The Proteome Response of Salt-Sensitive Rapeseed (Brassica napus L.) Genotype to Salt Stress


  • Nima DOLATABADI University of Tabriz, Faculty of Agriculture, Department of Plant Breeding and Biotechnology, Imam Khomeini Street, Tabriz, Iran
  • Mahmoud TOORCHI University of Tabriz, Faculty of Agriculture, Department of Plant Breeding and Biotechnology, Imam Khomeini Street, Tabriz, Iran
  • Mostafa VALIZADEH University of Tabriz, Faculty of Agriculture, Department of Plant Breeding and Biotechnology, Imam Khomeini Street, Tabriz, Iran
  • Ali BANDEHAGH University of Tabriz, Faculty of Agriculture, Department of Plant Breeding and Biotechnology, Imam Khomeini Street, Tabriz, Iran



abiotic stress, proteomics, rapeseed, salinity, two-dimensional electrophoresis


Productivity of rapeseed (Brassica napus L.), the third most important oilseed crop, was reduced more than other crops under the salt stress higher than the threshold. Thus, breeding, especially at seedling stage, seems necessary. Plants under salt stress, by synthesis of essential metabolites, specific structural proteins or enzymes of metabolic pathways deal with the stress. To identify the molecular mechanisms of salt responsiveness in rapeseed, ‘Option500’ a salt-sensitive genotype was exposed to 0, 150, and 300mM NaCl during the seedling stage. An increase in proline and the Na+ content of leaf and a reduction in shoot dry weight, plant height, K+ content and K+/Na+ ratio were observed. Protein expression changes were examined by two-dimensional electrophoresis (2-DE). Out of 110 protein spots identified by 2-DE gels, 37 spots showed significant abundant changes based on induction factor (IF), and 7 spots were recognized significantly at 5% probability level, which 1 and 6 spots were up and down-regulated, respectively. By using LC-MS/MS mass spectrometry analysis, proteins were identified which are involved in energy production and photosynthesis. Activity of enzymes involved in energy production decreased under stress, while the abundance of Phosphoribulokinase (PRK) -an important enzyme in the pentose phosphate pathway- increased.


Metrics Loading ...


Ashraf M, Akram NA (2009). Improving salinity tolerance of plants through conventional breeding and genetic engineering: An analytical comparison. Biotechnology Advances 27(6):744-752.

Ashraf M, McNeilly T (2004). Salinity Tolerance in Brassica Oilseeds. Critical Reviews in Plant Sciences 23(2):157-174.

Bahrani A (2013). Effect of salinity on growth , ions distribution, accumulation and chlorophyll concentrations in two canola (Brassica napus L.) cultivars. American-Eurasian Journal of Agricultural & Environmental Sciences 13(5):683-689.

Banaei-Asl F, Bandehagh A, Uliaei ED, Farajzadeh D, Sakata K, Mustafa G, Komatsu S (2015). Proteomic analysis of canola root inoculated with bacteria under salt stress. Journal of Proteomics 124:88-111.

Bandeh-hagh A, Toorchi M, Mohammadi A, Chaparzadeh N, Salekdeh GH, Kazemnia H (2008). Growth and osmotic adjustment of canola genotypes in response to salinity. Journal of Food, Agriculture and Environment 6(2):201-208.

Bandehagh A, Salekdeh GH, Toorchi M, Mohammadi A, Komatsu S (2011). Comparative proteomic analysis of canola leaves under salinity stress. Proteomics 11(10):1965-1975.

Bates LS (1973). Rapid determination of free proline for water - stress studies. Plant and Soil 39:205-207.

Benincasa P, Pace R, Quinet M, Lutts S (2013). Effect of salinity and priming on seedling growth in rapeseed (Brassica napus var oleifera Del.). Acta Scientiarum. Agronomy 35(4):479-486.

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2):248-254.

Caruso G, Cavaliere C, Guarino C, Gubbiotti R, Foglia P, Laganà A (2008). Identification of changes in Triticum durum L. leaf proteome in response to salt stress by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Analytical and Bioanalytical Chemistry 391(1):381-390.

