Effect of Freeze, Oven and Microwave Pretreated Oven Drying on Color, Browning Index, Phenolic Compounds and Antioxidant Activity of Hawthorn (Crataegus orientalis) Fruit


  • Hacer COKLAR University of Selcuk, Faculty of Agricultural, Department of Food Engineering, Ardicli Street, 42035, Selcuklu, Konya (TR) http://orcid.org/0000-0002-4948-0960
  • Mehmet AKBULUT University of Selcuk, Faculty of Agricultural, Department of Food Engineering, Ardicli Street, 42035, Selcuklu, Konya (TR) http://orcid.org/0000-0001-5621-8293
  • Semih KILINC University of Selcuk, Faculty of Agricultural, Department of Food Engineering, Ardicli Street, 42035, Selcuklu, Konya (TR)
  • Ali YILDIRIM University of Selcuk, Faculty of Agricultural, Department of Food Engineering, Ardicli Street, 42035, Selcuklu, Konya (TR)
  • Iliasu ALHASSAN University of Selcuk, Faculty of Agricultural, Department of Food Engineering, Ardicli Street, 42035, Selcuklu, Konya (TR)




ABTS, browning index, color parameters (L*, a*, b*, C*, h), Crataegus orientalis, DPPH, phenolic compounds


Flowers, leaves and fruits of hawthorn plant are traditionally used for treating diseases like hypertension and atherosclerosis. The medicinal effects of the plant are generally attributed to its phenolic compounds. However, the fruits are perishable materials because of their high content of water, and generally dried and stored to be used outside its season. The main aim of this research was to investigate the effect of different drying methods on phenolic compounds of the hawthorn fruit. Fruits were collected from the wild growing trees in Turkey. De-seeded fruits were dried in freeze-, oven- (60 oC) and microwave pretreated oven drying (microwave application for 5 min at 360 W before drying at 60 oC) methods and analyzed for antioxidant activity, phenolic compounds, total phenolic content and color parameters. Total phenolic content of fresh hawthorn fruits was found as 13.36 mg g-1 DW. Oven- and microwave pretreated oven drying methods had a reductive effect on total phenolic content and antioxidant activity of fruits when compared to freeze drying method. (-)-Epicatechin (994.10 mg kg-1 DW), rutin (765.30 mg kg-1 DW), and procyanidin B2 (553.80 mg kg-1 DW) were the main phenolics of the fruit. Lowest values of these three compounds were observed in oven-dried fruits. Microwave pretreatment oven drying method resulted in browner product. Although the highest phenolic concentration and antioxidant activity were occurred in freeze-dried sample, microwave pretreatment before oven drying could be applied to reduce the time and cost of drying in terms of phenolic compounds and antioxidant activity.


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Annegowda H, Bhat R, Yeong KJ, Liong MT, Karim A, Mansor S (2014). Influence of drying treatments on polyphenolic contents and antioxidant properties of raw and ripe papaya (Carica papaya L.). International Journal of Food Properties 17(2):283-292.

Bondaruk J, Markowski M, Blaszczak W (2007). Effect of drying conditions on the quality of vacuum-microwave dried potato cubes. Journal of Food Engineering 81:306-312.

Brand-Williams W, Cuvelier ME, Berset C (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28(1):25-30.

Chang CH, Lin HY, Chang CY, Liu YC (2006a). Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. Journal of Food Engineering 77(3):478-485.

Chang Q, Zuo Z, Chow MS, Ho WK (2006b). Effect of storage temperature on phenolics stability in hawthorn (Crataegus pinnatifida var. major) fruits and a hawthorn drink. Food Chemistry 98(3):426-430.

Chong CH, Law CL, Figiel A, Wojdylo A, Oziemblowski M (2013). Colour, phenolic content and antioxidant capacity of some fruits dehydrated by a combination of different methods. Food Chemistry 141(4):3889-3896.

Ciurzynska A, Lenart A (2011). Freeze-drying-application in food processing and biotechnology-a review. Polish Journal of Food and Nutrition Sciences 61(3):165-171.

