Exogenous Carbon Magnifies Mycorrhizal Effects on Growth Behaviour and Sucrose Metabolism in Trifoliate Orange
Keywords:arbuscular mycorrhizal fungi, citrus, fructose, sucrose invertase, sucrose synthase
Arbuscular mycorrhizas (AMs) need the carbohydrates from host plants for its growth, whereas it is not clear whether exogenous carbon affects mycorrhizal roles. A two-chambered rootbox was divided into root + hyphae chamber and hyphae chamber (free of roots) by 37-μm nylon mesh, in which trifoliate orange (Poncirus trifoliata) seedlings and Funneliformis mosseae were applied into root + hyphae chamber, and exogenous 40 mmol/L fructose, glucose and sucrose was applied to hyphae chamber. Application of exogenous sugars dramatically elevated root mycorrhizal colonization. Sole arbuscular mycorrhizal fungi (AMF) inoculation significantly promoted plant growth and root morphology than non-AMF treatment. Mycorrhiza-improved plant growth and root modification could be enlarged by exogenous carbon, especially fructose. Exogenous carbon markedly increased root fructose, glucose and sucrose accumulation in mycorrhizal plants, especially sucrose. Exogenous fructose significantly reduced leaf and root sucrose synthase (SS) activity in synthesis direction and increased them in cleavage direction in AMF seedlings. Exogenous glucose and sucrose heavily elevated root SS activity of mycorrhizal seedlings in synthesis and cleavage direction and reduced leaf SS activity in synthesis direction. Leaf acid invertase (AI) and neutral invertase (NI) activities of mycorrhizal seedlings were decreased by exogenous carbon, except sucrose in NI. Exogenous fructose significantly increased root AI and NI activity in mycorrhizal plants. These results implied that mycorrhizal inoculation represented positive effects on plant growth, root morphology, and sucrose metabolism of trifoliate orange, which could be magnified further by exogenous carbon, especially fructose.
Bago B, Pfeffer PE, Shachar-Hill Y (2000). Carbon metabolism and transport in arbuscular mycorrhizas. Plant Physiology 124(3):949-958.
Bago B, Zipfel W, Williams RM, Jun J, Arreola R, Lammers PJ, … Shachar-Hill Y (2002). Translocation and utilization of fungal storage lipid in the arbuscular mycorrhizal symbiosis. Plant Physiology 128(1):108-124.
Bago B, Pfeffer PE, Abubaker J, Jun J, Allen JW, Brouillette J, … Shachar-Hill Y (2003). Carbon export from arbuscular mycorrhizal roots involves the translocation of carbohydrate as well as lipid. Plant Physiology 131(3):1496-1507.
Baier MC, Keck M, Godde V, Niehaus K, Kuster H, Hohnjec N (2010). Knockdown of the symbiotic sucrose synthase MtSucS1 affects arbuscule maturation and maintenance in mycorrhizal roots of Medicago truncatula. Plant Physiology 152(2):1000-1014.
Bécard G, Piché Y (1989). Fungal growth stimulation by CO2 and root exudates in vesicular-arbuscular mycorrhizal symbiosis. Applied and Environmental Microbiology 55:2320-2325.
Eveland AL, Jackson DP (2011). Sugars, signalling, and plant development. Journal of Experimental Botany 63(9):3367-3377.
Ferrol N, Pérez-Tienda J (2009). Coordinated nutrient exchange in arbuscular mycorrhiza. In: Azcón-Aguilar C, Barea JM, Gianinazzi S, Gianinazzi-Pearson V (Eds). Mycorrhizas-Functional Processes and Ecological Impact. Springer, Berlin Heidelberg pp 73-87.
Gadkar V, David-Schwartz R, Kunik T, Kapulnik Y (2001). Arbuscular mycorrhizal fungi colonization. Factors involved in host recognition. Plant Physiology 127(4):1493-1499.
