellagitannins and ellagic acid conjugates using direct UPLC-Q-TOF HDMS and HPLC-DAD analysis. Journal of Agricultural and Food Chemistry 58(8):4602-4616.

Gülçin I, Topal F, Çakmakçi R, Bilsel M, Gören AC, Erdogan U (2011). Pomological features, nutritional quality, polyphenol content analysis, and antioxidant properties of domesticated and 3 wild ecotype forms of raspberries (Rubus idaeus L.). Journal of Food Science 76(4):585-593.

Hassani S, Shariatpanahi M, Tavakoli F, Nili-Ahmadabadi A, Abdollahi M (2015). The changes of bioactive ingredients and antioxidant properties in various berries during jam processing. International Journal of Biosciences 6:172-179.

Horwitz W (1975). Official methods of analysis (Vol. 222). Washington, DC: Association of Official Analytical Chemists.

Hosu A, Cristea VM, Cimpoiu C (2014). Analysis of total phenolic, flavonoids, anthocyanins and tannins content in Romanian red wines: Prediction of antioxidant activities and classification of wines using artificial neural networks. Food Chemistry 150:113-118.

Hussein HA, Ewadh MJ, AL Gazally ME (2017). Detection and estimation of tannic acid in Rubus idaeus (red raspberry) as natural chelating agent for iron using HPLC. Research Journal of Pharmaceutical Biological and Chemical Sciences 8(2):345-351.

Jakovljević VD, Milićević JM, Ðelić GT, Vrvić MM (2016). Antioxidant activity of Ruscus species from Serbia: Potential new sources of natural antioxidants. Hemijska Industrija 70(1):99-106.

Kadakal Ç, Duman T, Ekinci R (2017). Thermal degradation kinetics of ascorbic acid, thiamine and riboflavin in rosehip (Rosa canina L) nectar. Food Science and Technology 38(4), http://dx.doi.org/10.1590/1678-457x.11417.

Karakashova L, Stamatovska V, Babanovska-Milenkovska F (2012). The quality properties of raspberry jams with different sweeteners. In: International Symposium for Agriculture and Food, XXXVII Faculty-Economy Meeting, IV Macedonian Symposium for Viticulture and Wine Production, VII Symposium for Vegetables and Flower Production, Skopje, Macedonia, 12-14 December 2012, pp 848-855.

Kuskoski EM, Asuero GA, Troncoso AM, Mancini-Filho J, Fett R (2005). Aplicación de diversos métodos químicos para determinar actividad antioxidante em pulpa de frutos. Food Science and Technology 25(4):726-732.

Kim DO, Padilla-Zakour OI (2004). Jam processing effect on phenolics and antioxidant capacity in anthocyanin-rich fruits: cherry, plum, and raspberry. Journal of Food Science 69(9):S395-S400.

Koponen JM, Happonen AM, Mattila PH, Törrönen AR (2007). Contents of anthocyanins and ellagitannins in selected foods consumed in Finland. Journal of Agricultural and Food Chemistry 55(4):1612-1619.

Kostecka-Gugała A, Ledwożyw-Smoleń I, Augustynowicz J, Wyżgolik G, Kruczek M, Kaszycki P (2015). Antioxidant properties of fruits of raspberry and blackberry grown in central Europe. Open Chemistry 13(1):1313-1325.

Krauze-Baranowska M, Majdan M, Hałasa R, Głód D, Kula M, Fecka I, Orzeł A (2014). The antimicrobial activity of fruits from some cultivar varieties of Rubus idaeus and Rubus occidentalis. Food & Function 5(10):2536-2541.

Le Bourvellec C, Gouble B, Bureau S, Reling P, Bott R, Ribas-Agusti A, Audergon J-M, Renard CMGC (2018). Impact of canning and storage on apricot carotenoids and polyphenols. Food Chemistry 240:615-625.

Mapson LW (1970). Biosynthesis of ethylene and the ripening of fruit. Endeavour 29:29-33.

Mazur SP, Nes A, Wold AB, Remberg SF, Aaby K (2014). Quality and chemical composition of ten red raspberry (Rubus idaeus L.) genotypes during three harvest seasons. Food Chemistry 160:233-240.

Mikulic‐Petkovsek M, Schmitzer V, Slatnar A, Stampar F, Veberic R (2012). Composition of sugars, organic acids, and total phenolics in 25 wild or cultivated berry species. Journal of Food Science 77(10):1064-1070.

Milivojevic J, Maksimovic V, Nikolic M, Bogdanovic J, Maletic R, Milatovic D (2011). Chemical and antioxidant properties of cultivated and wild Fragaria and Rubus berries. Journal of Food Quality 34(1):1-9.

Mullen W, Mcginn J, Lean MEJ, Maclean MR, Gardner P, … Crozier A (2002). Ellagitannins, flavonoids, and other phenolics in red raspberries and their contribution to antioxidant capacity and vasorelaxation properties. Journal of Agricultural and Food Chemistry 50(18):5191-5196.