Coklar H, Akbulut M (2017). Anthocyanins and phenolic compounds of Mahonia aquifolium berries and their contributions to antioxidant activity. Journal of Functional Foods 35:166-174.

Çoklar H, Akbulut M (2016a). Alic (Crataegus orientalis) meyvesinin antioksidan aktivitesi ve fenolik bilesiklerinin ekstraksiyonu üzerine farkli cozgenlerin etkisi [Effect of different solvents on extraction of phenolic compounds and antioxidant activity of hawthorn (Crataegus orientalis) fruits]. Derim 33(2):221-232.

Çoklar H, Akbulut M (2016b). Olgunlasma ile alic (Crataegus orientalis) meyvesinin antioksidan aktivite, toplam fenolik madde ve fenolik profilindeki degisim [The change in antioxidant activity, total phenolic content and phenolic profile of hawthorn (Crataegus orientalis) fruit with maturity]. Fruit Science 3(2):30-37.

Edwards JE, Brown PN, Talent N, Dickinson TA, Shipley PR (2012). A review of the chemistry of the genus Crataegus. Phytochemistry 79:5-26.

Froehlicher T, Hennebelle T, Martin-Nizard F, Cleenewerck P, Hilbert JL, Trotin F, Grec S (2009). Phenolic profiles and antioxidative effects of hawthorn cell suspensions, fresh fruits, and medicinal dried parts. Food Chemistry 115(3):897-903.

Gao QH, Wu CS, Wang M, Xu BN, Du LJ (2012). Effect of drying of jujubes (Ziziphus jujuba mill.) on the contents of sugars, organic acids, a-tocopherol, b-carotene, and phenolic compounds. Journal of Agricultural and Food Chemistry 60(38):9642-9648.

Garcia-Mateos R, Ibarra-Estrada E, Nieto-Angel R (2013a). Antioxidant compounds in hawthorn fruits (Crataegus spp.) of Mexico. Revista Mexicana de Biodiversidad 84(4):1298-1304.

Garcia-Mateos R, Aguilar-Santelises L, Soto-Hernandez M, Nieto-Angel R (2013b). Flavonoids and antioxidant activity of flowers of Mexican Crataegus spp. Natural Product Research 27(9):834-836.

Gundogdu M, Ozrenk K, Ercisli S, Kan T, Kodad O, Hegedus A (2014). Organic acids, sugars, vitamin C content and some pomological characteristics of eleven hawthorn species (Crataegus spp.) from Turkey. Biological Research 47(1):21-26.

Jing Y, Chen JF, Zhao YY, Mao LC (2010). Effects of drying processes on the antioxidant properties in sweet potatoes. Agricultural Sciences in China 9(10):1522-1529.

Joshi A, Rupasinghe H, Khanizadeh S (2011). Impact of drying processes on bioactive phenolics, vitamin C and antioxidant capacity of red?fleshed apple slices. Journal of Food Processing and Preservation 35(4):453-457.

Krokida MK, Maroulis ZB, Saravacos GD (2001). The effect of the method of drying on the colour of dehydrated products. International Journal of Food Science and Technology 36(1):53-59.

Kumar D, Arya V, Bhat ZA, Khan NA, Prasad DN (2012). The genus Crataegus: Chemical and pharmacological perspectives. Revista Brasileira de Farmacognosia 22(5):1187-1200.

Kwok B, Hu C, Durance T, Kitts D (2004). Dehydration techniques affect phytochemical contents and free radical scavenging activities of Saskatoon berries (Amelanchier alnifolia nutt.). Journal of Food Science 69(3):122-126.

Liu P, Yang B, Kallio H (2010). Characterization of phenolic compounds in Chinese hawthorn (Crataegus pinnatifida Bge. var. major) fruit by high performance liquid chromatography-electrospray ionization mass spectrometry. Food Chemistry 121(4):1188-1197.

