Determination of Camellia oleifera Abel. Germplasm Resources of Genetic Diversity in China using ISSR Markers

Junyong CHENG, Dezhi JIANG, Hua CHENG, Xihua ZHOU, Yingyou FANG, Xuehua ZHANG, Xian XIAO, Xianzhen DENG, Linling LI


Camellia oleifera is one of the four woody oil plants in the world, which is widely cultivated in South China. To examine the genetic diversity of C. oleifera in China, the diversity and genetic relationships among and within major populations of 109 varieties of C. oleifera were analyzed using ISSR markers. Twenty-three ISSR primers out of 49 primers yielded approximately 487 legible bands. A total of 335 of these bands were polymorphic markers, and the ratio of polymorphism was 68.86%. From the results, Zhejiang province showed the highest populations genetic diversity (H value 0.18), while Guangxi population showed the lowest genetic diversity (H 0.0851). Base on the bands, the genetic similarity coefficient ranged from 0.61 to 0.93 using NTSYS2.10e software. When coefficient was 0.75, 109 cultivars were divided into 11 categories and categories I contain 79 varieties by UPGMA cluster analysis. The test varieties divided into 7 sub-groups when categories were 0.75, which show a close genetic relationship. Results advised that Hunan is the main producing area of C. oleifera, with enriched C. oleifera variety and complex topography, and therefore has a high genetic diversity. Meanwhile, the main varieties of C. oleifera in Hubei are imported from Hunan, which results in fewer varieties and reduces the genetic diversity of C. oleifera. The ISSR profiles can improve C. oleifera germplasm management and provide potential determine correlations between different varieties and its distribution in different province.


germplasm management; genetic diversity; ISSR marker; polymorphism; population distribution

Full Text:



Cheng SY, Yuan HH, Cheng H, Li LL, Wang Y, Xu F, Jiang DZ (2012). A preliminary identification on the genetic resources of Luotian chestnut by ISSR markers. Hubei Agricultural Sciences 51:3096-3100.

Dai H, Zhao H, Jia G, Fan J, Sun Z, Sanqiao WU, Zhang X, Ren X, Zhao G, Wei A (2013a). Construction of molecular fingerprinting database for Camellia oleifera by ISSR markers. Acta Agriculturae Boreali-Occidentalis Sinica 22:101-107.

Dai HP, Zhao H, Jia GL, Fan JX, San-Qiao WU, Sun ZF, Wei AZ (2013b). Research advances on ISSR repeat fingerprints analysis to assess genetic diversity in Camellia oleifera. Journal of Northwest Forestry University 29:107-111.

Hamza H, Benabderrahim MA, Elbekkay M, Ferdaous G, Triki T, Ferchichi A (2012). Investigation of genetic variation in Tunisian date palm (Phoenix dactylifera L.) cultivars using ISSR marker systems and their relation with fruit characteristics. Turkish Journal of Biology 18:196-198.

Hu JL, Nie SP, Huang DF, Li C, Xie MY (2012). Extraction of saponin from Camellia oleifera cake and evaluation of its antioxidant activity. International Journal of Food Science & Technology 47:1676-1687.

Huang Y (2006). Analysis of genetic diversity in Camellia oleifera germplasms. Scientia Silvae Sinicae 42:38-43.

Jin X (2012). Bioactivities of water-soluble polysaccharides from fruit shell of Camellia oleifera Abel.: Antitumor and antioxidant activities. Carbohydrate Polymers 87:2198-2201.

Lee CP, Shih PH, Hsu CL, Yen GC (2007). Hepatoprotection of tea seed oil (Camellia oleifera Abel.) against CCl4-induced oxidative damage in rats. Food & Chemical Toxicology 45:888-895.

Liu Y, Liu DC, Wu B, Sun ZH (2006). Genetic diversity of pummelo (Citrus grandis Osbeck) and its relatives based on simple sequence repeat markers. Chinese Journal of Agricultural Biotechnology 3:119-126.

Liu Y, Peng Y, He R, Li L, Tang G, Wang C, Xu M, Zeng C, Tang H (2016). Genetic diversity of Camellia oleifera in hainan by ISSR. Molecular Plant Breeding 14:517-523.

Peng F, Yingying WU, Hao M, Chen L, Chen Y (2012). Genetic diversity of Camellia oleifera using ISSR and SRAP markers. Journal of Nanjing Forestry University 36:19-25.

Salis C, Papadakis IE, Kintzios S, Hagidimitriou M (2017). In vitro propagation and assessment of genetic relationships of citrus rootstocks using ISSR molecular markers. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 45:383.

Wang Y (2011). The researches on genetic diversity and varieties configuration of Camellia Oleifera. Master, Central South University of Forestry and Technology, Changsha, China.

Wen Q, Jin-Shan YE, Lei X, Jiang M, Huang LL, Jiang XM, Xu LC (2006). Study on the Inter-simple sequence repeat condition of Camellia oleifera. Journal of Central South Forestry University 26:22-26.

Wu Shuo, Fu JM, Wu Y, Liang YQ, Li FD (2012). Development and screening of EST-SSR markers in Diospyros kaki Thunb. Nonwood Forest Research 30:27-31.

Wu ZX, Wang HZ, Shi NN, Zhao Y (2008). The genetic diversity of Cymbidium by ISSR. Hereditas 30:627-632.

Yu XY, Yu FY, Liu J, Chen J (2013). Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker. Journal of Nanjing Forestry University 37:61-66.

Yang CF, Chen BL, Huang CM, LüWeiLi (2011). Isolation of genomic DNA and establishment of ISSR reaction system for Camellia crepnelliana Tutch. Journal of Southern Agriculture 42:233-235.

Yang CF, Chen BL, Huang CM, LüWeiLi (2013). ISSR analysis on genetic relation among 13 species of Camellia crepnelliana Tutch. Journal of Southern Agriculture 44:1421-1425.

Zhang Q, Luo Z (2004). ISSR technology and its applications in fruit trees. Journal of Fruit Science 2004-01.

Zhang T, Liu SQ, Dong YL (2011). Analysis of genetic basis of Camellia oleifera from Hubei province. Journal of Henan Agricultural Sciences 40:53-56.

Zhang WG, Zhang DC, Chen XY (2012). A novel process for extraction of tea oil from Camellia oleifera seed kernels by combination of microwave puffing and aqueous enzymatic oil extraction. European Journal of Lipid Science & Technology 114: 352-356.

Zhao Y, Ruan CJ, Ding GJ, Mopper S (2017). Genetic relationships in a germplasm collection of Camellia japonica and Camellia oleifera using SSR analysis. Genetics & Molecular Research 16:16019526.


June 1, 2018: Notulae Botanicae Horti Agrobotanici Cluj-Napoca in Scopus – Elsevier CiteScore 2017=0.78, Horticulture; Agronomy and Crop Science; Plant Science