Pantelidis GE, Vasilakakis M, Manganaris GA, Diamantidis GR (2007). Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, blackberries, red currants, gooseberries and Cornelian cherries. Food Chemistry 102(3):777-783.

Poyrazoğlu E, Gökmen V, Artik N (2002). Organic acids and phenolics compouns in pomegrantates (Punica granatum L.) grown in Turkey. Journal of Food Composition and Analysis 15(5):567-575.

Pritts MP (2003). Raspberries and related fruits. In: Encyclopedia of Food Sciences and Nutrition (Second Edition) 4916-4921.

Rauha JP, Remes S, Heinonen M, Hopia A, Kähkönen M,… Vuorela P (2000). Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. International Journal of Food Microbiology 56(1):3-12.

Rickman JC, Barrett DM, Bruhn CM (2007). Nutritional comparison of fresh, frozen and canned fruits and vegetables. Part 1. Vitamins C and B and phenolic compounds. Journal of the Science of Food and Agriculture 87(6):930-944.

Sablani SS, Andrews PK, Davies NM, Walters T, Saez H, … Mohekar PR (2010). Effect of thermal treatments on phytochemicals in conventionally and organically grown berries. Journal of the Science of Food and Agriculture 90(5):769-778.

Sariburun E, Sahin S, Demir C, Turkben C, Uylaser V (2010). Phenolic content and antioxidant activity of raspberry and blackberry cultivars. Journal of Food Science 75(4):328-335.

Serrano M, Díaz-Mula HM, Valero D (2011). Antioxidant compounds in fruits and vegetables and changes during postharvest storage and processing. Stewart Postharvest Review 7(1):1-10.

Siriwoharn T, Wrolstad RE, Finn CE, Pereira CB (2004). Influence of cultivar, maturity, and sampling on blackberry (Rubus L. hybrids) anthocyanins, polyphenolics, and antioxidant properties. Journal of Agricultural and Food Chemistry 52(26):8021-8030.

Skrovankova S, Sumczynski D, Mlcek J, Jurikova T, Sochor J (2015). Bioactive compounds and antioxidant activity in different types of berries. International Journal of Molecular Sciences 16(10):24673-24706.

Stevens R, Buret M, Duffé P, Garchery C, Baldet P, … Causse M (2007). Candidate genes and quantitative trait loci affecting fruit ascorbic acid content in three tomato populations. Plant Physiology 143(4):1943-1953.

Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K (1994). A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Bioscience, Biotechnology and Biochemistry 58(10):1780-1783.

Tamer CE (2012). A Research on raspberry and blackberry marmalades produced from different cultivars. Journal of Food Processing and Preservation 36(1):74-80.

Van Boekel M, Pellegrini N, Fogliano V, Eisenbrand G (2010). A review on the beneficial aspects of food processing. Molecular Nutrition & Food Research 54(9):1215-1247.

Veda S, Kamath A, Platel K, Begum K, Srinivasan K (2006). Determination of bioaccessibility of β‐carotene in vegetables by in vitro methods. Molecular Nutrition & Food Research 50(11):1047-1052.

Venskutonis PR, Dvaranauskaite A, Labokas J (2007). Radical scavenging activity and composition of raspberry (Rubus idaeus) leaves from different locations in Lithuania. Fitoterapia 78(2):162-165.

Wada L, Ou B (2002). Antioxidant activity and phenolic content of Oregon cranberries. Journal of Agricultural and Food Chemistry 50(12):3495-3500.

Wang SY, Lin HS (2000). Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry 48(2):140-146.

Wang SY, Chen CT, Wang CY (2009). The influence of light and maturity on fruit quality and flavonoid content of red raspberries. Food Chemistry 112:676-684.

Zadernowski R, Naczk M, Nesterowicz J (2005). Phenolic acid profiles in some small berries. Journal of Agricultural and Food Chemistry 53(6):2118-2124.

Wootton-Beard PC, Moran A, Ryan L (2011). Stability of the total antioxidant capacity and total polyphenol content of 23 commercially available vegetable juices before and after in vitro digestion measured by FRAP, DPPH, ABTS and Folin–Ciocalteu methods. Food Research International 44(1):217-224.

Zafrilla P, Ferreres F, Tomás-Barberán FA (2001). Effect of processing and storage on the antioxidant ellagic acid derivatives and flavonoids of red raspberry (Rubus idaeus) jams. Journal of Agricultural and Food Chemistry 49(8):3651-3655.

Zhang Y, Zhang Z, Yang Y, Zu X, Guan DI, Guan Y (2011). Diuretic activity of Rubus idaeus L (Rosaceae) in rats. Tropic Journal of Pharmaceutical Research 10(3):243-248.

Zorenc Z, Veberic R, Koron D, Mikulic-Petkovsek M (2017). Impact of raspberry (Rubus idaeus L.) primocane tipping on fruit yield and quality. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 45(2):417-424.

Tatić B (1972). Flora SR Srbije. SANU, Belgrade, Serbia.