Astaxanthin Production by Newly Isolated Rhodosporidium toruloides: Optimization of Medium Compositions by Response Surface Methodology


  • Tuyet Nhung TRAN Ton Duc Thang University, Faculty of Applied Sciences, Ho Chi Minh City 700000, Vietnam (VN)
  • Quang-Vinh TRAN 1) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam, Vietnam2) Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam (VN)
  • Hao Thanh HUYNH Ton Duc Thang University, Faculty of Applied Sciences, Ho Chi Minh City 700000, Vietnam (VN)
  • Nghia-Son HOANG 1) Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam, Vietnam2) Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City 700000, Vietnam (VN)
  • Hoang Chinh NGUYEN Ton Duc Thang University, Faculty of Applied Sciences, Ho Chi Minh City 700000, Vietnam (VN)
  • Dai-Nghiep NGO Vietnam National University-HCM, University of Science, Faculty of Biology and Biotechnology, Department of Biochemistry, Ho Chi Minh City 700000, Vietnam (VN)



astaxanthin production; Rhodosporidium toruloides; yeast; medium composition; response surface methodology


Astaxanthin is a valuable carotenoid pigment, which has been extensively used in various industries. In this study, Rhodosporidium toruloides was first used as a new microbial source for producing natural astaxanthin. Various carbon, nitrogen, and mineral sources were evaluated for their effect on astaxanthin production of R. toruloides. Response surface methodology (RSM) was then used to optimize the medium compositions for maximizing the astaxanthin concentration. Among the examined nutrients, glucose, peptone, and KH2PO4 were the most efficient carbon, nitrogen, and mineral source for astaxanthin production, respectively. Through RSM, a maximum astaxanthin concentration of 927.11 µg l-1 was obtained by using Hansen broth containing 83.74 g l-1 glucose, 20.01 g l-1 peptone, and 6.19 g l-1 KH2PO4. This study suggested that R. toruloides is a promising candidate to produce natural astaxanthin.


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How to Cite

TRAN, T. N., TRAN, Q.-V., HUYNH, H. T., HOANG, N.-S., NGUYEN, H. C., & NGO, D.-N. (2018). Astaxanthin Production by Newly Isolated Rhodosporidium toruloides: Optimization of Medium Compositions by Response Surface Methodology. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(2), 320–327.



Research Articles
DOI: 10.15835/nbha47111361

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