Occurrence, Identification, and Pathogenicity of Fusarium spp. Associated with Tomato Wilt in Mexico


  • Micah R. ISAAC Universidad Autónoma Chapingo, Departamento de Fitotecnia, Km. 38.5 Carretera México-Texcoco, CP 56230 Chapingo, Estado de México (MX)
  • Santos G. LEYVA-MIR Universidad Autónoma Chapingo, Departamento de Parasitología Agrícola, Km. 38.5 Carretera México-Texcoco, CP 56230 Chapingo, Estado de México (MX)
  • Jaime SAHAGÚN-CASTELLANOS Universidad Autónoma Chapingo, Departamento de Fitotecnia, Km. 38.5 Carretera México-Texcoco, CP 56230 Chapingo, Estado de México (MX)
  • Kamila CÂMARA-CORREIA Universidade Federal do Cariri, Centro de Ciências Agrárias e da Biodiversidade 63133-610 Crato, Ceará (BR)
  • Juan M. TOVAR-PEDRAZA Universidad Autónoma Chapingo, Departamento de Parasitología Agrícola, Km. 38.5 Carretera México-Texcoco, CP 56230 Chapingo, Estado de México (MX)
  • Juan E. RODRÍGUEZ-PÉREZ Universidad Autónoma Chapingo, Departamento de Fitotecnia, Km. 38.5 Carretera México-Texcoco, CP 56230 Chapingo, Estado de México (MX)




characterization, Fusarium oxysporum complex, genotypes, ITS and EF-1?, phylogenetic tree, tomato


Fusarium wilt is considered as one of the most important diseases that affects tomato (Solanum lycopersicum L.) cultivation. The objective of this study was to identify and characterize Fusarium species with the potential to cause tomato wilt using morphological and molecular approaches, in order to generate the necessary information to achieve effective control of this disease. Fusarium isolates were found associated with commercial grown cultivars with disease incidence ranging from 10 to 85%. Forty isolates were identified by morphological characteristics as Fusarium oxysporum (38) and as Fusarium sp. (2). The isolates were evaluated for their pathogenicity on healthy tomato seedlings, which presented root rot at 20–35 days after inoculation. Fifteen of the most pathogenic isolates were analyzed with the internal transcribed spacer (ITS) region of DNA and the partial sequence of the translation elongation factor 1α (EF-1α). Isolates associated with tomato wilt, were identified molecularly as Fusarium oxysporum (13), Fusarium circinatum (1), and Fusarium andiyazi (1). Both analysis revealed that the mayor agent of tomato wilt in Mexico was F. oxysporum. This finding provides relevant information on tomato wilt in Mexico to decide the proper control methods for the pathogen.


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How to Cite

ISAAC, M. R., LEYVA-MIR, S. G., SAHAGÚN-CASTELLANOS, J., CÂMARA-CORREIA, K., TOVAR-PEDRAZA, J. M., & RODRÍGUEZ-PÉREZ, J. E. (2018). Occurrence, Identification, and Pathogenicity of Fusarium spp. Associated with Tomato Wilt in Mexico. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2), 484–493. https://doi.org/10.15835/nbha46211095



Research Articles
DOI: 10.15835/nbha46211095