Exponential fertilization on red-seed tree (Ormosia hosiei) seedlings subjected to contrasting light conditions: Do we really need intensive nutrient loading?

Exponential fertilization (EF) can feed seedlings with more nutrients than needed for growth and continuous lighting is expected to reduce the risk of loading at high application rate. In this study, red-seed tree (Ormosia hosiei Hemsley & E. H. Wilson) seedlings were raised by EF at a low rate of 160 mg nitrogen (N) plant (conventional) (N-phosphorus [P]-potassium [K], 12-9-12) and a high rate of 600 mg N plant (intensive), which was adapted from previous studies with large-pot-seedlings. Both fertilizer regimes were nested to either of two light spectra from high-pressure sodium (HPS) and light-emitting diode (LED) sources with three replicates for each combined treatment. Seedlings subjected to the conventional regime in the LED spectrum showed better growth and greater biomass accumulation with higher leaf N and P contents than other ones. In the conventional fertilizer regime, the LED spectrum also resulted in higher photosynthesis shown by more pigments and higher N synthesis than the HPS spectrum. The HPS spectrum strengthened P synthesis in the intensive regime. The antioxidative activity was stimulated by a high dose of EF, hence excessive toxicity was likely induced. We recommend using the normal rate of 160 mg N plant for the culture of redseed tree seedlings with LED lighting to promote seedling quality without causing excessive nutrient toxicity.

2 stocks can quickly fulfill the requirement of big recruitment while maintaining genetic diversity. Fertilization during nursery culture has arisen due to its benefit in strengthening nutritional reserves in red-seed tree seedlings (Chu et al., 2020).
Nursery fertilization can promote the establishment of newly planted tree seedlings because an abundant nutrient storage would feed transplanted seedlings to overcome weed competition Jacobs et al., 2020). Exponential nutrient loading mostly synchronizes the pace of biomass accumulation in tree seedlings for most species Wang et al., 2016). Exponential fertilization (EF) has been well documented to promote seedling quality through enhancing nutrient uptake to a level higher than needed for growth demand (Hawkins et al., 2005;Xu et al., 2019). The goal of harvesting nutrient-enriched seedlings promotes the interest to feed seedlings with EF at an applicative rate as high as possible. Trials have been successful in delivering nutrients at high rates in larch (Larix olgensis)  and spruce (Picea mariana) (Boivin et al., 2004) seedlings. Information has been scarce about EF on vulnerable species at high rates. Because endangered species are vulnerable to environmental stress, they will likely be more sensitive to over-dose of fertilizer application than other species. The high sensitivity of red-seed tree seedlings to different fertilizer regimes makes its response unclear.
Currently, most seedlings that have been tested by EF came from timber species, such as larch , oak (Salifu and Jacobs, 2006;Uscola et al., 2015), spruce (Salifu and Timmer, 2003), and Buddhist pine (Podocarpus macrophyllus) (Xu et al., 2019). Luxury consumption of nutrients was usually shaped within an optimum range with the upper limit of a high rate of nitrogen (N) supply around 125 mg N plant -1 . The most intensive dose was found on Quercus ilex seedlings which ranged up to 200 mg N plant -1 (Uscola et al., 2015). These doses were used in studies with soluble fertilizers. Otherwise, controlled-release fertilizer (CRF) is a common and practical means to feed seedlings with the desired nutrient reserve for vulnerable species ( Li et al., 2017;Chu et al., 2019Chu et al., , 2020. The upper limit in studies using CRF for red-seed tree seedlings has been around 600 mg N plant -1 ( Li et al., 2017;Chu et al., 2020). Although the amount of fertilizer appeared to be much higher than fertigation delivery, the active uptake was low due to limited efficiency. The formula of nutrient delivery from CRF cannot fully synchronize the dynamic of nutrient demand according to growing dynamic. Nutrient loss from bottom leakage is hard to avoid and at least partly contributed to the low efficiency of nutrient delivery (Zhu et al., 2016). Therefore, an intensive regime of EF was still detectable under the condition that nutrients were delivered by fertigation.
Compared to the condition where all light sources are given by sunlight, a lighting environment with the unique source of artificial illumination was found to increase the nutritional demand in growing seedlings Zhu et al., 2016;Li et al., 2017). This is because continuous lighting promotes biomass accumulation, height growth will also be enhanced accordingly. These changes need more nutrient assimilation to fulfill the demand for synthesis and production. As a result, seedlings exposed to an extended photoperiod generally showed an enlarged appearance and uptake amount compared to those in a natural photoperiod. High-pressure sodium (HPS) lamps are one of the most common instruments used to achieve continuous lighting in greenhouse production of tree seedlings (Apostol et al., 2015;. Further development of light-emitting diode (LED) reveals that the modification of light spectra can further promote biomass accumulation and enlarge the demand of nutrient uptake (Luo et al., 2020;Zhao et al., 2020). Compared to the HPS spectrum, the use of LED spectrum on seedlings has shown additional benefits like promoted root development and active physiology (Li et al., 2018;Luo et al., 2020;. Therefore, the use of different light spectra in the extended photoperiod can facilitate successful feeding of red-seed tree seedlings with an intensive EF regime. In this study, red-seed tree seedlings were raised using an intensive dose of EF with the conventional dose as the control. One LED light spectrum was tested for its combined effect on seedling response in comparison to the HPS lamp light spectrum. We hypothesize that (i) intensive nutrient loading can be effective in promoting nutrient uptake by red-seed tree seedlings, and (ii) the LED light spectrum can generate a combined effect of growth and biomass promotion in addition to the intensive regime.

