The Influence of Temperature, Light and Cytokinins on DNA Content of Musa acuminata (AAA Group) 'Kluai hom thong' and Musa balbisiana (BBB Group) 'Kluai hin'
Keywords:flow cytometry, Musa acuminata, organogenesis, content, Musa balbisiana, triploid banana
The changes in DNA content of triploid bananas Musa acuminata (AAA group) 'Kluai Hom Thong' and Musa balbisiana (BBB group) 'Kluai Hin' under different environmental conditions were investigated. The results revealed showed that different temperatures and light regimes had no effects on DNA content of both bananas cultivated on MS (Murashige and Skoog, 1962) medium supplemented with 5 mg/l BA (N6-benzyladenine) and 15% coconut water. However, they had some influences on morphological characteristics. On the other hand, both bananas cultivated on MS medium containing different concentrations of TDZ (N-phenyl-1, 2, 3 thidiazol-5-ylurea; thidiazuron) and BA, differed in their DNA contents. Increase in TDZ and BA concentrations resulted in the decrease of DNA content in 'Kluai Hom Thong', while the DNA content of 'Kluai Hin' increased.
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