Dolatabadi N, Toorchi M, Shakiba M-R, Kazemnia H, Komatsu S (2012). The response and protein pattern of spring rapeseed genotypes to sodium chloride stress. African Journal of Agricultural Research 7(5):755-763.

Dolatabadi N, Toorchi M, Valizadeh M, Bandehagh A (2016). Effect of salinity stress on some physiological traits of spring rapeseed genotypes at seedling stage. Journal of Biodiversity and Environmental Sciences 9(6):135-142.

Farhoudi R, Sharifzadeh F (2006). The effects of NaCl priming on salt tolerance in canola (Brassica napus L.) seedlings grown under saline conditions. Indian Journal of crop science 1:74-78.

Gao L, Yan X, Li X, Guo G, Hu Y, Ma W, Yan Y (2011). Proteome analysis of wheat leaf under salt stress by two-dimensional difference gel electrophoresis (2D-DIGE). Phytochemistry 72(10):1180-1191.

Giannakoula A, Ilias IF (2013). The effect of water stress and salinity on growth and physiology of tomato (Lycopersicon esculentum Mil.). Archives of Biological Sciences 65(2):611-620.

Gul H, Ahmed R, Hamayun M, Qasim M (2014). Growth Performance of Canola Grown Under Different Salinity Regimes. International Journal of Emerging Technology and Advanced Engineering 4(8):59-68.

Gunstone FD (2004). Rapeseed and canola oil: production, processing, properties and uses. Blackwell Publishing Ltd.

Guo G, Ge P, Ma C, Li X, Lv D, Wang S, Ma W, Yan Y (2012). Comparative proteomic analysis of salt response proteins in seedling roots of two wheat varieties. Journal of Proteomics 75(6):1867-1885.

Hajiaghaei Kamrani M, Hosseinniya H, Azam R chegeni (2013). Effect of salinity on the growth characteristics of canola (Brassica napus L.). Technical Journal of Engineering and Applied Sciences 3(18):2327-2333.

Haq TU, Akhtar J, Ali A, Maqbool MM, Ibrahim M (2014). Evaluating the response of some canola (Brassica napus L.) cultivars to salinity stress at seedling stage. Pakistan Journal of Agricultural Sciences 51(3):571-579.

Huseynova IM, Suleymanov SY, Aliyev JA (2007). Structural–functional state of thylakoid membranes of wheat genotypes under water stress. Biochimica et Biophysica Acta (BBA) - Bioenergetics 1767(6):869-875.

Jamil M, Rehman S ur, Rha ES (2014). Response of growth, PSII photochemistry and chlorophyll content to salt stress in four Brassica species. Life Science Journal 11(3):139-145.

Jiang Y, Yang B, Harris NS, Deyholos MK (2007). Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots. Journal of Experimental Botany 58(13):3591-3607.

Joseph B, Jini D (2010). Proteomic analysis of salinity stress-responsive proteins in plants. Asian Journal of Plant Sciences 9(6):307-313.

Joseph B, Jini D, Sujatha S (2010). Biological and physiological perspectives of specificity in abiotic salt stress response from various rice plants. Asian Journal of Agricultural Sciences 2(3):99-105.

Kamal AHM, Kim KH, Shin KH, Choi JS, Baik BK, Tsujimoto H, Heo HY, Park CS, Woo SH (2010). Abiotic stress responsive proteins of wheat grain determined using proteomics technique. Australian Journal of Crop Science 4(3):196-208.

Kandil AA, Sharief AE, Abido WAE, Ibrahim MMO (2012). Response of some canola cultivars (Brassica napus L.) to salinity stress and its effect on germination and seedling properties. Journal of Crop Science 3(3):95-103.

Kang G, Li G, Zheng B, Han Q, Wang C, Zhu Y, Guo T (2012). Proteomic analysis on salicylic acid-induced salt tolerance in common wheat seedlings (Triticum aestivum L.). Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1824(12):1324-1333.

