Cultural, Morphological and Pathogenic Characterization of Alternaria porri Causing Purple Blotch of Onion

Abstract

Twenty seven (27) isolates of Alternaria porri were isolated from diseased leaf samples collected from different onion growing regions of Bangladesh and characterized for cultural, morphological and pathogenic variabilities. A. porri colonies colony colour ranged between light to dark olivacious and grayish white with irregular, regular with concentric ring and regular without concentric ring shape. Margin of colonies were entire, irregular and wavy with effuse, fluffy and velvety texture. Isolates impregnated media with colour ranged between grey to brown on the reverse of the plates. Growth rate of isolates ranged between 2.433 and 3.950 mm/day with fast growth in isolate DSTR 02 and least in MMBH. Morphological variation in conidia production was between 7.720×10³ to 47.02×10³ per mm² with sporulation time 3.33 to 11.00 days. The conidial shape was straight to curve with light to deep brown colour. The number of horizontal and vertical separation in the conidia ranged from 3.00 to 6.00 × 1.00 to 2.00 with size from 11.20 to 39.20 × 4.76 to 11.43 µm. In pathogenicity test isolates also exhibited variations in size of the lesions (2.77 to 7.55 mm) produced on onion leaves. The results demonstrate existence of considerable variation in cultural, morphological, and pathogenic characters of A. porri isolates prevalent in Bangladesh environment.