Plant material
Red-seed tree seeds were supplied by Research Institute of Subtropical Forestry, Chinese Academy of Forestry from a seed source in Anyue County (29°43' N, 105°25' E), Sichuan province, China. Seeds were transported to the Laboratory of Combined Manipulations of Illumination of Fertility on Plant Growth (Zhilunpudao Agric. S&T Ltd., Changchun, China) and soaked in distilled water for 17h. A potassium (K) permanganate solution (0.5%, w/w) was used to sterilize seeds for 0.5 h. Seeds were treated by bactericide (0.4%, w/w) and moved to a chamber for incubation at 30±4°C with relative humidity (RH) of 70%. About three months later all seeds swelled and germinated to show epicotyls. Germinant seeds were sown into growing media (55% peat, 20% spent-mushroom residue, 25% perlite; Mashiro-Dust TM , Zhiluntuowei A&F S&T Ltd., Changchun, China) in planting plugs (top diameter, 7cm; height, 13cm) embedded within plastic cultural trays. This growing container had the same size as those in studies on larch (Zhao et al., 2019), Buddhist pine Xu et al., 2019), and valued shrubs (Aralia elata) . The volume of our container was 0.5 L which was lower than that in the minimum pot but met the recommendation for the culture of red-seed tree seedlings (Chu et al., 2020). Basic properties of the substrate included 120 mg kg -1 NH4 + -N, 140 mg kg -1 NO3 --N and 360 mg kg -1 soluble phosphorus (P). More details about chemical properties of growing media can found in . The temperature was maintained within 15-36 °C while RH ranged from 28-99 %.

Fertilizer regime
One week after the emergence of the first pair of leaves, seedlings were subjected to EF. Two application rates were employed. A low rate of 160 mg N plant -1 was assigned to the conventional regime. This rate was close to the lowest amount (180 mg N plant -1 ) in CRF delivered to red-seed tree seedlings (Chu et al., 2020), but met the recommended optimum-rate (150-175 mg N plant -1 ) for the culture of Quercus ilex seedlings (Uscola et al., 2015). Another high rate of 600 mg N plant -1 was assigned to the intensive regime. This high rate was adapted from the normal one from the culture of another vulnerable species of Fragrant rosewood (Dalbergia odorifera) (Li et al., 2017;Li et al., 2018). This high rate also fell in the range of the highest rates (540-720 mg N plant -1 ) of CRF application for red-seed tree seedlings (Chu et al., 2020). Initial N content (NS) in seedlings was set to 1.4 mg plant -1 , which was necessary to calculate the coefficient (r) in EF model . Seedlings were fertigated once a week and a total of 16 applications were delivered. Thereafter, r was calculated to be 0.2967 for the delivery of 160 mg N plant -1 and 0.3789 for 600 mg N plant -1 according to the equation of Duan et al. (2013). Nutrients were delivered with chemical solutions of ammonium sulfate ([NH4]2SO4) and monopotassium phosphate (KH2PO4) (N-P-K, 12-9-12).

