The Propagation of Rosa gallica ‘Tuscany Superb’ by Root Cuttings with the Use of IBA and Biostimulants


  • Marta Joanna MONDER Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973 Warsaw (PL)
  • Konrad WOLIŃSKI Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973 Warsaw (PL)
  • Maciej NIEDZIELSKI Polish Academy of Sciences Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973 Warsaw (PL)



carbohydrates; old roses; rhizogenesis; rhizomes; suckers; titanium


The research was aimed at studying the possibility of an alternative method of propagation the difficult-to-root Rosa gallica ‘Tuscany Superb’. The root cuttings of three thickness were rooted using IBA preparations (Chryzotop green, Rhizopon AA 020XX) and biostimulants (Tytanit, Root JuiceTM). The shoots disappeared before the growth of the roots. At the first days of growth, the both shoots and roots elongated upright without polarity. The primordia of the first type of root/shoot buds were situated in cambium zone as the subtending traces of near the rays visible on the cross sections. Moreover, the second types of buds were located in pericycle tissues. The swellings suggested inherence buds were disappearing before rooting. The content of reducing and non-reducing carbohydrates was lower at the time of harvesting the rooted cuttings than before rooting. The thickness of the root cuttings had a significant importance for growth parameters and content of carbohydrates in rooted cuttings. The most useful root cuttings, those achieving the highest mean rooting percentage are thick (76.8%) and medium (67.9%) size. Preparations or biostimulants had a differential influence on rooting process and none of them unequivocally guaranteed an improvement in the rooting percentage of cuttings, parameters of growth or content of carbohydrates. The Rhizopon AA 020XX (90.0%) in thick and Chryzotop green (87.5%) in medium cuttings increased the rooting percentage significantly. Root JuiceTM (0.1%, 0.2%) and Tytanit (0.04%) were also enough positive activity in medium cuttings and can replace IBA preparations.


Metrics Loading ...


BioBizz Worldwide BV (2017). Root JuiceTM. Retrieved 2017 February 12 from

Bosela MJ, Ewers FW (1997). The mode of origin of root buds and root sprouts in the clonal tree Sassafras albidum (Lauraceae). American Journal of Botany 84(11):1466-1481.

Calvo P, Nelson L, Kloepper JW (2014). Agricultural uses of plant biostimulants. Plant Soil 383(1-2):3-41.

Denaxa N-K, Vemmos SN, Roussos PA (2012). The role of endogenous carbohydrates and seasonal variation in rooting ability of cuttings of an easy and a hard to root olive cultivars (Olea europaea L.). Scientia Horticulture 143:19-28.

Dumon JC, Ernst WHO (1988). Titanium in plants. Journal of Plant Physiology 133(2):203-209.

Ercişli S, Eşitken A, Anapali O, Şahin U (2005). Effects of substrate and IBA-concentration on adventitious root formation on hardwood cuttings of Rosa dumalis. Acta Horticulturae 751:149-152.

Ermel FF, Vosozo S, Charpentier JP, Jay-Allemand C, Catesson AM, Couée I (2000). Mechanism of primordium formation during adventitious development from walnut cotyledon explants. Planta 211(4):563-574.

Hartmann HT, Kester DE, Davies FT, Geneve RL (2011). Plant propagation: Principles and practices. 7 th Ed Pearson Education, Upper Saddle River, NJ, USA.

Hoşafçi H, Arslan N, Sarihan EO (2005). Propagation of dogrose (Rosa canina L) plants by softwood cuttings. Acta Horticulturae 690:139-142.

Husen A (2008). Stock-plant etiolation causes drifts in total soluble sugars and anthraquinones, and promotes adventitious root formation in teak (Tectona grandis L.) coppice shoots. Plant Growth Regulation 54:13-21.

Kazankaya A, Yörük E, Doğan A (2005). Effect of IBA on rooting of Rosa canina hardwood cuttings from Lake Van region, Turkey. Acta Horticulturae 690:153-158.

Kuzel S, Cigler P, Hruby P, Vydra M, Pavlikova JD, Tlustos P (2007). The effect of simultaneous magnesium application on the biological effects of titanium. Plant, Soil and Environment 53(1):16-23.

Ky-Dembele C, Tigabu M, Bayala J, Savadogo P, Boussim IJ, Odén C (2010). Clonal propagation of Detarium microcarpum from root cuttings. Silva Fennica 44(5):775-785.

Leskó K, Stefanovits-Bányai É, Pais I, Simon-Sarkadi L (2002). Effect of cadmium and titanium - ascorbate stress on biological active compounds in wheat seed lings. Journal of Plant Nutrition 25(11):2571-2581.

Lyu S, Wei X, Chen J, Wang C, Wang X, Pan D (2017). Titanium as a beneficial element for crop production. Frontiers in Plant Sciences 8:597.

Mahmoud EA (1964). Factors affecting the rooting of hardwood cuttings of R multiflora. Master of Science Thesis. Department of Horticulture. The University of Arizona, USA.

Marcinek B, Hetman J (2008). The effect of foliage feeding on the structure of yield, dry weight content and macroelements in the corms of Sparaxis tricolor Ker-Gawl. Acta Scientiarum Polonorum, Hortorum Cultus 7(4):89-99.

Monder MJ, Niedzielski M, Woliński K (2014). Effect of rooting preparations on protein, chlorophyll and carotenoid content in leaves of Rosa gallica ‘Duchesse d’Angoulême’ cuttings. Dendrobiology 72:29-40.

Monder MJ (2016). Study on propagation of shrub and pillar roses by hardwood cuttings with auxins preparations. Acta Scientiarum Polonorum Hortorum Cultus 11(2):93-103.

Monder MJ, Pacholczak A (2018). Preparations of plant origin enhance carbohydrate content in plant tissues of rooted cuttings of rambler roses Rosa beggeriana ‘Polstjärnan’ and Rosa helenae ‘Semiplena’. Acta Agriculturae Scandinavica, Section B - Soil & Plant Science 68(3):189-198.

Nasri F, Fadakar A, Saba MK, Yousefi B (2015). Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.). Journal of Agricultural Sciences 60(3):263-275.

Perala DA (1978). Aspen suckers production and growth from outplanted root cuttings. North Central Experiment Forest Station Research Note, Forest Service USDA: NC-241, pp 1-4.

Schier GA, Zasada JC (1973). Role of the carbohydrate reserves in the development of root suckers in Populus tremuloides. Canadian Journal of Forest Research 3(2):243-250.

Snedden J, Landhäusser SM, Lieffers VJ, Charleson LR (2010). Propagating trembling aspen from root cuttings: impact of storage length and phenological period of root donor plants. New Forest 39(2):169-182.

Terpiński Z. (1984). Szkółkarstwo ozdobne. [The ornamental plants nursery]. Warsaw (PL): Państwowe Wydawnictwo Rolnicze i Leśne.

Toczko M, Grzelińska A (2001). Materiały do ćwiczeń z biochemii. Warsaw. Wydawnictwo SGGW.




How to Cite

MONDER, M. J., WOLIŃSKI, K., & NIEDZIELSKI, M. (2019). The Propagation of Rosa gallica ‘Tuscany Superb’ by Root Cuttings with the Use of IBA and Biostimulants. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 691–698.



Research Articles
DOI: 10.15835/nbha47311436

Most read articles by the same author(s)