Mer RK, Prajith PK, Pandya DH, Pandey AN (2000). Effect of salts on germination of seeds and growth of young plants of Hordeum vulgare, Triticum aestivum, Cicer arietinum and Brassica juncea. Journal of Agronomy and Crop Science 185(4):209-217.

Miyamoto S, Oster MF, Rostle CT, Lenn EG (2012). Salt tolerance of oilseed crops during establishment. Journal of Arid Land Studies 22(1):147-151.

Munns R. 2002. Comparative physiology of salt and water stress. Plant, Cell and Environment 25:239-250.

Murad AM, Molinari HBC, Magalhães BS, Franco AC, Takahashi FSC, De Oliveira NG, Franco OL, Quirino BF (2014). Physiological and proteomic analyses of Saccharum spp. grown under salt stress. PLoS ONE 9(6):1-12.

Nayyar H (2003). Accumulation of osmolytes and osmotic adjustment in water-stressed wheat (Triticum aestivum) and maize (Zea mays) as affected by calcium and its antagonists. Environmental and Experimental Botany 50(3):253-264.

Parida AK, Das AB (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety 60(3):324-349.

Parihar P, Singh S, Singh R, Singh VP, Prasad SM. 2014. Effect of salinity stress on plants and its tolerance strategies: a review. Environmental Science and Pollution Research 22(6):4056-4075.

Podda A, Checcucci G, Mouhaya W, Centeno D, Rofidal V, Del Carratore R, Luro F, Morillon R, Ollitrault P, Maserti BE (2013). Salt-stress induced changes in the leaf proteome of diploid and tetraploid mandarins with contrasting Na+ and Cl- accumulation behaviour. Journal of Plant Physiology 170(12):1101-1112.

Purty RS, Kumar G, Singla-Pareek SL, Pareek A (2008). Towards salinity tolerance in Brassica: An overview. Physiology and Molecular Biology of Plants 14(1-2):39-49.

Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002). A proteomic approach to analyzing drought-and salt-responsiveness in rice. Field Crops Research 76(2):199-219.

Sharma S, Mustafiz A, Singla-Pareek S, Shankar Srivastava P, Sopory S (2012). Characterization of stress and methylglyoxal inducible triose phosphate isomerase (OscTPI) from rice. Plant Signaling & Behavior 7(10):1337-1345.

Shirazi MU, Rajput MT, ANSARI R, Khan MA, TAHIR SS (2011). Salt tolerance in Brassica species at early seedling stage. Sindh University Research Journal-SURJ (Science Series) 43(2):203-208.

Sobhanian H, Razavizadeh R, Nanjo Y, Ehsanpour AA, Jazii FR, Motamed N, Komatsu S (2010). Proteome analysis of soybean leaves, hypocotyls and roots under salt stress. Proteome science 8(19):1-15.

Sobhanian H, Aghaei K, Komatsu S (2011). Changes in the plant proteome resulting from salt stress: Toward the creation of salt-tolerant crops? Journal of Proteomics 74(8):1323-1337.

Tanou G, Job C, Rajjou L, Arc E, Belghazi M, Diamantidis G, Molassiotis A, Job D (2009). Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity. Plant Journal 60(5):795-804.

Toorchi M, Dolati M, Adalatzadeh-Aghdam S (2014). Differentially expressed proteins in canola leaf induced by salt stress-a proteomic approach. International Journal of Biosciences 5(9):433-442.

Turan S, Cornish K, Kumar S (2012). Salinity tolerance in plants: breeding and genetic engineering. Australian Journal of Crop Science 6(9):1337-1348.




How to Cite

DOLATABADI, N., TOORCHI, M., VALIZADEH, M., & BANDEHAGH, A. (2018). The Proteome Response of Salt-Sensitive Rapeseed (Brassica napus L.) Genotype to Salt Stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(1), 17–23.



Research Articles
DOI: 10.15835/nbha47111133