Maskan M (2001). Kinetics of colour change of kiwifruits during hot air and microwave drying. Journal of Food Engineering 48(2):169-175.

Mejia-Meza E, Yanez J, Remsberg C, Takemoto J, Davies N, Rasco B, Clary C (2010). Effect of dehydration on raspberries: Polyphenol and anthocyanin retention, antioxidant capacity, and antiadipogenic activity. Journal of Food Science 75(1):5-12.

Miletic N, Mitrovic O, Popovic B, Nedovic V, Zlatkovic B, Kandic M (2013). Polyphenolic content and antioxidant capacity in fruits of plum (Prunus domestica L.) cultivars ‘Valjevka’ and ‘Mildora’ as influenced by air drying. Journal of Food Quality 36(4):229-237.

Mohd-Zainol M, Abdul-Hamid A, Abu-Bakar F, Pak-Dek S (2009). Effect of different drying methods on the degradation of selected flavonoids in Centella asiatica. International Food Research Journal 16:531-537.

Nabavi SF, Habtemariam S, Ahmed T, Sureda A, Daglia M, Sobarzo-Sánchez E, Nabavi SM (2015). Polyphenolic composition of Crataegus monogyna Jacq.: From chemistry to medical applications. Nutrients 7(9):7708-7728.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved abts radical cation decolorization assay. Free Radical Biology and Medicine 26(9):1231-1237.

Rice-Evans C, Miller N, Paganga G (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science 2(4):152-159.

Sagar V, Kumar PS (2010). Recent advances in drying and dehydration of fruits and vegetables: A review. Journal of Food Science and Technology 47(1):15-26.

Shofian NM, Hamid AA, Osman A, Saari N, Anwar F, Pak Dek MS, Hairuddin MR (2011). Effect of freeze-drying on the antioxidant compounds and antioxidant activity of selected tropical fruits. International Journal of Molecular Sciences 12(7):4678-4692.

Singleton V, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3):144-158.

Sogi DS, Siddiq M, Dolan KD (2015). Total phenolics, carotenoids and antioxidant properties of Tommy Atkin mango cubes as affected by drying techniques. LWT-Food Science and Technology 62(1):564-568.

Vega-Mercado H, Gongora-Nieto MM, Barbosa-Canovas GV (2001). Advances in dehydration of foods. Journal of Food Engineering 49(4):271-289.

Wen L, Guo X, Liu RH, You L, Abbasi AM, Fu X (2015). Phenolic contents and cellular antioxidant activity of Chinese hawthorn Crataegus pinnatifida. Food Chemistry 186:54-62.

Wojdylo A, Figiel A, Lech K, Nowicka P, Oszmianski J (2014). Effect of convective and vacuum–microwave drying on the bioactive compounds, color, and antioxidant capacity of sour cherries. Food and Bioprocess Technology 7(3):829-841.

Wojdylo A, Figiel A, Oszmianski J (2009). Effect of drying methods with the application of vacuum microwaves on the bioactive compounds, color, and antioxidant activity of strawberry fruits. Journal of Agricultural and Food Chemistry 57(4):1337-1343.

Yanyang X, Min Z, Mujumdar AS, Le-qun Z, Jin-cai S (2004). Studies on hot air and microwave vacuum drying of wild cabbage. Drying Technology 22(9):2201-2209.

Zhang Z, Chang Q, Zhu M, Huang Y, Ho WK, Chen ZY (2001). Characterization of antioxidants present in hawthorn fruits. The Journal of Nutritional Biochemistry 12(3):144-152.




How to Cite

COKLAR, H., AKBULUT, M., KILINC, S., YILDIRIM, A., & ALHASSAN, I. (2018). Effect of Freeze, Oven and Microwave Pretreated Oven Drying on Color, Browning Index, Phenolic Compounds and Antioxidant Activity of Hawthorn (Crataegus orientalis) Fruit. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2), 449–456. https://doi.org/10.15835/nbha46211027



Research Articles
DOI: 10.15835/nbha46211027