Cross Compatibility Analysis to Identify Suitable Parents of Tagetes erecta and T. patula for Heterotic Hybrid Breeding

  • Huali ZHANG Beijing Institute of Landscape and Architecture, Beijing Key Lab of Green Plant Breeding, No.7 Huajiadi, Chaoyang District, Beijing
  • Haibo XIN Beijing Institute of Landscape and Architecture, Beijing Key Lab of Green Plant Breeding, No.7 Huajiadi, Chaoyang District, Beijing
  • Richen CONG Beijing Institute of Landscape and Architecture, Beijing Key Lab of Green Plant Breeding, No.7 Huajiadi, Chaoyang District, Beijing
  • Zijing LI Beijing Institute of Landscape and Architecture, Beijing Key Lab of Green Plant Breeding, No.7 Huajiadi, Chaoyang District, Beijing
  • Lina SONG Beijing Institute of Landscape and Architecture, Beijing Key Lab of Green Plant Breeding, No.7 Huajiadi, Chaoyang District, Beijing
  • Zunzheng WEI Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Beijing Engineering Technology Research Center of Functional Floriculture, Zhanghua Road No. 50, Haidian District, Beijing
Keywords: heterosis; interspecific hybridization; Tagetes erecta; T. patula

Abstract

Seven interspecific crosses of Tagetes erecta × T. patula were conducted in this study. The cross compatibility index, seed setting rate, germination rate, phenotype, ploidy level, heterosis over male parent and field performance were tested. The results showed that different cross compatibility indices of Tagetes vary greatly, from 10.44~114.41, which is far less than that of the intraspecific hybridization S-121 × I-506 index. The seed setting rate of each cross ranged from 15.67% to 49.0%, and the cross S-121 × TP-512 scored the highest seed setting rate, which was higher than that of the intraspecific cross S-121 × I-506 (35.85%). Compared with the male parent, F1 has higher plant height, wider crown width, larger flower diameter and more ray florets and earlier bloom with two exceptions. The S-121 of T. erecta was diploid. All of the male parents of T. patula were tetraploids, and the combinations were triploids. The traits of plant height, plant width, flower size and number of ray florets showed positive heterosis over the male parents, and the days leading to flowering showed both positive and negative ones. Finally, S-121 × TP-379 and S-121 × TP-512 were selected as the two best combinations. This study confirmed that the two species of marigold could be crossed for better F1 varieties with improved performance.

Metrics

Metrics Loading ...

References

Ai Y, He YH, Hu Y, Zhang Q, Pan C, Bao MZ (2014). Characterization of a novel male sterile mutant of Tagetes patula induced by heat shock. Euphytica 200(2):159-173.

Ai Y, Zhang CL, Sun YL, Wang WN, He YH, Bao MZ (2017). Characterization and functional analysis of five MADS-box B class genes related to floral organ identification in Tagetes erecta. PLoS One 12(1):e0169777.

Ai Y, Zhang QH, Pan C, Zhang HY, Ma S, He YH, Bao MZ (2015). A study of heterosis, combining ability and heritability between two male sterile lines and ten inbred lines of Tagetes patula. Euphytica 203(2):349-366.

Baciu A, Pamfil D, Sestras A, Mihalte L, Sestras R (2012). Heterosis studies for response to Aphis fabae attack in Calendula. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture 69(1):40-47.

Chen JF, Lin YJ (1982). Chromosome pairing in interspecific hybrids of Tagetes patula and Tagetes erecta marigold. Cytologia 47(3-4):737-742.

Cicevan R, Al Hassan M, Sestras AF, Prohens J, Vicente O, Sestras RE, Boscaiu M (2016). Screening for drought tolerance in cultivars of the ornamental genus Tagetes (Asteraceae). PeerJ 4:e2133.

Deng YM, Teng NJ, Chen SM, Chen FD, Guan ZY, Song AP, Chang QS (2010). Reproductive barriers in the intergeneric hybridization between Chrysanthemum grandiflorum (Ramat) Kitam and Ajania przewalskii Poljak. (Asteraceae). Euphytica 174(1):41-50.

Deng YM, Ye XQ (2012). The prefertilization reproductive barriers and overcoming methods of horticultural crops distant hybridization. Acta Agriculturae Boreali-Sinica 27(S1):81-86.

He YH, Ning GG, Sun YL, Qi YC, Bao MZ (2009). Identification of a SCAR marker linked to a recessive male sterile gene (Tems) and its application in breeding of marigold (Tagetes erecta). Plant Breeding 128(1):92-96.

He YH, Sun YL, Zheng RR, Ai Y, Cao Z, Bao MZ (2016). Induction of tetraploid male sterile Tagetes erecta by colchicine treatment and its application for interspecific hybridization. Horticultural Plant Journal 2(5):284-292.

Li FR, Zhang JC, Xu JR, Zhou JH (2005). Studies on the cross-breeding of Tagetes erecta L. × Tagetes patula L. and the sterility of hybrid. Inner Mongolia Agricultural University 26(2):51-54.

Li XL, Chen FD, Zhao HB (2008). Compatibility of interspecific cross in Dendranthema genus. Acta Horticulturae Sinica 35(2):257-262.

Namita N, Singh KP, Bharadwaj C, Sharma TR, Sonah H, Raju DVS, Deshmukh RK (2011). Gene action and combining ability analysis for flower yield and its component traits in interspecific hybrids of marigold (Tagetes spp). Indian Journal of Agricultural Sciences 81(9):807-811.

Namita N, Singh KP, Bharadwaj CP, Prasad KV, Raju DVS (2009). Studies on character association and path analysis of quantitative traits among parental lines of marigold (Tagetes erecta and T. patula) and their interspecific F1 hybrids. Indian Journal of Horticulture 66(3):348-352.

Qi YC, Zhou GL, Gao Y (2008). Study on squash technique of root tip and analysis of chromosome karyotype in Tagetes patula. Journal of Hubei University for Nationalities (Natural Science Edition) 26(3):261-265.

Sreekala C, Raghava SPS (2003). Exploitation of heterosis for carotenoid content in African marigold (Tagetes erecta L.) and its correlation with esterase polymorphism. Theoretical Applied Genetics 106(4):771-776.

Towner JW (1961). Cytogenetic studies on the origin of Tagetes patula. I. meiosis and morphology of diploid and allotetraploid T. erecta × T. tenuifolia. American Journal of Botany 48(9):743-751.

Wu RH, Ge BB, Wang ML, Zhou Y, Feng H (2016). Estimation of genome size of eighteen Chinese old garden roses by flow cytometry. Journal of Beijing Forestry University 38(6):94-100.

Zeng L, Zhao LJ, Sun J, Zhao ZG, Yang F (2010). Analysis of genetic relatedness of genetic resources of Tagetes as revealed by ISSR. Scientia Agricultura Sinica 43(1):215-222.

Zhang HL, Dong AX, Wang T, Zhao LJ, Xin HB (2014). A New Cultivar of Marigold ‘Jingyue 1’. Acta Horticulturae Sinica 41(7):1521-1522.

Zhu C (1982). Plant chromosome and chromosome technology. Beijing. Science Press.

Zhang et al., 2019. Identify Suitable Parents of Tagetes erecta and T. patula for Heterotic Hybrid Breeding
Published
2019-04-17
How to Cite
ZHANG, H., XIN, H., CONG, R., LI, Z., SONG, L., & WEI, Z. (2019). Cross Compatibility Analysis to Identify Suitable Parents of Tagetes erecta and T. patula for Heterotic Hybrid Breeding. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 676-682. https://doi.org/10.15835/nbha47311388
Section
Research Articles