Antioxidant Activity, Sugar Content and Phenolic Profiling of Blueberries Cultivars: A Comprehensive Comparison
Commercial blueberry production has been a viable industry throughout the world for 95 years; because of blueberry is a good source of antioxidant. Blueberries are especially rich in anthocyanin, a flavonoid with potent antioxidant capacity. The aim of this study was to compare the phenolic quantities, antioxidant activities, anthocyanin, sugar and phenolic compounds of blueberries produced in Turkey with those of similar blueberry varieties produced around the world. As a result of the conducted analysis, the total phenolic content (TPC) amount found in the berries was 77.26-215.12 mg GAE/100 g, the total flavonoid content (TFC) was 30.44-91.69 mg QE/100 g and the total anthocyanin content (TAC) was 43.03-295.06 mg c3-GE/100 g. Examining the antioxidant activities of the berries, DPPH between 1.10-5.65 mg/ml, FRAP between 454.93-36832.96 µmol troloks/100 g, β-Carotene between 40.66-86.48%. It was determined that the natural berries contained much more phenolic compounds and higher antioxidant activity than that of the cultivars The result of HPLC analysis, chlorogenic acid is determined to be the dominant compound in all berries. Furthermore, fructose and glucose are found in all fruits in different quantities while sucrose is found in certain varieties of berries as well. At the end of the performed study the data indicate that wild and cultivars of blueberries are rich sources of antioxidants for local as well international industries importing this fruit for food processing and enormous products.
Akerström A, Jaakola L, Bang U, Jaderlund A (2010). Effect of latitude-related factors and geographical origin on anthocyanidin concentration in fruits of Vaccinium myrtillus L. (bilberries). Journal of Agriculture and Food Chemistry 58(23):11939-11945.
Ayaz FA, Kadioglu A, Bertoft C, Acar C, Turna I (2001). Effect of fruit maturation on sugar and organic acid composition in two blueberries (V. arctostaphylos and V. myrtillus) native to Turkey. New Zealand Journal of Crop and Horticultural Science 29:137-141.
Benzie IFF, Strain JJ (1999). Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modi?ed version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299:15-27.
Brambilla A, Scalzo RL, Bertolo G, Torreggiani D (2008). Steam-blanched highbush blueberry (Vaccinium corymbosum L.) juice: phenolic profile and antioxidant capacity in relation to cultivar selection. Journal of Agricultural and Food Chemistry 56(8):2643-2648.
Bunea A, Rugina DO, Pintea AM, Sconta Z, Bunea CI, Socaciu C (2011). Comparative polyphenolic content and antioxidant activities of some wild and cultivated blueberries from Romania. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 39(2):70-76.
Can Z, Yildiz O, Sahin H, Turumtay EA, Silici S, Kolayli S (2015). An investigation of Turkish honeys: Their physico-chemical properties, antioxidant capacities and phenolic profiles. Food Chemistry 180:133-141.
Celik H (2009). The performance of some northen highbush blueberry (Vaccinium corymbosum L.) varieties in north eastern part of Anatolia. Anadolu Journal of Agriculture Science 24(3):141-146.
Connor AM, Luby JJ, Tong CBS, Finn CE, Hancock JF (2002). Genotypic and environmental variation in antioxidant activity, total phenolic content and anthociyanin content among blueberry cultivars. Journal of the American Society for Horticultural Science 127(1):89-97.
Ehlenfeldt MK, Prior RL (2001). Oxygen radical absorbance capacity (ORAC) and phenolic and anthocyanin concentrations in fruit and leaf tissues of highbush blueberry. Journal of Agricultural and Food Chemistry 49:2222-2227.
Ehret DL, Frey B, Forge T, Helmer T (2012). Effect of drip irrigation configuration and rate on yield and fruit quality of young highbush blueberry plants. HortScience 47(3):414-421.
Ercisli S, Celik H (2009). Mulberry and blueberry cultivation in Turkey. Pomologia Croatica 14(4):281-288.
Gao G, Draper E (2010). Growing blueberries in the home garden. Agriculture and Natural Resources 1-8.
Giovanelli G, Buratti S (2009). Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties. Food Chemistry 112:903-908.