He ZQ, He CX, Zhang ZB, Zou RZ, Wang HS (2006). Effects of various arbuscular mycorrhizal fungi on tomato growth and related physiological factors. Journal of Shenyang Agricultural University 37(3):308-312 (in Chinese with English abstract).
Hodge A, Campbell C, Fitter AH (2001). An arbuscular mycorrhizal fungus accelerates decomposition and acquisition nitrogen directly from organic material. Nature 413:297-299.
Jiang Y, Wang W, Xie Q, Liu N, Liu L, Wang D, … Wang E (2017). Plants transfer lipids to sustain colonization by mutualistic mycorrhizal and parasitic fungi. Science 356(6343):1172-1175.
Jin HR, Jiang DH, Zhang PH (2011). Effect of carbon and nitrogen availability on metabolism of amino acids in germinating spores of arbuscular mycorrhizal fungi. Pedosphere 21(4):432-442.
Lastdrager J, Hanson J, Smeekens S (2014). Sugar signals and the control of plant growth and development. Journal of Experimental Botany 65(3):799-807.
Lewis DH, Harley JL (1965). Carbohydrate physiology of mycorrhizal roots of beech. New Phytologist 64(2):224-237.
Luginbuehl LH, Menard GN, Kurup S, Van Erp H, Radhakrishnan GV, Breakspear A, … Eastmond PJ (2017). Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant. Science 356(6343):1175-1178.
Moscatello S, Famiani F, Proietti S, Farinelli D, Battistelli A (2011). Sucrose synthase dominates carbohydrate metabolism and relative growth rate in growing kiwifruit (Actinidia deliciosa, cv Hayward). Scientia Horticulturae 128(3):197-205.
O’Hara LE, Paul MJ, Wingler A (2013). How do sugars regulate plant growth and development? New insight into the role of trehalose-6-phosphate. Molecular Plant 6(2):261-274.
Phillips JM, Hayman DS (1970). Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society 55(1):158-161.
Schubert A, Allara P, Morte A (2004). Cleavage of sucrose in roots of soybean (Glycine max) colonized by an arbuscular mycorrhizal fungus. New Phytologist 161(2):495-501.
Verma AK, Agarwal AK, Dubey RS, Solomon S, Singh SB (2013). Sugar partitioning in sprouting lateral bud and shoot development of sugarcane. Plant Physiology and Biochemistry 62:111-115.
Wu QS, Peng YH, Zou YN, Liu CY (2010). Exogenous polyamines affect mycorrhizal development of Glomus mosseae colonized citrus (Citrus tangerine) seedlings. ScienceAsia 36:254-258.
Wu QS, Srivastava AK, Li Y (2015). Effect of mycorrhizal symbiosis on growth behavior and carbohdyrate metabolism of trifoliate orange under different substrate P levels. Journal of Plant Growth Regulation 34:495-508.
Wu QS, Zou YN, He XH, Luo P (2011). Arbuscular mycorrhizal fungi can alter some root characters and physiological status in trifoliate orange (Poncirus trifoliata L. Raf.) seedlings. Plant Growth Regulation 65:273-278.
Wu QS, Zou YN, Huang YM, Li Y, He XH (2013). Arbuscular mycorrhizal fungi induce sucrose cleavage for carbon supply of arbuscular mycorrhizas in citrus genotypes. Scientia Horticulturae 160:320-325.
Wu QS, Yuan FY, Fei YJ, Li L, Huang YM, Liu CY (2014). Effects of arbuscular mycorrhizal fungi on root system architecture and sugar contents of white clover. Acta Prataculturae Sinica 23(1):199-204.
Xie XL, Gu ZH, Zhu HH, Yao Q (2013). Correlation between glomalin related soil protein and root morphology. Mycosystema 32:993-1003 (in Chinese with English abstract).
Zobel RW, Alloush GA, Belesky DP (2006). Differential root morphology response to no versus high phosphorus, in three hydroponically grown forage chicory cultivars. Environmental and Experimental Botany 57(1):201-208.
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