Light spectra
Seedlings started to receive lighting from the first day when over 80% of seedlings had shown their first pair of leaves. Half of the trayed seedlings were subjected to HPS lighting and the other half to LED lighting. The two light environments were split by a black-out fabric. In the HPS spectrum treatment, two lamps were fixed to a height of 60 cm above the ground and 45 cm to the tip of seedling shoots. LED light was given by a panel with diodes in red (600 to 700 nm), blue (400 to 500 nm), and green (500 to 600 nm) that combined supplied lights with the spectrum of 85% red, 10% green, and 5% blue light wavelengths 15 cm beneath the panel. Both illuminators emitted lights with photosynthetic photon flux density (PPFD) of 70-77 µmol m -2 s -1 and intensity of 2100-2700 Lx. More details about lighting condition and spectra characteristics can be found in .

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Seedling culture and sampling A total of 16 seedlings were raised in plugs of one tray with a 14 cm × 14 cm spacing. This equals to 8.82 × 10 3 individuals ha -1 . Seedlings were watered by a sub-irrigation system which was achieved by soaking from a tank . EF was applied through 16 deliveries; seedlings were sampled in the 17 th week after fertilizing began. Eight seedlings were randomly chosen and excavated from planting plugs in one tray. Height and root-collar diameter (RCD) were measured for the aerial parts of all eight sampled seedlings. Root stocks were kept intact to keep fine roots undamaged as much as possible. Roots were rinsed with distilled water to remove all adherent substrates. Cleaned seedlings were divided into two groups with one half used for measuring leaf area, biomass, nutrient concentration, and carbohydrate concentration, and the other half used for measuring chlorophyll, soluble protein, antioxidant enzyme activity, and nutrient assimilation enzyme activity.

Chemical analysis
Leaves were excised from the first half of four seedlings then seedlings were divided into stems and roots. Leaves were kept in moist towels until scanned and measured for projected area using digital analysis method (Xu et al., 2019). Samples of leaves, stem, and root were dried in an oven at 70±2 °C. Dried samples were measured for dry matter biomass weight, ground, and used to determine N and P concentrations  and soluble sugars and starch concentrations . Leaves of the other half of the four seedlings were excised and kept in soluble N until used to determine chlorophyl and soluble protein contents (Zhao et al., 2019) and GS and AP activities Zhao et al., 2020). Enzymatic antioxidant assays on superoxidase dismutase (SOD), peroxidase (POD), and catalase (CAT) were performed according to the methods in Hussain et al. (2018).

Statistical analysis
Data were analyzed using a split-plot design with different light spectra as the main block and fertilizer regime as the sub-block. Each light × fertilizer combined treatment was replicated three times and assigned as three trays of seedlings. The placement of trays was assigned as the random factor which was rearranged every week after fertigation. SAS software (Ver. 9.4, SAS Institute, Cary, NC, USA) was used to accomplish all statistical analyses. Analysis of variance (ANOVA) was employed using the split-plot model. When the combined effect was detected to be significant, data were analyzed by one-way ANOVA model in response to the combined light × fertilizer. Results were compared and arranged by Tukey test. The probability of significance was set as 0.05. Vector analysis was used to evaluate nutritional status according to the method of Salifu and Timmer (2003).

Seedling growth
Seedling height and RCD were both significant in response to the interactive effects of light spectra and fertilizer regime (Table 1). Seedlings subjected to the conventional fertilizer rate under LED lighting had the highest height ( Figure 1A). Height in seedlings exposed to a high rate of EF in HPS spectrum was the lowest. Root-collar diameter was also highest in seedlings subjected to a low rate of EF in LED spectrum ( Figure 1B). Seedlings subjected to an intensive regime in HPS spectrum had lower RCD than those in LED lighting.  H. Wilson) seedlings subjected to EF at low (conventional) and high (intensive) rates in spectra from highpressure sodium (HPS) lamps and in light-emitting diode (LED) panels Full lines in whisky-boxes present the medium value and the dashed lines present means. Different letters indicate significant differences (P<0.05) using Tukey test.

Biomass accumulation and nutrient content
All leaf, stem, and root biomasses were significant when subjected to combined light spectra and fertilizer regime treatments (Table 1). Biomass was greatest in leaves, stem, and root of seedlings subjected to EF at a low rate in the LED spectrum ( Figure 2A). However, the difference of stem biomass was not significant between LED-lighting for seedlings exposed to conventional vs intensive regimes. The LED spectrum resulted in a higher root to shoot biomass ratio (R/S) than the HPS spectrum (0.25±0.05 and 0.18±0.03, respectively).