Giusti MM, Wrolstad RE (2001). Anthocyanins characterization and measurement with UV-visible spectroscopy. In: Wrolstad R, Acree TE, An H, Decker EA, Penner MH, Reid DS, Schwartz S J, Shoemaker CF, Sporns P (Eds). Current Protocols in Food Analytical Chemistry, Unit F1.2. New York, John Wiley & Sons, Inc pp 1-13.
Häkkinen SH, Törrönen RA (2000). Content of flavonols and selected phenolic acids in strawberries and Vaccinium species: Influence of cultivar, cultivation site and technique. Food Research International 33:517-524.
Hatipoglu G, Sökmen M, Bektas E, Daferera D, Sökmen A, Demir E, Sahin H (2013). Automated and standard extraction of antioxidant phenolic compounds of Hyssopus officinalis L. ssp. angustifolius. Industrial Crops and Products 43:427- 433.
Hirvi T, Honkanen E (1983). The aroma of some hybrids between High-bush Blueberry (Vaccinium corymbosum, L.) and Bog Blueberry (Vaccinium uliginosum, L.). Zeitschrift fur Lebensmittel-Untersuchung und-Forschung 176:346-349.
Huang D, Ou B, Prior RL (2005). The chemistry behind antioxidant capacity assays. Journal of Agricultural and Food Chemistry 53:4303-4310.
Kafkas E, Kosar M, Türemis N, Baser KHC (2006). Analysis of sugars, organic acids and vitamin C contents of blackberry genotypes from Turkey. Food Chemistry 97:732-736.
Kafkas E, Özgen M, Özogul Y, Türemis N (2008). Phytochemical and fatty acid profile of selected red raspberry cultivars: A comparative study. Journal of Food Quality 31:67-78.
Kahkönen PM, Hopia AI, Heinonen M (2001). Berry phenolics and their antioxidant activity. Journal of Agricultural and Food Chemistry 49:4076-4082.
Kalt W, Dufour D (1997). Health functionality of blueberries. Journal of Horthtechnology 7(3):216-221.
Kalt W, Mcdonald JE, Donner H (2000). Anthocyanins, phenolics and antioxidant capacity of processed lowbush blueberry products. Journal of Agricultural and Food Chemistry 65:390-393.
Kalt W, Ryan DAJ, Duy JC, Prior RL, Ehlenfeldt MK, Kloet SPV (2001). Interspecific variation in anthocyanins, phenolics and antioxidant capacity among genotypes of highbush and lowbush blueberries (Vaccinium section cyanococcus spp.). Journal of Agricultural and Food Chemistry 49:4761-4767.
Kartal N, Sökmen M,Tepe B, Daferera D, Polissiou M, Sökmen A (2007). Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food Chemistry 100:584-589.
Koca I, Karadeniz B (2009). Antioxidant properties of blackberry and blueberry fruits grown in the Black Sea Region of Turkey. Scientia Horticulturae 121:447-450.
Lamaison JL, Carnat A (1991). Teneurs en principaux flavonoïdes des fleurs et des feuilles de Crataegus monogyna Jacq. et de Crataegus laevigata (Poiret) DC. En fonction de la période de végétation. Plantes Médicinales et Phytothérapie 25(1):12-16.
Lee J, Finn CE, Wrolstad RE (2004). Comparison of anthocyanin pigment and other phenolic compounds of Vaccinium membranaceum and Vaccinium ovatum native to the Pacific Northwest of North America. Journal of Agricultural and Food Chemistry. 52(23):7039-7044.
Marinova D, Ribarova F, Atanassova M (2005). Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of Chemical Technology and Metallurgy 40(3):255-260.
Nindo CI, Tang J, Powers JR, Singh P (2005). Viscosity of blueberry and raspberry juices for processing applications. Journal of Food Engineering 69:343-35.
Ochmian I, Kozos K, Chelpinski P, Szczepanek M (2015). Comparison of berry quality in highbush blueberry cultivars grown according to conventional and organic methods. Turkish Journal of Agriculture and Forestry 39:174-181.
Okan OT, Varlibas H, Öz M, Deniz I (2013). Antioxidant analysis methods and some non-wood forest plant products as sources of antioxidants in Eastern Black Sea region. Kastamonu University Journal of Forestry Faculty 13(1):48-59.
