Analysis of Genetic Diversity in 73 Kentucky Bluegrass Materials by SSR and SRAP Markers

Authors

  • Xiaojun YUAN Shanghai University, School of Life Science, Shanghai 200444 Shanghai University, Shanghai Key Laboratory of Bio-Energy Crops (CN)
  • Mingyue TU Shanghai University, School of Life Science, Shanghai 200444 Shanghai University, Shanghai Key Laboratory of Bio-Energy Crops (CN)
  • Yali HE Shanghai Jiaotong University, School of Agriculture and Biology, Shanghai 200240 (CN)
  • Weiqian WANG Saitama University, Graduate School of Science and Engineering, 255 Shimo-Okubo, Sakura-ku, Saitama City, Saitama 338-8570 (JP)
  • Jun LI Shanghai Academy of Agricultural Sciences, Shanghai 201403 (CN)
  • Shumin ZHOU Shanghai University, School of Life Science, Shanghai 200444 Shanghai University, Shanghai Key Laboratory of Bio-Energy Crops (CN)

DOI:

https://doi.org/10.15835/nbha46210916

Keywords:

cluster analysis, genetic resources, molecular markers, Poa pratensis L., turfgrass

Abstract

Kentucky bluegrass (Poa pratensisL.) (KBG) is a commonly used grass that possesses excellent quality, as well as a complex genetic background and reproductive patterns. In this study, a total of 73 KBG germplasms were collected, of which 49 were imported varieties, 5 were Chinese breeding varieties, and 19 were wild materials. A total of 70 simple sequence repeat (SSR) and 75 sequence-related amplification polymorphism (SRAP) markers were selected to use for genetic diversity analysis. From these studies, high levels of polymorphisms were observed in SRAPs (91.8%) and SSRs (94.5%), respectively. Three dendrograms that were generated from SRAP, SSR, and SRAP+SSR combined data revealed a general similarity for the positioning of the majority of materials. However, certain materials, including Z65, Z25, and Z27, were found to be located in diverse clusters among different dendrograms. Further analysis demonstrated no significant association between geographical origin and molecular marker clusters in the wild materials. Combined with the seedling phenotype identification carried out in our prior study, it seems as though there is no significant relationship between agronomic characterization and marker-based clustering in these materials, except for in the case of leaf color. These studies provided an increased understanding of genetic diversity among KBG materials, which will be beneficial for genetic improvement and germplasm conservation in the future.

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Published

2018-01-24

How to Cite

YUAN, X., TU, M., HE, Y., WANG, W., LI, J., & ZHOU, S. (2018). Analysis of Genetic Diversity in 73 Kentucky Bluegrass Materials by SSR and SRAP Markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 46(2), 327–335. https://doi.org/10.15835/nbha46210916

Issue

Vol. 46 No. 2 (2018)

Section

Research Articles
CITATION
DOI: 10.15835/nbha46210916

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Copyright (c) 2018 Xiaojun YUAN, Mingyue TU, Yali HE, Weiqian WANG, Jun LI, Shumin ZHOU

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