Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis

  • Xiao Dong CAI Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • Jin Jin CHENG Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
  • Yuan Shan ZHANG Yangtze University, College of Horticulture and Gardening, Jingzhou, Hubei 434025
Keywords: in vitro; morphological characterization; plant growth regulator; rapid propagation; Trichosanthes kirilowii


Trichosanthes kirilowii Maxim is a perennial plant possessing great medicinal and edible value. In this study, an efficient rapid propagation system was developed for in vitro production of high-quality plantlets of T. kirilowii cv. ‘Yuelou-2’ via organogenesis. Shoots were established from the nodal stems of this plant by cultured on Murashige and Skoog (MS) basal medium containing different concentrations of naphthalene acetic acid (NAA), indole-3-butyric acid (IBA) and 6-benzyladenine (6-BA) according to a L9 (34) orthogonal array design. MS medium supplemented with 0.5 mg l-1 6-BA alone conferred significant enhancement of shoot induction and development by variance and statistical analysis of shoot induction frequency, shoot length, as well as lateral shoot number and total node number per explant. Rooting percentage, root morphological characteristics, plantlet survival rate, as well as plantlet growth performance in vitro and ex vitro were comprehensively assessed, and results showed that the addition of 1.0 mg l-1 NAA to 1/2 MS medium was the most responsive for root induction and production of high-quality plantlets from the regenerated shoots. The in vitro propagation system described here has the potential for large-scale and rapid production of in vitro plantlets of seed variety of T. kirilowii in a sustainable manner.



In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.



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How to Cite
CAI, X. D., CHENG, J. J., & ZHANG, Y. S. (2019). Optimization of a Rapid Propagation System for Mass Production of High-Quality Plantlets of Trichosanthes kirilowii cv. ‘Yuelou-2’ via Organogenesis. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3). https://doi.org/10.15835/nbha47311450
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