Peltola R (2013). Relevance of production area character?st?cs for NWFP demand and markets. In: Non-wood forest products, health and well-being. Publisher University of Helsinki Ruralia Institute: Espoo, Finland pp 35-36.
Pietta P, Gardana C, Pietta A (2003). Flavonoids in Herb. In: Evans CAR, Packer L (Eds). Flavonoids in Health and Disease. Marcel Dekker Inc, New York pp 43-69.
Prior LR, Cao G, Martin A, Sofic E, Mcewen J, O’Brien C, Lischner N, Ehlenfeldt M, Kalt W, Krewer G, Mainland MC (1998). Antioxidant capacity as influenced by total phenolic and anthocyanin content, maturity and variety of Vaccinium species. Journal of Agricultural and Food Chemistry 46:2686-2693.
Raghvendra VS, Sharma V, Shakya A, Hedaytullah MD, Arya GS, Mishra A, Gupta AG, Pachpute AP, Patel D (2011). Chemical and potential aspects of anthocyanins-a water-soluble vacuolar flavonoid pigments: A review. International Journal of Pharmaceutical Sciences Review and Research 6(1):28-33.
Ribera AE, Reyes-Diaz M, Alberdi M, Zuniga GE, Mora M L (2010). Antioxidant compound in skin and pulp of fruit changed among genotypes and maturity stages in highbush blueberry (Vaccinium corymbosum L.) grown in southern Chile. Journal of Soil Science and Plant Nutrition 10(4): 509-536.
Samad NB, Debnath T, Ye M, Hasna AM, Lim OB (2014). In vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts. Asian Pacific Journal of Tropical Biomedicine 4(10):807-815.
Sawa T, Nakao M, Akaike T, Ono K, Maeda H (1999). Alkylperoxyl radical-scavenging activity of various flavonoids and other phenolic compounds: Implications for the anti-tumor-promoter effect of vegetables. Journal of Agricultural and Food Chemistry 47:397-402.
Seeram NP, Adams LS, Zhang Y, Lee R, Sand D, Scheuller HS, Heber D (2006). Blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. Journal of Agricultural and Food Chemistry 54(25):9329-9339.
Singleton VL, Orthofer R, Raventos RML (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. In: Lester P (Ed). Methods in Enzymology. Academic Press 152-178.
Solovchenko A, Schmitz-Eiberger M (2003). Significance of skin flavonoids for UV?B?protection in apple fruits. Journal of Experimental Botany 54(389):1977-1984.
Stöhr H, Herrmann K (1975). The phenolics of fruits, VI. In: The phenolics of currants, gooseberries and blueberries. Changes in phenolic acids and catechins during development of black currants. Z Lebensm-Unters Forsch 159:31-37.
Taruscio TG, Barney DL, Exon J (2004). Content and profile of flavanoid and phenolic acid compounds in conjunction with the antioxidant capacity for a variety of northwest Vaccinium berries. Journal of Agricultural and Food Chemistry 52(10):3169-3176.
Uzelac VD, Savic Z, Brala A, Levaj B, Kavacevic DB, Bisko A (2010). Evaluation of phenolic content and antioxidant capacity of blueberry cultivars (Vaccinium corymbosum l.) grown in the Northwest Croatia. Food Technology and Biotechnology 48(2):214-221.
Yang G, Yue J, GongX, Qian B, Wang H, Deng Y, Zhao Y (2014). Blueberry leaf extracts incorporated chitosan coatings for preserving postharvest quality of fresh blueberries. Postharvest Biology and Technology 92:46-53.
Zheng W, Wang SY (2003). Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. Journal of Agricultural and Food Chemistry 51:502-509.
Zheng Y, Wang YC, Wang SY, Zheng W (2003). Effect of high-oxygen atmospheres on blueberry phenolics, anthocyanins, and antioxidant capacity. Journal of Agricultural and Food Chemistry 51:7162-7169.
Zimmer KR, Blum-Silva CH, Souza ALK, Wulffschuch M, Reginatto HF, Pereira CMP, Macedo AJ, Lencina LC (2014). The antibiofilm effect of blueberry fruit cultivars against Staphylococcus epidermidis and Pseudomonas aeruginosa. Journal of Medicinal Food 17(3):324-331.
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