Low Level of Genetic Variation and Signs of Isolation in the Native Hungarian Sea Buckthorn Population Compared to Cultivated Specimens

  • Mária HÖHN Szent István University, Faculty of Horticultural Science, Department of Botany, H-1118 Budapest, 44 Ménesi street
  • Magdolna SZELÉNYI Plant Protection Institute, Department of Zoology, Centre for Agricultural Research, HAS Herman Ottó street 15, 1022 Budapest
  • Júlia HALÁSZ Szent István University, Faculty of Horticultural Science, Department of Genetics and Plant Breeding, H-1118 Budapest, 44 Ménesi street
Keywords: chalcone synthase intron; gender rate; gene reserve; Hippophae rhamnoides; phylogenetic tree; SCAR marker; SSR diversity

Abstract

Sea Buckthorn population from Újpest Nature Reserve, the only one assumed to be native in Hungary sustains at the periphery of Budapest city on the sandy substrate deposited by the Danube river. The study aims to characterize the gene stock preserved on this small edaphic habitat by comparing with the genetic material of planted specimens of known origin used in horticultural and gardening practice. Fragment length variations of seven nuclear microsatellite markers were evaluated and a phylogenetic tree was constructed based on sequence variation of an intron from nuclear gene encoding the chalcone synthase enzyme. Gender rate was evaluated by female specific SCAR marker. Microsatellite analysis pointed out the extremely low variation of the natural sea buckthorn population from Újpest Nature Reserve compared to the cultivated specimens, while the phylogenetic tree revealed that the natural population belongs to the genetic lineage distributed mostly in Western and Middle-Europe, and in Romania, region, which has strong links to the Balkan and the Black Sea. The analysis clearly separated the genetic material of the native Újpest population from the planted individuals from the vicinity that proved to be of allochthonous origin. The HrX2 SCAR marker revealed the dominance of the males (1:1.42). The study provides support for the autochthonous origin of the Hungarian remnant population being in reproductive isolation that suggest for its long persistence and relict status. Ex situ conservation and nature protection measures are highly recommended to maintain one of the last native sea buckthorn populations of the Pannonian region.

 

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In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue.

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Published
2019-05-15
How to Cite
HÖHN, M., SZELÉNYI, M., & HALÁSZ, J. (2019). Low Level of Genetic Variation and Signs of Isolation in the Native Hungarian Sea Buckthorn Population Compared to Cultivated Specimens. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3). https://doi.org/10.15835/nbha47311333
Section
Research Articles