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Nitrogen content was significant when subjected to combined treatments of fertilizer regime and light spectra (Table 1). Leaf N content was greatest in seedlings subjected to EF at a low rate in LED lighting ( Figure  2B). Stem N content showed nearly the same difference among treatments, but the difference was not significant between conventional and intensive regimes in the LED spectrum. Root N content was higher in seedlings subjected to EF at a low rate in the LED spectrum than in seedlings in combined intensive loading and HPS spectrum treatment.
Phosphorus content was significantly different across light spectrum × fertilizer treatments in leaves (Table 1). Again, seedlings subjected to EF at a low rate in the LED spectrum had higher P content than those subjected to a high rate in the HPS spectrum ( Figure 2C). Phosphorus content was not different across combined fertilizer × light treatments in stem and root (Table 1). However, the light spectrum had a significant main effect on P content in roots. Compared to seedlings subjected to the HPS spectrum (0.93±0.21mg plant -1 ; mean ± standard deviation, the same below), those in the LED spectrum had higher root P content (1.43±0.33 mg plant -1 ).

Nutrient concentration
Effects of light spectrum and fertilizer regime had a significant combined effect on N and P concentrations in most organs except for N concentration in leaves (Table 1). However, the light spectrum showed a main effect on leaf N concentration, which was higher in the LED spectrum (13.57±1.24 mg g -1 ) than in the HPS spectrum (11.68±1.47 mg g -1 ) by 16% ( Figure 3A). Stem N concentration was higher in seedlings subjected to the two fertilizer regimes in the LED spectrum than in those in the HPS spectrum ( Figure 3B). Root N concentration was higher in seedlings subjected to a high dose of EF in the HPS spectrum than in the low dose and LED combined treatment ( Figure 3C). Phosphorus concentration also showed the same response in the two treatments for all three types of organs ( Figure 3D-F). In addition, seedlings subjected to the two fertilizer regimes in the HPS spectrum had higher P content in stem than those in the LED spectrum ( Figure 3E). Root P concentration in the low rate and LED spectrum treatment was also lower than that in the low rate plus HPS spectrum treatment ( Figure 3F).

Diagnosis of nutritional status
Compared to EF at a low rate, that at a high rate resulted in a lower level of biomass in both types of light spectra (Figure 4). Leaf N content declined from the conventional regime to the intensive one and N concentration also decreased accordingly ( Figure 4A). Therefore, seedlings in the intensive regime were characterized with excessive N accumulation caused by antagonistic translocation. Although the HPS spectrum led to greater relative N decline from the conventional regime to the intensive regime, seedlings in the LED spectrum showed a significant and greater extent of leaf biomass decline. As a result, it can be concluded that the LED spectrum caused more severe relative N excess in the intensive regime than the HPS spectrum.
Relative to seedlings subjected to the conventional regime, those in the intensive regime suffered declines of leaf biomass accumulation and P content but a stimulation of leaf P concentration in the LED spectrum ( Figure 4B). Therefore, the relative P status in the LED spectrum can be characterized as excess P supply caused by toxic accumulation. Otherwise, the relative decline of biomass and P content and concentration in leaves in the HPS spectrum can be characterized with excess P supply caused by antagonistic translocation ( Figure 4B). With regard to the magnitude of vectors and the significant decline of biomass, the LED spectrum was again concluded to result in a more severe P excess when under an intensive fertilizer regime.  EF at a low rate (reference) and high rate (object) in contrasting spectra from high-pressure sodium (HPS) and light-emitting diode (LED) illuminators The establishments of vector shift and adherent interpretation are adapted from Salifu and Timmer (2003). Shift E, excess nutrient supply caused by toxic accumulation; Shift F, excess nutrient supply caused by antagonistic translocation.

Carbohydrate concentration
No interactive effect of light spectrum and fertilizer regime have been found on soluble sugar and starch concentrations in leaves (P=0.7517 and 0.1808, respectively) and roots (P=0.6671 and 0.9743, respectively). EF at a high rate resulted in higher starch concentration in root compared to that at a low rate ( Figure 5A). Accordingly, non-structural carbohydrate (NSC; soluble sugars plus starch) concentration was also higher in the intensive fertilizer regime than in the conventional regime. Soluble sugar concentration in leaves ranged from 126 to 129 mg g -1 and showed no response to any treatments. Soluble sugar concentration showed no 9 response to fertilizer regime. However, soluble sugar concentration was higher in the HPS spectrum than in the LED spectrum. NSC concentration was higher in the HPS spectrum than in the LED spectrum.

