Physiological Effects of the Fungicide Azoxystrobin on Wheat Seedlings under Extreme Heat
Keywords:antioxidant activity; azoxystrobin; chlorophyll fluorescence; heat stress; wheat
Azoxystrobin (AZ) is not only a fungicide used for disease control, but also a protective chemical for crops against specific stresses. The physiological mechanism of the fungicide AZ in protecting against heat (HT, 46 °C) stress in wheat (Triticum aestivum L.) seedlings was investigated. ‘Taichung SEL-2’ variety seedlings were pretreated with 0.4, 4, 40, 80, and 120 mg L-1 of AZ for 4 d. Next AZ-pretreated and untreated seedlings were subjected to HT for 1 h followed by 1000 μmol m-2 s-1 lighting for 20 min. HT induced oxidant stress which resulted in a decrease in the reducing power, an increase in malondialdehyde, and enhanced enzyme activities of ascorbate peroxidase (APX) and catalase (CAT) in leaves of untreated seedlings. However, AZ-pretreated seedlings under HT displayed reductions in chlorophyll fluorescence, APX and CAT activities, and the 1,1-diphenyl-2-picryl-hydrazyl scavenging capacity. Physiological damage caused by HT was aggravated by an increase in the AZ concentration. In addition, increased photosynthetic pigments were also observed in leaves of AZ-pretreated and HT-exposed seedlings. The results suggest that AZ does not provide a protective effect against HT stress.
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