Physiological responses
Light spectrum and fertilizer regime had an interactive effect on chlorophyl a (P=0.0155), chlorophyl b (P=0.0419), and carotenoid (P=0.0472) concentrations. Seedlings in EF at a low rate in the LED spectrum had higher chlorophyl-a and carotenoid concentrations than seedlings subjected to the conventional regime in the HPS spectrum (Table 2). However, chlorophyl-b concentration was higher in seedlings subjected to the intensive regime than in the conventional regime in the HPS spectrum. Soluble protein concentration was also higher in the conventional regime in the LED spectrum than in the conventional regime in the HPS spectrum (P=0.0155). Leaf area was higher in seedlings subjected to EF at a low rate in the LED spectrum than in seedlings receiving two fertilizer regimes in the HPS spectrum (P=0.0059). Seedlings in the conventional regime in the LED spectrum had higher activity of GS than those in the HPS spectrum receiving two fertilizer regimes (P=0.0270). In contrast, the AP activity was higher in seedlings subjected to EF at a high rate in the HPS spectrum than in the conventional regime in the LED spectrum (P=0.0084). No interaction between light spectrum and fertilizer regime was detected for the effect on POD activity (P=0.1497) ( Figure 6A). Additionally, the light spectrum did not show any main effect on the POD activity (53-47 mmol min -1 mg -1 protein). However, EF at a high rate induced higher POD activity (58.70±11.32 mmol min -1 mg -1 protein) relative to a low rate (41.25±12.82 mmol min -1 mg -1 protein). Wilson) seedlings subjected to EF at low (conventional) and high (intensive) rates in spectra from highpressure sodium (HPS) lamps and in light-emitting diode (LED) panels Error bars present standard deviation. Different letters indicate significant differences (P<0.05) using Tukey test.

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The SOD activity was higher in seedlings subjected to EF at high rate in the LED spectrum than in those subjected to both types of fertilizer regimes in the HPS spectrum (P=0.0031) ( Figure 6B).
Among light spectra and fertilizer treatments, the CAT activity was highest in seedlings subjected to EF in the LED spectrum (P<0.0001) ( Figure 6C). Seedlings subjected to EF at a high rate in the HPS spectrum had higher CAT activity than those in the conventional + HPS treatment and in the conventional + LED treatment. Seedlings in EF at a low rate in the HPS spectrum had the lowest CAT activity ( Figure 6C).

Discussion
In our first hypothesis, we expected to promote nutrient uptake by EF at a high rate. This was put forward because the high application rate in the intensive regime of 600 mg N plant -1 fell within empirical cases of culturing tree seedlings in vulnerable species by CRF fertilization ( Li et al., 2017;Li et al., 2018;Chu et al., 2020). However, according to our findings we cannot accept our first hypothesis. The vector analysis indicated symptoms of excess supplies of both N and P elements in both spectra. This was characterized by the dual decline of leaf biomass and nutrient (N and P) content. Therefore, we can confirm that the high rate used in this study was not suitable for the culture of red-seed tree seedlings even though it has been used in CRF fertilization. As Zhu et al. (2016) found, leaching loss cannot be avoided when CRF was used as the source of nutrient supply. Seedlings did not show negative response when subjected to a high dose of CRF because some nutrients may have been lost by leaching. It was necessary to feed seedlings with a greater amount than needed of CRF granules to cover the loss. Although fertigation would result in nutrient loss through leaching (Dumroese et al., 2005), EF can maximize the efficiency of nutrient delivery which covers part of leaching loss.
We did not quantify nutrient leaching; hence, we cannot give any confirmative results about the amount of nutrient loss. Future work is suggested to test our conjecture with assessment of both nutrient uptake and leaching loss. The rate of 160 mg N plant -1 can be recommended to fertilize red-seed tree seedlings through EF. If more clarification is required on a more precise rate for a range of nutritional statuses, a dose-response model could be studied in future work wherein our recommended dose can be assumed as the optimum one (Uscola et al., 2015;Xu et al., 2019).
It was surprising to find that the over-dose of intensive loading can increase root NSC accumulation through promoting root starch translocation. The increase of root starch concentration facing a high stress environment has also been found for subalpine (Brunner et al., 2002) and citrus trees (Yang et al., 2020). Two possible explanations could account for our results. The first is that the toxicity supply broke enzyme activities that were responsible for starch hydrolysis (Devi et al., 2007). The second is that toxicity promoted the conversion from sucrose into starch to fuel stressed roots . Because soluble sugars in root were not affected by fertilizer regime, we agree more with the assumption that the access of starch hydrolysis was broken.
The excess of nutrient supply can also be reflected by shoot growth, which was reduced by the intensive fertilizer regime in the LED spectrum. The decrease of growth is another symptom indicating negative impact from over-dose fertilization (Xu et al., 2019). Stem growth and shoot biomass were not affected by the fertilizer treatment in the HPS spectrum. This means that the HPS spectrum cannot supply a lighting environment that facilitates the variation of shoot growth between fertilizer doses. It was interesting to find that nutrient uptake was also not affected by the EF treatment in the HPS spectrum. This suggests that the failure of shoot growth in response to fertilizer regime from lighting by the HPS was partly caused by low uptake. As the intensive loading was identified to cause nutrient excess, it can be surmised that the HPS spectrum "weakened" this negative effect by diminishing the effect of a high dose. Otherwise, the LED spectrum caused stimulation to it because most responses in growth, biomass, and nutrient uptake were differentiated. The LED spectrum was also found to promote tree seedling growth relative to the HPS spectrum , as well as promoting nutrient uptake and utilization compared to the HPS spectrum when the rhizosphere 12 nutrients are satisfied by roots (Luo et al., 2020;. Our results supplemented this judgement with new findings that the LED spectrum can also aggravate the symptom of excess nutrient supply. Therefore, we can accept our second hypothesis. In the LED spectrum, the low-rate-EF provided seedlings with higher ability of photosynthesis and N assimilation. Enhanced syntheses in pigment and protein are the result of promotion on N uptake and assimilation in tree seedlings in the LED spectrum (Apostol et al., 2015;. These changes suggest promoted photosynthesis in the LED spectrum which has also been reported on Buddhist pine (Luo et al., 2020) and coniferous seedlings (Apostol et al., 2015). Increased leaf area under LED lighting is the precondition to enlarge project area of the photobiological device and the subsequent promotion of photosynthetic rate. Increased leaf area in the LED spectrum compared to the HPS spectrum has also been found on bedding and vegetable plants (Poel and Runkle, 2017). Results about the AP activity concur with those about P concentration in seedling organs. Plant P concentration was found to have a positive relationship with soluble sugar concentration . Our P results were in accordance with soluble sugar concentration, both of which suggest that the HPS spectrum would be more beneficial for P uptake and soluble sugar synthesis.
The formation of reactive oxygen species (ROS) may be prevented by an antioxidant system with POD, SOD, and CAT working as ROS-scavenging compounds. We did not find significant difference of POD activity either among combined treatments or between light spectra. This disagreed with previous findings (He et al., 2015). Seedlings with a high rate of EF showed higher POD activity, suggesting a negative effect of nutrient excess. Both activities of SOD and CAT were higher in the LED spectrum and the intensive fertilizer regime than in seedlings subjected to the HPS spectrum. These results suggest that a high rate caused the ROS reaction and the LED spectrum aggravated the negative impact.

Conclusions
Red-seed tree seedlings show better growth and biomass performances in the LED spectrum than in the HPS spectrum, but the benefit was only apparent in combination with a low rate of EF. Although a high rate was adapted from studies with CRF fertilization to vulnerable seedlings, it was too high for red-seed tree seedlings which caused higher levels of antioxidants. Therefore, a low rate of 160 mg N plant -1 is recommended for the culture of red-seed tree seedlings with LED lighting to harvest excellent quality and corresponding physiological responses.

Authors' Contributions
Conceptualization: XC and ZZ; Data curation: XC; Formal analysis: XC; Funding acquisition: ZC; Investigation: XC and XL; Methodology: XC and ZZ; Project administration: ZC; Resources: ZC; Software: XC; Supervision: ZZ; Validation: XL; Visualization: XC; Writing -original draft: XC; Writing -review and editing: ZZ. Authorship must be limited to those who have contributed substantially to the work reported. All authors read and approved the final manuscript.